TY - JOUR
T1 - Within-host evolutionary dynamics of seasonal and pandemic human influenza a viruses in young children
AU - Han, Alvin X.
AU - Garza, Zandra C. Felix
AU - Welkers, Matthijs R. A.
AU - Vigeveno, René M.
AU - Duong, Tran Nhu
AU - Mai, Le Thi Quynh
AU - Thai, Pham Quang
AU - Thoang, Dang Dinh
AU - Anh, Tran Thi Ngoc
AU - Tuan, Ha Manh
AU - Hung, Nguyen Thanh
AU - Thinh, Le Quoc
AU - Hai, Le Thanh
AU - Ngoc, Hoang Thi Bich
AU - Chokephaibulkit, Kulkanya
AU - Puthavathana, Pilaipan
AU - Chau, Nguyen Van Vinh
AU - Ngoc, Nghiem My
AU - van Kinh, Nguyen
AU - Trinh, Dao Tuyet
AU - Hien, Tran Tinh
AU - Wertheim, Heiman F. L.
AU - Horby, Peter
AU - Fox, Annette
AU - van Doorn, H. Rogier
AU - Eggink, Dirk
AU - de Jong, Menno D.
AU - Russell, Colin A.
N1 - Funding Information:
A.X.H., Z.C.F.G. and C.A.R. were supported by ERC NaviFlu (No. 818353). The South East Asia Infectious Disease Clinical Research Network (SEAICRN) was funded by National Institutes of Allergy and Infectious Diseases, National Institutes of Health (US), N01-A0-50042, HHSN272200500042C.
Publisher Copyright:
© 2021, eLife Sciences Publications Ltd. All rights reserved.
PY - 2021
Y1 - 2021
N2 - The evolution of influenza viruses is fundamentally shaped by within-host processes. However, the within-host evolutionary dynamics of influenza viruses remain incompletely understood, in part because most studies have focused on infections in healthy adults based on single timepoint data. Here, we analysed the within-host evolution of 82 longitudinally-sampled individuals, mostly young children, infected with A/H1N1pdm09 or A/H3N2 viruses between 2007 and 2009. For A/H1N1pdm09 infections during the 2009 pandemic, nonsynonymous minority variants were more prevalent than synonymous ones. For A/H3N2 viruses in young children, early infection was dominated by purifying selection. As these infections progressed, nonsynonymous variants typically increased in frequency even when within-host virus titres decreased. Unlike the short-lived infections of adults where de novo within-host variants are rare, longer infections in young children allow for the maintenance of virus diversity via mutation-selection balance creating potentially important opportunities for within-host virus evolution.
AB - The evolution of influenza viruses is fundamentally shaped by within-host processes. However, the within-host evolutionary dynamics of influenza viruses remain incompletely understood, in part because most studies have focused on infections in healthy adults based on single timepoint data. Here, we analysed the within-host evolution of 82 longitudinally-sampled individuals, mostly young children, infected with A/H1N1pdm09 or A/H3N2 viruses between 2007 and 2009. For A/H1N1pdm09 infections during the 2009 pandemic, nonsynonymous minority variants were more prevalent than synonymous ones. For A/H3N2 viruses in young children, early infection was dominated by purifying selection. As these infections progressed, nonsynonymous variants typically increased in frequency even when within-host virus titres decreased. Unlike the short-lived infections of adults where de novo within-host variants are rare, longer infections in young children allow for the maintenance of virus diversity via mutation-selection balance creating potentially important opportunities for within-host virus evolution.
UR - https://www.scopus.com/pages/publications/85112443339
U2 - 10.7554/ELIFE.68917
DO - 10.7554/ELIFE.68917
M3 - Article
C2 - 34342576
SN - 2050-084X
VL - 10
JO - eLife
JF - eLife
ER -
SDG 3 Good Health and Well-being