Vagal activation in novelty-induced tachycardia during the light phase in the rat

The effects of repeated exposure to a novel test box on cardiac and behavioral activities (locomotion, rearing, grooming, scanning, and immobility) were studied in rats rested during the dark phase ('dark' rats) or the light phase ('light' rats) of the lighting cycle, using a telemetry system for registration of ECGs during the first and fifth tests. Heart rate (HR) was used to monitor sympathetic and parasympathetic activity; the PQ interval was used to monitor parasympathetic activity. Behavior was videotaped simultaneously. In light rats, the first and fifth exposures to the test box resulted in higher increases of active behavior and HR than in dark rats, whereas the duration of the PQ interval of the ECG was increased in light rats only. This indicates that in the light phase novelty induces active behavior associated with an increase in both sympathetic and vagal outflow, whereas in the dark phase behavioral activation is predominantly associated with increased sympathetic activity, without appreciable changes in vagal outflow. In addition, light rats showed less active behavior during the fifth than during the first exposure, indicating behavioral habituation. This behavioral habituation to the test box in the light phase coincided with vagal habituation (a diminution of the PQ interval). The increase of the tachycardiac response during the fifth exposure as compared to the first exposure suggests that it is not likely that sympathetic outflow was part of the habituation process. In dark rats no behavioral or cardiac habituation was found.