Upregulated Retinal Neurofilament Expression in Experimental Optic Neuritis

Robert Weissert; Therése Hugosson; Axel Petzold

doi:10.1080/01658107.2022.2025852

Upregulated Retinal Neurofilament Expression in Experimental Optic Neuritis

Robert Weissert
, Therése Hugosson
, Axel Petzold^*

^*Corresponding author for this work

University Hospital Regensburg
Karolinska Institutet
Lund University
University College London

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

In optic neuritis (ON), transient thickening of the macular retinal nerve fibre layer (RNFL) can be observed. This optical coherence tomography-based observation is not understood. The axonal diameter correlates with the neurofilament (Nf) protein content, but there are no data on the retinal tissue concentration of Nfs. The myelin-oligodendrocyte-glycoprotein (MOG) induced experimental autoimmune encephalomyelitis (EAE) model was used to investigate the retinas of Brown Norway rats with (i) visual evoked potentials (VEP) confirmed ON, (ii) VEP confirmed absence of ON and (iii) control animals. Twenty retinas were collected from MOG-EAE and control rats 27 days after immunisation. Retinal tissue Nf concentrations per total protein (μg/mg) were significantly higher in MOG-EAE rats with ON (median 4.29, interquartile range [IQR] 3.41–5.97) compared with MOG-EAE rats without ON (1.14, IQR 1.10–1.67) or control rats (0.93, IQR 0.45–4.00). The data suggest that up-regulation of Nf expression in the retinal ganglion cells precedes development of RNFL atrophy and plausibly explains the transient increase of axonal diameter and RNFL thickening.

Original language	English
Pages (from-to)	215-219
Number of pages	5
Journal	NEURO-OPHTHALMOLOGY
Volume	46
Issue number	4
Early online date	2022
DOIs	https://doi.org/10.1080/01658107.2022.2025852
Publication status	Published - 2022

Keywords

Optic neuritis
axonal degeneration
neurofilament protein
retina
tissue

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10.1080/01658107.2022.2025852

Upregulated-retinal-neurofilament-expression-in-experimental-optic-neuritis.pdfFinal published version, 659 KBLicence: Taverne

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title = "Upregulated Retinal Neurofilament Expression in Experimental Optic Neuritis",

abstract = "In optic neuritis (ON), transient thickening of the macular retinal nerve fibre layer (RNFL) can be observed. This optical coherence tomography-based observation is not understood. The axonal diameter correlates with the neurofilament (Nf) protein content, but there are no data on the retinal tissue concentration of Nfs. The myelin-oligodendrocyte-glycoprotein (MOG) induced experimental autoimmune encephalomyelitis (EAE) model was used to investigate the retinas of Brown Norway rats with (i) visual evoked potentials (VEP) confirmed ON, (ii) VEP confirmed absence of ON and (iii) control animals. Twenty retinas were collected from MOG-EAE and control rats 27 days after immunisation. Retinal tissue Nf concentrations per total protein (μg/mg) were significantly higher in MOG-EAE rats with ON (median 4.29, interquartile range [IQR] 3.41–5.97) compared with MOG-EAE rats without ON (1.14, IQR 1.10–1.67) or control rats (0.93, IQR 0.45–4.00). The data suggest that up-regulation of Nf expression in the retinal ganglion cells precedes development of RNFL atrophy and plausibly explains the transient increase of axonal diameter and RNFL thickening.",

keywords = "Optic neuritis, axonal degeneration, neurofilament protein, retina, tissue",

author = "Robert Weissert and Ther{\'e}se Hugosson and Axel Petzold",

note = "Funding Information: This study was supported by a grant of the Deutsche Forschungsgemeinschaft (DfG) to RW. AP is supported by the National Institute for Health Research (NIHR) Biomedical Research Centre based at Moorfields Eye Hospital NHS Foundation Trust and UCL Institute of Ophthalmology. Publisher Copyright: {\textcopyright} 2022 Taylor \& Francis Group, LLC.",

year = "2022",

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T1 - Upregulated Retinal Neurofilament Expression in Experimental Optic Neuritis

AU - Weissert, Robert

AU - Hugosson, Therése

AU - Petzold, Axel

N1 - Funding Information: This study was supported by a grant of the Deutsche Forschungsgemeinschaft (DfG) to RW. AP is supported by the National Institute for Health Research (NIHR) Biomedical Research Centre based at Moorfields Eye Hospital NHS Foundation Trust and UCL Institute of Ophthalmology. Publisher Copyright: © 2022 Taylor & Francis Group, LLC.

PY - 2022

Y1 - 2022

N2 - In optic neuritis (ON), transient thickening of the macular retinal nerve fibre layer (RNFL) can be observed. This optical coherence tomography-based observation is not understood. The axonal diameter correlates with the neurofilament (Nf) protein content, but there are no data on the retinal tissue concentration of Nfs. The myelin-oligodendrocyte-glycoprotein (MOG) induced experimental autoimmune encephalomyelitis (EAE) model was used to investigate the retinas of Brown Norway rats with (i) visual evoked potentials (VEP) confirmed ON, (ii) VEP confirmed absence of ON and (iii) control animals. Twenty retinas were collected from MOG-EAE and control rats 27 days after immunisation. Retinal tissue Nf concentrations per total protein (μg/mg) were significantly higher in MOG-EAE rats with ON (median 4.29, interquartile range [IQR] 3.41–5.97) compared with MOG-EAE rats without ON (1.14, IQR 1.10–1.67) or control rats (0.93, IQR 0.45–4.00). The data suggest that up-regulation of Nf expression in the retinal ganglion cells precedes development of RNFL atrophy and plausibly explains the transient increase of axonal diameter and RNFL thickening.

AB - In optic neuritis (ON), transient thickening of the macular retinal nerve fibre layer (RNFL) can be observed. This optical coherence tomography-based observation is not understood. The axonal diameter correlates with the neurofilament (Nf) protein content, but there are no data on the retinal tissue concentration of Nfs. The myelin-oligodendrocyte-glycoprotein (MOG) induced experimental autoimmune encephalomyelitis (EAE) model was used to investigate the retinas of Brown Norway rats with (i) visual evoked potentials (VEP) confirmed ON, (ii) VEP confirmed absence of ON and (iii) control animals. Twenty retinas were collected from MOG-EAE and control rats 27 days after immunisation. Retinal tissue Nf concentrations per total protein (μg/mg) were significantly higher in MOG-EAE rats with ON (median 4.29, interquartile range [IQR] 3.41–5.97) compared with MOG-EAE rats without ON (1.14, IQR 1.10–1.67) or control rats (0.93, IQR 0.45–4.00). The data suggest that up-regulation of Nf expression in the retinal ganglion cells precedes development of RNFL atrophy and plausibly explains the transient increase of axonal diameter and RNFL thickening.

KW - Optic neuritis

KW - axonal degeneration

KW - neurofilament protein

KW - retina

KW - tissue

UR - https://www.scopus.com/pages/publications/85124070323

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DO - 10.1080/01658107.2022.2025852

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SP - 215

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JO - NEURO-OPHTHALMOLOGY

JF - NEURO-OPHTHALMOLOGY

IS - 4

ER -

Upregulated Retinal Neurofilament Expression in Experimental Optic Neuritis

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