Untargeted Molecular Analysis of Exhaled Breath as a Diagnostic Test for Ventilator-Associated Lower Respiratory Tract Infections (BreathDx)

Pouline M. P. van Oort; Tamara M. Nijsen; Iain R. White; Hugo H. Knobel; Timothy Felton; Nicholas Rattray; Oluwasola Lawal; Murtaza Bulut; Waqar Ahmed; Antonio Artigas; Pedro R. Povoa; Ignacio Martin-Loeches; Hans Weda; Royston Goodacre; Marcus J. Schultz; Paul M. Dark; Stephen J. Fowler; Lieuwe D. Bos

doi:10.1136/thoraxjnl-2021-217362

Untargeted Molecular Analysis of Exhaled Breath as a Diagnostic Test for Ventilator-Associated Lower Respiratory Tract Infections (BreathDx)

Pouline M. P. van Oort
, Tamara M. Nijsen
, Iain R. White
, Hugo H. Knobel
, Timothy Felton
, Nicholas Rattray
, Oluwasola Lawal
, Murtaza Bulut
, Waqar Ahmed
, Antonio Artigas
, Pedro R. Povoa
, Ignacio Martin-Loeches
, Hans Weda
, Royston Goodacre
, Marcus J. Schultz
, Paul M. Dark
, Stephen J. Fowler
, Lieuwe D. Bos^*

^*Corresponding author for this work

Amsterdam UMC - University of Amsterdam
Koninklijke Philips N.V.
University of Nova Gorica
Division of Infection, Immunity and Respiratory Medicine, University of Manchester, Manchester, UK
EurofinsEAG, Eurofins Materials Science Netherlands B.V., Eindhoven, The Netherlands
University of Strathclyde
University of Manchester
Autonomous University of Barcelona
Intensive Care, Centro Hospitalar de Lisboa Central, Lisbon, Portugal
Trinity College Dublin
Orikami, Nijmegen, The Netherlands
University of Liverpool
Mahidol University
Lancashire Teaching Hospitals NHS Foundation Trust
Amsterdam University Medical Centers
Centro Hospitalar de Lisboa Central
St James's Hospital
Orikami
Wythenshawe Hospital

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Patients suspected of ventilator-associated lower respiratory tract infections (VA-LRTIs) commonly receive broad-spectrum antimicrobial therapy unnecessarily. We tested whether exhaled breath analysis can discriminate between patients suspected of VA-LRTI with confirmed infection, from patients with negative cultures. Breath from 108 patients suspected of VA-LRTI was analysed by gas chromatography-mass spectrometry. The breath test had a sensitivity of 98% at a specificity of 49%, confirmed with a second analytical method. The breath test had a negative predictive value of 96% and excluded pneumonia in half of the patients with negative cultures. Trial registration number: UKCRN ID number 19086, registered May 2015.

Original language	English
Article number	217362
Pages (from-to)	79-81
Number of pages	3
Journal	Thorax
Volume	77
Issue number	1
Early online date	2021
DOIs	https://doi.org/10.1136/thoraxjnl-2021-217362
Publication status	Published - 1 Jan 2022

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

assisted ventilation
critical care
pneumonia

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10.1136/thoraxjnl-2021-217362

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van Oort, P. M. P., Nijsen, T. M., White, I. R., Knobel, H. H., Felton, T., Rattray, N., Lawal, O., Bulut, M., Ahmed, W., Artigas, A., Povoa, P. R., Martin-Loeches, I., Weda, H., Goodacre, R., Schultz, M. J., Dark, P. M., Fowler, S. J., & Bos, L. D. (2022). Untargeted Molecular Analysis of Exhaled Breath as a Diagnostic Test for Ventilator-Associated Lower Respiratory Tract Infections (BreathDx). Thorax, 77(1), 79-81. Article 217362. https://doi.org/10.1136/thoraxjnl-2021-217362

@article{0baf219ac04d44499d840d7baf4b5e54,

title = "Untargeted Molecular Analysis of Exhaled Breath as a Diagnostic Test for Ventilator-Associated Lower Respiratory Tract Infections (BreathDx)",

abstract = "Patients suspected of ventilator-associated lower respiratory tract infections (VA-LRTIs) commonly receive broad-spectrum antimicrobial therapy unnecessarily. We tested whether exhaled breath analysis can discriminate between patients suspected of VA-LRTI with confirmed infection, from patients with negative cultures. Breath from 108 patients suspected of VA-LRTI was analysed by gas chromatography-mass spectrometry. The breath test had a sensitivity of 98\% at a specificity of 49\%, confirmed with a second analytical method. The breath test had a negative predictive value of 96\% and excluded pneumonia in half of the patients with negative cultures. Trial registration number: UKCRN ID number 19086, registered May 2015.",

keywords = "assisted ventilation, critical care, pneumonia",

author = "\{van Oort\}, \{Pouline M. P.\} and Nijsen, \{Tamara M.\} and White, \{Iain R.\} and Knobel, \{Hugo H.\} and Timothy Felton and Nicholas Rattray and Oluwasola Lawal and Murtaza Bulut and Waqar Ahmed and Antonio Artigas and Povoa, \{Pedro R.\} and Ignacio Martin-Loeches and Hans Weda and Royston Goodacre and Schultz, \{Marcus J.\} and Dark, \{Paul M.\} and Fowler, \{Stephen J.\} and Bos, \{Lieuwe D.\}",

note = "Funding Information: Funding The project was funded by the European Union FP7 Marie Curie Actions, under the Industry-Academia Partnerships and Pathways (IAPP) programme (MC-IAPP BreathDx 611951). IRW, TF, PMD and SJF were supported by the NIHR Manchester Biochemical Research Centre. Competing interests None declared. Patient consent for publication Not required. Publisher Copyright: {\textcopyright}",

year = "2022",

month = jan,

day = "1",

doi = "10.1136/thoraxjnl-2021-217362",

language = "English",

volume = "77",

pages = "79--81",

journal = "Thorax",

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van Oort, PMP, Nijsen, TM, White, IR, Knobel, HH, Felton, T, Rattray, N, Lawal, O, Bulut, M, Ahmed, W, Artigas, A, Povoa, PR, Martin-Loeches, I, Weda, H, Goodacre, R, Schultz, MJ, Dark, PM, Fowler, SJ & Bos, LD 2022, 'Untargeted Molecular Analysis of Exhaled Breath as a Diagnostic Test for Ventilator-Associated Lower Respiratory Tract Infections (BreathDx)', Thorax, vol. 77, no. 1, 217362, pp. 79-81. https://doi.org/10.1136/thoraxjnl-2021-217362

TY - JOUR

T1 - Untargeted Molecular Analysis of Exhaled Breath as a Diagnostic Test for Ventilator-Associated Lower Respiratory Tract Infections (BreathDx)

AU - van Oort, Pouline M. P.

AU - Nijsen, Tamara M.

AU - White, Iain R.

AU - Knobel, Hugo H.

AU - Felton, Timothy

AU - Rattray, Nicholas

AU - Lawal, Oluwasola

AU - Bulut, Murtaza

AU - Ahmed, Waqar

AU - Artigas, Antonio

AU - Povoa, Pedro R.

AU - Martin-Loeches, Ignacio

AU - Weda, Hans

AU - Goodacre, Royston

AU - Schultz, Marcus J.

AU - Dark, Paul M.

AU - Fowler, Stephen J.

AU - Bos, Lieuwe D.

N1 - Funding Information: Funding The project was funded by the European Union FP7 Marie Curie Actions, under the Industry-Academia Partnerships and Pathways (IAPP) programme (MC-IAPP BreathDx 611951). IRW, TF, PMD and SJF were supported by the NIHR Manchester Biochemical Research Centre. Competing interests None declared. Patient consent for publication Not required. Publisher Copyright: ©

PY - 2022/1/1

Y1 - 2022/1/1

N2 - Patients suspected of ventilator-associated lower respiratory tract infections (VA-LRTIs) commonly receive broad-spectrum antimicrobial therapy unnecessarily. We tested whether exhaled breath analysis can discriminate between patients suspected of VA-LRTI with confirmed infection, from patients with negative cultures. Breath from 108 patients suspected of VA-LRTI was analysed by gas chromatography-mass spectrometry. The breath test had a sensitivity of 98% at a specificity of 49%, confirmed with a second analytical method. The breath test had a negative predictive value of 96% and excluded pneumonia in half of the patients with negative cultures. Trial registration number: UKCRN ID number 19086, registered May 2015.

AB - Patients suspected of ventilator-associated lower respiratory tract infections (VA-LRTIs) commonly receive broad-spectrum antimicrobial therapy unnecessarily. We tested whether exhaled breath analysis can discriminate between patients suspected of VA-LRTI with confirmed infection, from patients with negative cultures. Breath from 108 patients suspected of VA-LRTI was analysed by gas chromatography-mass spectrometry. The breath test had a sensitivity of 98% at a specificity of 49%, confirmed with a second analytical method. The breath test had a negative predictive value of 96% and excluded pneumonia in half of the patients with negative cultures. Trial registration number: UKCRN ID number 19086, registered May 2015.

KW - assisted ventilation

KW - critical care

KW - pneumonia

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UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85107683532&origin=inward

UR - https://www.ncbi.nlm.nih.gov/pubmed/34088787

U2 - 10.1136/thoraxjnl-2021-217362

DO - 10.1136/thoraxjnl-2021-217362

M3 - Article

C2 - 34088787

SN - 0040-6376

VL - 77

SP - 79

EP - 81

JO - Thorax

JF - Thorax

IS - 1

M1 - 217362

ER -

Untargeted Molecular Analysis of Exhaled Breath as a Diagnostic Test for Ventilator-Associated Lower Respiratory Tract Infections (BreathDx)

Abstract

UN SDGs

Keywords

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