The sensitivity of quiescent and proliferating mouse spermatogonial stem cells to X irradiation

Y. van der Meer; R. Huiskamp; J. A. Davids; I. van der Tweel; D. G. de rooij

doi:10.2307/3578373

The sensitivity of quiescent and proliferating mouse spermatogonial stem cells to X irradiation

Y. van der Meer
, R. Huiskamp
, J. A. Davids
, I. van der Tweel
, D. G. de rooij

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The radiosensitivity of spermatogonial stem cells to X rays was determined in the various stages of the cycle of the seminiferous epithelium of the CBA mouse. The numbers of undifferentiated spermatogonia present 10 days after graded doses of X rays (0.5-8.0 Gy) were taken as a measure of stem cell survival. Dose-response relationships were generated for each stage of the epithelial cycle by counting spermatogonial numbers and also by using the repopulation index method. Spermatogonial stem cells were found to be most sensitive to X rays during quiescence (stages IV-VII) and most resistant during active proliferation (stages IX-II). The D0 for X rays varied from 1.0 Gy for quiescent spermatogonial stem cells to 2.4 Gy for actively proliferating stem cells. In most epithelial stages the dose-response curves showed no shoulder in the low-dose region

Original language	English
Pages (from-to)	289-295
Journal	Radiation research
Volume	130
Issue number	3
DOIs	https://doi.org/10.2307/3578373
Publication status	Published - 1992

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title = "The sensitivity of quiescent and proliferating mouse spermatogonial stem cells to X irradiation",

abstract = "The radiosensitivity of spermatogonial stem cells to X rays was determined in the various stages of the cycle of the seminiferous epithelium of the CBA mouse. The numbers of undifferentiated spermatogonia present 10 days after graded doses of X rays (0.5-8.0 Gy) were taken as a measure of stem cell survival. Dose-response relationships were generated for each stage of the epithelial cycle by counting spermatogonial numbers and also by using the repopulation index method. Spermatogonial stem cells were found to be most sensitive to X rays during quiescence (stages IV-VII) and most resistant during active proliferation (stages IX-II). The D0 for X rays varied from 1.0 Gy for quiescent spermatogonial stem cells to 2.4 Gy for actively proliferating stem cells. In most epithelial stages the dose-response curves showed no shoulder in the low-dose region",

author = "\{van der Meer\}, Y. and R. Huiskamp and Davids, \{J. A.\} and \{van der Tweel\}, I. and \{de rooij\}, \{D. G.\}",

year = "1992",

doi = "10.2307/3578373",

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journal = "Radiation research",

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T1 - The sensitivity of quiescent and proliferating mouse spermatogonial stem cells to X irradiation

AU - van der Meer, Y.

AU - Huiskamp, R.

AU - Davids, J. A.

AU - van der Tweel, I.

AU - de rooij, D. G.

PY - 1992

Y1 - 1992

N2 - The radiosensitivity of spermatogonial stem cells to X rays was determined in the various stages of the cycle of the seminiferous epithelium of the CBA mouse. The numbers of undifferentiated spermatogonia present 10 days after graded doses of X rays (0.5-8.0 Gy) were taken as a measure of stem cell survival. Dose-response relationships were generated for each stage of the epithelial cycle by counting spermatogonial numbers and also by using the repopulation index method. Spermatogonial stem cells were found to be most sensitive to X rays during quiescence (stages IV-VII) and most resistant during active proliferation (stages IX-II). The D0 for X rays varied from 1.0 Gy for quiescent spermatogonial stem cells to 2.4 Gy for actively proliferating stem cells. In most epithelial stages the dose-response curves showed no shoulder in the low-dose region

AB - The radiosensitivity of spermatogonial stem cells to X rays was determined in the various stages of the cycle of the seminiferous epithelium of the CBA mouse. The numbers of undifferentiated spermatogonia present 10 days after graded doses of X rays (0.5-8.0 Gy) were taken as a measure of stem cell survival. Dose-response relationships were generated for each stage of the epithelial cycle by counting spermatogonial numbers and also by using the repopulation index method. Spermatogonial stem cells were found to be most sensitive to X rays during quiescence (stages IV-VII) and most resistant during active proliferation (stages IX-II). The D0 for X rays varied from 1.0 Gy for quiescent spermatogonial stem cells to 2.4 Gy for actively proliferating stem cells. In most epithelial stages the dose-response curves showed no shoulder in the low-dose region

U2 - 10.2307/3578373

DO - 10.2307/3578373

M3 - Article

C2 - 1594754

SN - 0033-7587

VL - 130

SP - 289

EP - 295

JO - Radiation research

JF - Radiation research

IS - 3

ER -

The sensitivity of quiescent and proliferating mouse spermatogonial stem cells to X irradiation

Abstract

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