The region Ser333-Arg356 of the alpha-chain of human C4b-binding protein is involved in the binding of complement C4b

M. Hessing; D. Kanters; H. Takeya; C. van 't Veer; T. M. Hackeng; S. Iwanaga; B. N. Bouma

doi:10.1016/0014-5793(93)81281-4

The region Ser333-Arg356 of the alpha-chain of human C4b-binding protein is involved in the binding of complement C4b

M. Hessing
, D. Kanters
, H. Takeya
, C. van 't Veer
, T. M. Hackeng
, S. Iwanaga
, B. N. Bouma

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Human C4b-binding protein (C4BP) functions as a cofactor to factor I in the degradation of C4b and accelerates the decay rate of the C4b2a complex. In this study we describe a monoclonal antibody directed against the alpha-chain of C4BP that inhibits the binding of C4b to C4BP. In order to identify the structural domain of the alpha-chain of C4BP that interacts with C4b, tryptic fragments of C4BP were generated. Amino acid sequence analysis of the fragments revealed that the residues Ser333-Arg356 of the alpha-chain of C4BP contain the epitope of this antibody, and as a consequence, that this part of the alpha-chain of C4BP is likely to be involved in the interaction with C4b

Original language	English
Pages (from-to)	228-232
Journal	FEBS letters
Volume	317
Issue number	3
DOIs	https://doi.org/10.1016/0014-5793(93)81281-4
Publication status	Published - 1993

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10.1016/0014-5793(93)81281-4

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title = "The region Ser333-Arg356 of the alpha-chain of human C4b-binding protein is involved in the binding of complement C4b",

abstract = "Human C4b-binding protein (C4BP) functions as a cofactor to factor I in the degradation of C4b and accelerates the decay rate of the C4b2a complex. In this study we describe a monoclonal antibody directed against the alpha-chain of C4BP that inhibits the binding of C4b to C4BP. In order to identify the structural domain of the alpha-chain of C4BP that interacts with C4b, tryptic fragments of C4BP were generated. Amino acid sequence analysis of the fragments revealed that the residues Ser333-Arg356 of the alpha-chain of C4BP contain the epitope of this antibody, and as a consequence, that this part of the alpha-chain of C4BP is likely to be involved in the interaction with C4b",

author = "M. Hessing and D. Kanters and H. Takeya and \{van 't Veer\}, C. and Hackeng, \{T. M.\} and S. Iwanaga and Bouma, \{B. N.\}",

year = "1993",

doi = "10.1016/0014-5793(93)81281-4",

language = "English",

volume = "317",

pages = "228--232",

journal = "FEBS letters",

issn = "0014-5793",

publisher = "Wiley Blackwell",

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TY - JOUR

T1 - The region Ser333-Arg356 of the alpha-chain of human C4b-binding protein is involved in the binding of complement C4b

AU - Hessing, M.

AU - Kanters, D.

AU - Takeya, H.

AU - van 't Veer, C.

AU - Hackeng, T. M.

AU - Iwanaga, S.

AU - Bouma, B. N.

PY - 1993

Y1 - 1993

N2 - Human C4b-binding protein (C4BP) functions as a cofactor to factor I in the degradation of C4b and accelerates the decay rate of the C4b2a complex. In this study we describe a monoclonal antibody directed against the alpha-chain of C4BP that inhibits the binding of C4b to C4BP. In order to identify the structural domain of the alpha-chain of C4BP that interacts with C4b, tryptic fragments of C4BP were generated. Amino acid sequence analysis of the fragments revealed that the residues Ser333-Arg356 of the alpha-chain of C4BP contain the epitope of this antibody, and as a consequence, that this part of the alpha-chain of C4BP is likely to be involved in the interaction with C4b

AB - Human C4b-binding protein (C4BP) functions as a cofactor to factor I in the degradation of C4b and accelerates the decay rate of the C4b2a complex. In this study we describe a monoclonal antibody directed against the alpha-chain of C4BP that inhibits the binding of C4b to C4BP. In order to identify the structural domain of the alpha-chain of C4BP that interacts with C4b, tryptic fragments of C4BP were generated. Amino acid sequence analysis of the fragments revealed that the residues Ser333-Arg356 of the alpha-chain of C4BP contain the epitope of this antibody, and as a consequence, that this part of the alpha-chain of C4BP is likely to be involved in the interaction with C4b

U2 - 10.1016/0014-5793(93)81281-4

DO - 10.1016/0014-5793(93)81281-4

M3 - Article

C2 - 8425609

SN - 0014-5793

VL - 317

SP - 228

EP - 232

JO - FEBS letters

JF - FEBS letters

IS - 3

ER -

The region Ser333-Arg356 of the alpha-chain of human C4b-binding protein is involved in the binding of complement C4b

Abstract

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