The influence of cytokines on the integrity of the blood-brain barrier in vitro

Helga E. De Vries; Margret C.M. Blom-Roosemalen; Marijke Van Oosten; Albert G. De Boer; Theo J.C. Van Berkel; Douwe D. Breimer; Johan Kuiper

doi:10.1016/0165-5728(95)00148-4

The influence of cytokines on the integrity of the blood-brain barrier in vitro

Helga E. De Vries^*
, Margret C.M. Blom-Roosemalen
, Marijke Van Oosten
, Albert G. De Boer
, Theo J.C. Van Berkel
, Douwe D. Breimer
, Johan Kuiper

^*Corresponding author for this work

Leiden University

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The effects of the cytokines tumour necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6 on the permeability of monolayers of rat cerebral endothelial cells (RCEC) were investigated to assess potential changes in the integrity of the blood-brain barrier (BBB). RCEC were cultured to tight monolayers with a trans endothelial electrical resistance (TEER) of 100-150 Ω · cm² on polycarbonate filters. Exposure of the RCEC to TNF-α, IL-1β and IL-6 induced a decline in the TEER, which could be completely abolished by 1 μM of indomethacin, a cyclooxygenase inhibitor. In addition, the effect of IL-1β on TEER across monolayers of RCEC could be completely inhibited by IL-1 receptor antagonist. In conclusion, cytokines induce a disruption of the BBB in vitro. In this process, cyclooxygenase activation within the endothelial cells seems to play a key role.

Original language	English
Pages (from-to)	37-43
Number of pages	7
Journal	Journal of neuroimmunology
Volume	64
Issue number	1
DOIs	https://doi.org/10.1016/0165-5728(95)00148-4
Publication status	Published - 1996

Keywords

Blood-brain barrier
Cerebral endothelial cells
Cytokines
Inflammation

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10.1016/0165-5728(95)00148-4

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@article{67caeed0e02145bd8916be7e8e529423,

title = "The influence of cytokines on the integrity of the blood-brain barrier in vitro",

abstract = "The effects of the cytokines tumour necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6 on the permeability of monolayers of rat cerebral endothelial cells (RCEC) were investigated to assess potential changes in the integrity of the blood-brain barrier (BBB). RCEC were cultured to tight monolayers with a trans endothelial electrical resistance (TEER) of 100-150 Ω · cm2 on polycarbonate filters. Exposure of the RCEC to TNF-α, IL-1β and IL-6 induced a decline in the TEER, which could be completely abolished by 1 μM of indomethacin, a cyclooxygenase inhibitor. In addition, the effect of IL-1β on TEER across monolayers of RCEC could be completely inhibited by IL-1 receptor antagonist. In conclusion, cytokines induce a disruption of the BBB in vitro. In this process, cyclooxygenase activation within the endothelial cells seems to play a key role.",

keywords = "Blood-brain barrier, Cerebral endothelial cells, Cytokines, Inflammation",

author = "\{De Vries\}, \{Helga E.\} and Blom-Roosemalen, \{Margret C.M.\} and \{Van Oosten\}, Marijke and \{De Boer\}, \{Albert G.\} and \{Van Berkel\}, \{Theo J.C.\} and Breimer, \{Douwe D.\} and Johan Kuiper",

year = "1996",

doi = "10.1016/0165-5728(95)00148-4",

language = "English",

volume = "64",

pages = "37--43",

journal = "Journal of neuroimmunology",

issn = "0165-5728",

publisher = "Elsevier",

number = "1",

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TY - JOUR

T1 - The influence of cytokines on the integrity of the blood-brain barrier in vitro

AU - De Vries, Helga E.

AU - Blom-Roosemalen, Margret C.M.

AU - Van Oosten, Marijke

AU - De Boer, Albert G.

AU - Van Berkel, Theo J.C.

AU - Breimer, Douwe D.

AU - Kuiper, Johan

PY - 1996

Y1 - 1996

N2 - The effects of the cytokines tumour necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6 on the permeability of monolayers of rat cerebral endothelial cells (RCEC) were investigated to assess potential changes in the integrity of the blood-brain barrier (BBB). RCEC were cultured to tight monolayers with a trans endothelial electrical resistance (TEER) of 100-150 Ω · cm2 on polycarbonate filters. Exposure of the RCEC to TNF-α, IL-1β and IL-6 induced a decline in the TEER, which could be completely abolished by 1 μM of indomethacin, a cyclooxygenase inhibitor. In addition, the effect of IL-1β on TEER across monolayers of RCEC could be completely inhibited by IL-1 receptor antagonist. In conclusion, cytokines induce a disruption of the BBB in vitro. In this process, cyclooxygenase activation within the endothelial cells seems to play a key role.

AB - The effects of the cytokines tumour necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6 on the permeability of monolayers of rat cerebral endothelial cells (RCEC) were investigated to assess potential changes in the integrity of the blood-brain barrier (BBB). RCEC were cultured to tight monolayers with a trans endothelial electrical resistance (TEER) of 100-150 Ω · cm2 on polycarbonate filters. Exposure of the RCEC to TNF-α, IL-1β and IL-6 induced a decline in the TEER, which could be completely abolished by 1 μM of indomethacin, a cyclooxygenase inhibitor. In addition, the effect of IL-1β on TEER across monolayers of RCEC could be completely inhibited by IL-1 receptor antagonist. In conclusion, cytokines induce a disruption of the BBB in vitro. In this process, cyclooxygenase activation within the endothelial cells seems to play a key role.

KW - Blood-brain barrier

KW - Cerebral endothelial cells

KW - Cytokines

KW - Inflammation

UR - https://www.scopus.com/pages/publications/0030034301

U2 - 10.1016/0165-5728(95)00148-4

DO - 10.1016/0165-5728(95)00148-4

M3 - Article

C2 - 8598388

SN - 0165-5728

VL - 64

SP - 37

EP - 43

JO - Journal of neuroimmunology

JF - Journal of neuroimmunology

IS - 1

ER -

The influence of cytokines on the integrity of the blood-brain barrier in vitro

Abstract

Keywords

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