The effect of cyclopentenyl cytosine on human SK-N-BE(2)-C neuroblastoma cells

R. J. Slingerland; A. H. van Gennip; J. M. Bodlaender; P. A. Voûte; A. B. van Kuilenburg

doi:10.1016/0006-2952(95)00024-T

The effect of cyclopentenyl cytosine on human SK-N-BE(2)-C neuroblastoma cells

R. J. Slingerland
, A. H. van Gennip
, J. M. Bodlaender
, P. A. Voûte
, A. B. van Kuilenburg

Research output: Contribution to journal › Comment/Letter to the editor › Academic

Abstract

Human neuroblastoma SK-N-BE(2)-C cell-line cells were cultured in the presence of various concentrations of cyclopentenyl cytosine (CPEC). In the absence of cytidine, the IC50 value of CPEC for SK-N-BE(2)-C cells was 100 nM after 72 hr drug exposure. The IC20 value was 1 microM after 24 hr of exposure to CPEC in the presence of 10 microM cytidine, whereas in the absence of cytidine, CPEC at 1 microM resulted in an IC40 value after 24 hr. Therefore, cytidine partially prevented the cytostatic effect of CPEC. Cells cultured with 1 microM CPEC for 72 hr were enriched by approximately 410% with mono- and oligonucleosomes in comparison with cells cultured without CPEC. This enrichment was partially prevented with 10 microM deoxycytidine and completely prevented with 10 microM cytidine

Original language	English
Pages (from-to)	277-279
Journal	Biochemical pharmacology
Volume	50
Issue number	2
DOIs	https://doi.org/10.1016/0006-2952(95)00024-T
Publication status	Published - 1995

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10.1016/0006-2952(95)00024-T

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@article{ef5f9738e4c24990aef614b2a71b107a,

title = "The effect of cyclopentenyl cytosine on human SK-N-BE(2)-C neuroblastoma cells",

abstract = "Human neuroblastoma SK-N-BE(2)-C cell-line cells were cultured in the presence of various concentrations of cyclopentenyl cytosine (CPEC). In the absence of cytidine, the IC50 value of CPEC for SK-N-BE(2)-C cells was 100 nM after 72 hr drug exposure. The IC20 value was 1 microM after 24 hr of exposure to CPEC in the presence of 10 microM cytidine, whereas in the absence of cytidine, CPEC at 1 microM resulted in an IC40 value after 24 hr. Therefore, cytidine partially prevented the cytostatic effect of CPEC. Cells cultured with 1 microM CPEC for 72 hr were enriched by approximately 410\% with mono- and oligonucleosomes in comparison with cells cultured without CPEC. This enrichment was partially prevented with 10 microM deoxycytidine and completely prevented with 10 microM cytidine",

author = "Slingerland, \{R. J.\} and \{van Gennip\}, \{A. H.\} and Bodlaender, \{J. M.\} and Vo{\^u}te, \{P. A.\} and \{van Kuilenburg\}, \{A. B.\}",

year = "1995",

doi = "10.1016/0006-2952(95)00024-T",

language = "English",

volume = "50",

pages = "277--279",

journal = "Biochemical pharmacology",

issn = "0006-2952",

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TY - JOUR

T1 - The effect of cyclopentenyl cytosine on human SK-N-BE(2)-C neuroblastoma cells

AU - Slingerland, R. J.

AU - van Gennip, A. H.

AU - Bodlaender, J. M.

AU - Voûte, P. A.

AU - van Kuilenburg, A. B.

PY - 1995

Y1 - 1995

N2 - Human neuroblastoma SK-N-BE(2)-C cell-line cells were cultured in the presence of various concentrations of cyclopentenyl cytosine (CPEC). In the absence of cytidine, the IC50 value of CPEC for SK-N-BE(2)-C cells was 100 nM after 72 hr drug exposure. The IC20 value was 1 microM after 24 hr of exposure to CPEC in the presence of 10 microM cytidine, whereas in the absence of cytidine, CPEC at 1 microM resulted in an IC40 value after 24 hr. Therefore, cytidine partially prevented the cytostatic effect of CPEC. Cells cultured with 1 microM CPEC for 72 hr were enriched by approximately 410% with mono- and oligonucleosomes in comparison with cells cultured without CPEC. This enrichment was partially prevented with 10 microM deoxycytidine and completely prevented with 10 microM cytidine

AB - Human neuroblastoma SK-N-BE(2)-C cell-line cells were cultured in the presence of various concentrations of cyclopentenyl cytosine (CPEC). In the absence of cytidine, the IC50 value of CPEC for SK-N-BE(2)-C cells was 100 nM after 72 hr drug exposure. The IC20 value was 1 microM after 24 hr of exposure to CPEC in the presence of 10 microM cytidine, whereas in the absence of cytidine, CPEC at 1 microM resulted in an IC40 value after 24 hr. Therefore, cytidine partially prevented the cytostatic effect of CPEC. Cells cultured with 1 microM CPEC for 72 hr were enriched by approximately 410% with mono- and oligonucleosomes in comparison with cells cultured without CPEC. This enrichment was partially prevented with 10 microM deoxycytidine and completely prevented with 10 microM cytidine

U2 - 10.1016/0006-2952(95)00024-T

DO - 10.1016/0006-2952(95)00024-T

M3 - Comment/Letter to the editor

C2 - 7632174

SN - 0006-2952

VL - 50

SP - 277

EP - 279

JO - Biochemical pharmacology

JF - Biochemical pharmacology

IS - 2

ER -

The effect of cyclopentenyl cytosine on human SK-N-BE(2)-C neuroblastoma cells

Abstract

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