Synthesis and evaluation of a fluorine-18 labeled antisense oligonucleotide as a potential PET tracer for iNOS mRNA expression

Erik F. J. de Vries; Joke Vroegh; Gerard Dijkstra; Han Moshage; Philip H. Elsinga; Peter L. M. Jansen; Willem Vaalburg

doi:10.1016/j.nucmedbio.2004.02.002

Synthesis and evaluation of a fluorine-18 labeled antisense oligonucleotide as a potential PET tracer for iNOS mRNA expression

Erik F. J. de Vries
, Joke Vroegh
, Gerard Dijkstra
, Han Moshage
, Philip H. Elsinga
, Peter L. M. Jansen
, Willem Vaalburg

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Inducible NO synthase (iNOS) is overexpressed in inflammatory bowel diseases. An antisense oligonucleotide with good hybridization properties for iNOS mRNA was selected using RT-PCR. The oligonucleotide was reliably labeled with fluorine-18 using N-(4-[(18)F]fluorobenzyl)-2-bromoacetamide. Cellular uptake and efflux of oligonucleotide complexed with FuGENE-6 were rapid, unlike naked oligonucleotide, which hardly accumulated. However, neither uptake nor efflux showed any selectivity for iNOS expressing cells. The oligonucleotide showed a high level of non-specific binding, which may have obscured its specific hybridization to iNOS mRNA

Original language	English
Pages (from-to)	605-612
Journal	Nuclear medicine and biology
Volume	31
Issue number	5
DOIs	https://doi.org/10.1016/j.nucmedbio.2004.02.002
Publication status	Published - 2004

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10.1016/j.nucmedbio.2004.02.002

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title = "Synthesis and evaluation of a fluorine-18 labeled antisense oligonucleotide as a potential PET tracer for iNOS mRNA expression",

abstract = "Inducible NO synthase (iNOS) is overexpressed in inflammatory bowel diseases. An antisense oligonucleotide with good hybridization properties for iNOS mRNA was selected using RT-PCR. The oligonucleotide was reliably labeled with fluorine-18 using N-(4-[(18)F]fluorobenzyl)-2-bromoacetamide. Cellular uptake and efflux of oligonucleotide complexed with FuGENE-6 were rapid, unlike naked oligonucleotide, which hardly accumulated. However, neither uptake nor efflux showed any selectivity for iNOS expressing cells. The oligonucleotide showed a high level of non-specific binding, which may have obscured its specific hybridization to iNOS mRNA",

author = "\{de Vries\}, \{Erik F. J.\} and Joke Vroegh and Gerard Dijkstra and Han Moshage and Elsinga, \{Philip H.\} and Jansen, \{Peter L. M.\} and Willem Vaalburg",

year = "2004",

doi = "10.1016/j.nucmedbio.2004.02.002",

language = "English",

volume = "31",

pages = "605--612",

journal = "Nuclear medicine and biology",

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TY - JOUR

T1 - Synthesis and evaluation of a fluorine-18 labeled antisense oligonucleotide as a potential PET tracer for iNOS mRNA expression

AU - de Vries, Erik F. J.

AU - Vroegh, Joke

AU - Dijkstra, Gerard

AU - Moshage, Han

AU - Elsinga, Philip H.

AU - Jansen, Peter L. M.

AU - Vaalburg, Willem

PY - 2004

Y1 - 2004

N2 - Inducible NO synthase (iNOS) is overexpressed in inflammatory bowel diseases. An antisense oligonucleotide with good hybridization properties for iNOS mRNA was selected using RT-PCR. The oligonucleotide was reliably labeled with fluorine-18 using N-(4-[(18)F]fluorobenzyl)-2-bromoacetamide. Cellular uptake and efflux of oligonucleotide complexed with FuGENE-6 were rapid, unlike naked oligonucleotide, which hardly accumulated. However, neither uptake nor efflux showed any selectivity for iNOS expressing cells. The oligonucleotide showed a high level of non-specific binding, which may have obscured its specific hybridization to iNOS mRNA

AB - Inducible NO synthase (iNOS) is overexpressed in inflammatory bowel diseases. An antisense oligonucleotide with good hybridization properties for iNOS mRNA was selected using RT-PCR. The oligonucleotide was reliably labeled with fluorine-18 using N-(4-[(18)F]fluorobenzyl)-2-bromoacetamide. Cellular uptake and efflux of oligonucleotide complexed with FuGENE-6 were rapid, unlike naked oligonucleotide, which hardly accumulated. However, neither uptake nor efflux showed any selectivity for iNOS expressing cells. The oligonucleotide showed a high level of non-specific binding, which may have obscured its specific hybridization to iNOS mRNA

U2 - 10.1016/j.nucmedbio.2004.02.002

DO - 10.1016/j.nucmedbio.2004.02.002

M3 - Article

C2 - 15219279

SN - 0969-8051

VL - 31

SP - 605

EP - 612

JO - Nuclear medicine and biology

JF - Nuclear medicine and biology

IS - 5

ER -

Synthesis and evaluation of a fluorine-18 labeled antisense oligonucleotide as a potential PET tracer for iNOS mRNA expression

Abstract

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