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Studying nanoparticle-3D tumour spheroid interactions in a microfluidic format: Towards the assessment of drug delivery to complex tumour tissues under physiological conditions

  • Jean Baptiste Blondé*
  • , Dhanya Babu
  • , Dwi Priwitaningrum
  • , Adithya Sridhar
  • , Jai Prakash
  • , Séverine Le Gac
  • *Corresponding author for this work
  • University of Twente

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

Abstract

Here, we report a microfluidic platform to investigate interactions between drug-loaded nanoconstructs and 3D tumour spheroids under static conditions and flow-based exposure. Experiments are performed using fluorescently labelled silica nanoparticles of various sizes and with different surface coatings, and by measuring optically the nanoparticle penetration depth in tumour spheroids trapped in our microfluidic device. Confocal microscopy imaging reveals superficial penetration of nanoparticles in the tumour spheroids, inversely proportional to the size of the nanoconstructs, and increased penetration under dynamic conditions created by a laminar flow.

Original languageEnglish
Title of host publication20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PublisherChemical and Biological Microsystems Society
Pages529-530
Number of pages2
ISBN (Electronic)9780979806490
Publication statusPublished - 2016
Event20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland
Duration: 9 Oct 201613 Oct 2016

Publication series

Name20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Conference

Conference20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Country/TerritoryIreland
CityDublin
Period09/10/201613/10/2016

Keywords

  • Drug delivery and penetration
  • Microfluidics
  • Nanoparticles
  • Tumour spheroids

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