Sensitivity and specificity of the Leishmania OligoC-TesT and NASBA-oligochromatography for diagnosis of visceral leishmaniasis in Kenya

Frank L. Basiye; Margaret Mbuchi; Charles Magiri; George Kirigi; Stijn Deborggraeve; Gerard J. Schoone; Alfarazdeg A. Saad; Sayda el-Safi; Enock Matovu; Monique K. Wasunna

doi:10.1111/j.1365-3156.2010.02548.x

Sensitivity and specificity of the Leishmania OligoC-TesT and NASBA-oligochromatography for diagnosis of visceral leishmaniasis in Kenya

Frank L. Basiye
, Margaret Mbuchi
, Charles Magiri
, George Kirigi
, Stijn Deborggraeve
, Gerard J. Schoone
, Alfarazdeg A. Saad
, Sayda el-Safi
, Enock Matovu
, Monique K. Wasunna

Kenya Medical Research Institute
Institute of Tropical Medicine Antwerp
KU Leuven
Royal Tropical Institute
University of Khartoum
Makerere University

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Objective: To estimate the sensitivity and specificity of the OligoC-TesT and nucleic acid sequence-based amplification coupled to oligochromatography (NASBA-OC) for molecular detection of Leishmania in blood from patients with confirmed visceral leishmaniasis (VL) and healthy endemic controls from Kenya. Methods: Blood specimens of 84 patients with confirmed VL and 98 endemic healthy controls from Baringo district in Kenya were submitted to both assays. Results: The Leishmania OligoC-TesT showed a sensitivity of 96.4% (95% confidence interval [CI]: 90-98.8%) and a specificity of 88.8% (95% CI: 81-93.6%), while the sensitivity and specificity of the NASBA-OC were 79.8% (95% CI: 67-87%) and 100% (95% CI: 96.3-100%), respectively. Conclusion: Our findings indicate high sensitivity of the Leishmania OligoC-TesT on blood while the NASBA-OC is a better marker for active disease. © 2010 Blackwell Publishing Ltd.

Original language	English
Pages (from-to)	806-810
Journal	Tropical Medicine and International Health
Volume	15
Issue number	7
DOIs	https://doi.org/10.1111/j.1365-3156.2010.02548.x
Publication status	Published - Jul 2010
Externally published	Yes

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Basiye, F. L., Mbuchi, M., Magiri, C., Kirigi, G., Deborggraeve, S., Schoone, G. J., Saad, A. A., el-Safi, S., Matovu, E., & Wasunna, M. K. (2010). Sensitivity and specificity of the Leishmania OligoC-TesT and NASBA-oligochromatography for diagnosis of visceral leishmaniasis in Kenya. Tropical Medicine and International Health, 15(7), 806-810. https://doi.org/10.1111/j.1365-3156.2010.02548.x

@article{c92da76af20d4b7f9bd94399085cd916,

title = "Sensitivity and specificity of the Leishmania OligoC-TesT and NASBA-oligochromatography for diagnosis of visceral leishmaniasis in Kenya",

abstract = "Objective: To estimate the sensitivity and specificity of the OligoC-TesT and nucleic acid sequence-based amplification coupled to oligochromatography (NASBA-OC) for molecular detection of Leishmania in blood from patients with confirmed visceral leishmaniasis (VL) and healthy endemic controls from Kenya. Methods: Blood specimens of 84 patients with confirmed VL and 98 endemic healthy controls from Baringo district in Kenya were submitted to both assays. Results: The Leishmania OligoC-TesT showed a sensitivity of 96.4\% (95\% confidence interval [CI]: 90-98.8\%) and a specificity of 88.8\% (95\% CI: 81-93.6\%), while the sensitivity and specificity of the NASBA-OC were 79.8\% (95\% CI: 67-87\%) and 100\% (95\% CI: 96.3-100\%), respectively. Conclusion: Our findings indicate high sensitivity of the Leishmania OligoC-TesT on blood while the NASBA-OC is a better marker for active disease. {\textcopyright} 2010 Blackwell Publishing Ltd.",

author = "Basiye, \{Frank L.\} and Margaret Mbuchi and Charles Magiri and George Kirigi and Stijn Deborggraeve and Schoone, \{Gerard J.\} and Saad, \{Alfarazdeg A.\} and Sayda el-Safi and Enock Matovu and Wasunna, \{Monique K.\}",

year = "2010",

month = jul,

doi = "10.1111/j.1365-3156.2010.02548.x",

language = "English",

volume = "15",

pages = "806--810",

journal = "Tropical Medicine and International Health",

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Basiye, FL, Mbuchi, M, Magiri, C, Kirigi, G, Deborggraeve, S, Schoone, GJ, Saad, AA, el-Safi, S, Matovu, E & Wasunna, MK 2010, 'Sensitivity and specificity of the Leishmania OligoC-TesT and NASBA-oligochromatography for diagnosis of visceral leishmaniasis in Kenya', Tropical Medicine and International Health, vol. 15, no. 7, pp. 806-810. https://doi.org/10.1111/j.1365-3156.2010.02548.x

TY - JOUR

T1 - Sensitivity and specificity of the Leishmania OligoC-TesT and NASBA-oligochromatography for diagnosis of visceral leishmaniasis in Kenya

AU - Basiye, Frank L.

AU - Mbuchi, Margaret

AU - Magiri, Charles

AU - Kirigi, George

AU - Deborggraeve, Stijn

AU - Schoone, Gerard J.

AU - Saad, Alfarazdeg A.

AU - el-Safi, Sayda

AU - Matovu, Enock

AU - Wasunna, Monique K.

PY - 2010/7

Y1 - 2010/7

N2 - Objective: To estimate the sensitivity and specificity of the OligoC-TesT and nucleic acid sequence-based amplification coupled to oligochromatography (NASBA-OC) for molecular detection of Leishmania in blood from patients with confirmed visceral leishmaniasis (VL) and healthy endemic controls from Kenya. Methods: Blood specimens of 84 patients with confirmed VL and 98 endemic healthy controls from Baringo district in Kenya were submitted to both assays. Results: The Leishmania OligoC-TesT showed a sensitivity of 96.4% (95% confidence interval [CI]: 90-98.8%) and a specificity of 88.8% (95% CI: 81-93.6%), while the sensitivity and specificity of the NASBA-OC were 79.8% (95% CI: 67-87%) and 100% (95% CI: 96.3-100%), respectively. Conclusion: Our findings indicate high sensitivity of the Leishmania OligoC-TesT on blood while the NASBA-OC is a better marker for active disease. © 2010 Blackwell Publishing Ltd.

AB - Objective: To estimate the sensitivity and specificity of the OligoC-TesT and nucleic acid sequence-based amplification coupled to oligochromatography (NASBA-OC) for molecular detection of Leishmania in blood from patients with confirmed visceral leishmaniasis (VL) and healthy endemic controls from Kenya. Methods: Blood specimens of 84 patients with confirmed VL and 98 endemic healthy controls from Baringo district in Kenya were submitted to both assays. Results: The Leishmania OligoC-TesT showed a sensitivity of 96.4% (95% confidence interval [CI]: 90-98.8%) and a specificity of 88.8% (95% CI: 81-93.6%), while the sensitivity and specificity of the NASBA-OC were 79.8% (95% CI: 67-87%) and 100% (95% CI: 96.3-100%), respectively. Conclusion: Our findings indicate high sensitivity of the Leishmania OligoC-TesT on blood while the NASBA-OC is a better marker for active disease. © 2010 Blackwell Publishing Ltd.

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UR - https://www.ncbi.nlm.nih.gov/pubmed/20487428

U2 - 10.1111/j.1365-3156.2010.02548.x

DO - 10.1111/j.1365-3156.2010.02548.x

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SN - 1360-2276

VL - 15

SP - 806

EP - 810

JO - Tropical Medicine and International Health

JF - Tropical Medicine and International Health

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ER -

Sensitivity and specificity of the Leishmania OligoC-TesT and NASBA-oligochromatography for diagnosis of visceral leishmaniasis in Kenya

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