TY - JOUR
T1 - Sarcosin (Krp1) in skeletal muscle differentiation: gene expression profiling and knockdown experiments
AU - Du Puy, Leonie
AU - Beqqali, Abdelaziz
AU - van Tol, Helena T. A.
AU - Monshouwer-Kloots, Jantine
AU - Passier, Robert
AU - Haagsman, Henk P.
AU - Roelen, Bernard A. J.
PY - 2012
Y1 - 2012
N2 - SARCOSIN, also named Krp1, has been identified as a protein exclusively expressed in striated muscle tissue. Here we report on the role of SARCOSIN in skeletal muscle development and differentiation. We demonstrate, by means of whole-mount in situ hybridization, that Sarcosin mRNA is expressed in the myotome part of the mature somites in mouse embryos from embryonic day 9.5 onwards. Sarcosin is not expressed in the developing heart at these embryonic stages, and in adult tissues the mRNA expression levels are five times lower in the heart than in skeletal muscle. SARCOSIN protein partially co-localizes with the M-band protein myomesin and between and below laterally fusing myofibrils in adult skeletal muscle tissue. RNA interference mediated knock-down of SARCOSIN in the C2C12 myoblast cell line appeared to be stimulatory in the early phase of differentiation, but inhibitory at a later phase of differentiation
AB - SARCOSIN, also named Krp1, has been identified as a protein exclusively expressed in striated muscle tissue. Here we report on the role of SARCOSIN in skeletal muscle development and differentiation. We demonstrate, by means of whole-mount in situ hybridization, that Sarcosin mRNA is expressed in the myotome part of the mature somites in mouse embryos from embryonic day 9.5 onwards. Sarcosin is not expressed in the developing heart at these embryonic stages, and in adult tissues the mRNA expression levels are five times lower in the heart than in skeletal muscle. SARCOSIN protein partially co-localizes with the M-band protein myomesin and between and below laterally fusing myofibrils in adult skeletal muscle tissue. RNA interference mediated knock-down of SARCOSIN in the C2C12 myoblast cell line appeared to be stimulatory in the early phase of differentiation, but inhibitory at a later phase of differentiation
U2 - 10.1387/ijdb.113327lp
DO - 10.1387/ijdb.113327lp
M3 - Article
C2 - 22562206
SN - 0214-6282
VL - 56
SP - 301
EP - 309
JO - International journal of developmental biology
JF - International journal of developmental biology
IS - 4
ER -