Regional and temporal expression patterns of interleukin-10, interleukin-10 receptor and adhesion molecules in the rat spinal cord during chronic relapsing EAE

Annemarie Ledeboer; Anne Wierinckx; John G. J. M. Bol; Sarah Floris; Chantal Renardel de Lavalette; Helga E. de Vries; Timo K. van den Berg; Christine D. Dijkstra; Fred J. H. Tilders; Anne-Marie van Dam

doi:10.1016/S0165-5728(03)00031-6

Regional and temporal expression patterns of interleukin-10, interleukin-10 receptor and adhesion molecules in the rat spinal cord during chronic relapsing EAE

Annemarie Ledeboer, Anne Wierinckx, John G. J. M. Bol, Sarah Floris, Chantal Renardel de Lavalette, Helga E. de Vries, Timo K. van den Berg, Christine D. Dijkstra, Fred J. H. Tilders, Anne-Marie van Dam

Department of Medical Pharmacology, Research Institute Neurosciences, VU University Medical Center, Van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands.

Research output: Contribution to journal › Article › Academic › peer-review

48 Citations (Scopus)

Abstract

Adhesion molecules intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) mediate leukocyte infiltration into the CNS, in experimental autoimmune encephalomyelitis (EAE) and multiple sclerosis (MS). Because exogenous interleukin-10 (IL-10) inhibits ICAM-1 and VCAM-1 expression and clinical EAE, we hypothesize that endogenous IL-10 signaling may suppress expression of adhesion molecules. In a rat model of chronic relapsing EAE, expression levels of IL-10 and its receptor (IL-10R1), ICAM-1 and VCAM-1 mRNA in the spinal cord are markedly increased, whereas levels of IL-10 mRNA remain relatively low. The temporal pattern of mRNA and protein expression showed marked differences between spinal cord levels. During relapse, IL-10, IL-10R1, ICAM-1, VCAM-1 mRNA levels and neurological scores show positive correlations. We conclude that endogenous IL-10 is not a crucial factor inhibiting adhesion molecule expression in this model

Original language	English
Pages (from-to)	94-103
Number of pages	10
Journal	Journal of neuroimmunology
Volume	136
Issue number	1-2
DOIs	https://doi.org/10.1016/S0165-5728(03)00031-6
Publication status	Published - Mar 2003

Keywords

Animals
Cell Adhesion Molecules
Chemotaxis, Leukocyte
Chronic Disease
Disease Models, Animal
Encephalomyelitis, Autoimmune, Experimental
Gene Expression Regulation
Immunohistochemistry
Intercellular Adhesion Molecule-1
Interleukin-10
Male
Multiple Sclerosis, Relapsing-Remitting
RNA, Messenger
Rats
Rats, Inbred Strains
Reaction Time
Receptors, Interleukin
Receptors, Interleukin-10
Spinal Cord
Time Factors
Vascular Cell Adhesion Molecule-1
Journal Article
Research Support, Non-U.S. Gov't

Access to Document

10.1016/S0165-5728(03)00031-6

Cite this

Ledeboer, A., Wierinckx, A., Bol, J. G. J. M., Floris, S., Renardel de Lavalette, C., de Vries, H. E., van den Berg, T. K., Dijkstra, C. D., Tilders, F. J. H., & van Dam, A.-M. (2003). Regional and temporal expression patterns of interleukin-10, interleukin-10 receptor and adhesion molecules in the rat spinal cord during chronic relapsing EAE. Journal of neuroimmunology, 136(1-2), 94-103. https://doi.org/10.1016/S0165-5728(03)00031-6

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title = "Regional and temporal expression patterns of interleukin-10, interleukin-10 receptor and adhesion molecules in the rat spinal cord during chronic relapsing EAE",

abstract = "Adhesion molecules intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) mediate leukocyte infiltration into the CNS, in experimental autoimmune encephalomyelitis (EAE) and multiple sclerosis (MS). Because exogenous interleukin-10 (IL-10) inhibits ICAM-1 and VCAM-1 expression and clinical EAE, we hypothesize that endogenous IL-10 signaling may suppress expression of adhesion molecules. In a rat model of chronic relapsing EAE, expression levels of IL-10 and its receptor (IL-10R1), ICAM-1 and VCAM-1 mRNA in the spinal cord are markedly increased, whereas levels of IL-10 mRNA remain relatively low. The temporal pattern of mRNA and protein expression showed marked differences between spinal cord levels. During relapse, IL-10, IL-10R1, ICAM-1, VCAM-1 mRNA levels and neurological scores show positive correlations. We conclude that endogenous IL-10 is not a crucial factor inhibiting adhesion molecule expression in this model",

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author = "Annemarie Ledeboer and Anne Wierinckx and Bol, {John G. J. M.} and Sarah Floris and {Renardel de Lavalette}, Chantal and {de Vries}, {Helga E.} and {van den Berg}, {Timo K.} and Dijkstra, {Christine D.} and Tilders, {Fred J. H.} and {van Dam}, Anne-Marie",

year = "2003",

month = mar,

doi = "10.1016/S0165-5728(03)00031-6",

language = "English",

volume = "136",

pages = "94--103",

journal = "Journal of neuroimmunology",

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Ledeboer, A, Wierinckx, A, Bol, JGJM, Floris, S, Renardel de Lavalette, C, de Vries, HE , van den Berg, TK, Dijkstra, CD, Tilders, FJH & van Dam, A-M 2003, 'Regional and temporal expression patterns of interleukin-10, interleukin-10 receptor and adhesion molecules in the rat spinal cord during chronic relapsing EAE', Journal of neuroimmunology, vol. 136, no. 1-2, pp. 94-103. https://doi.org/10.1016/S0165-5728(03)00031-6

Regional and temporal expression patterns of interleukin-10, interleukin-10 receptor and adhesion molecules in the rat spinal cord during chronic relapsing EAE. / Ledeboer, Annemarie; Wierinckx, Anne; Bol, John G. J. M. et al.
In: Journal of neuroimmunology, Vol. 136, No. 1-2, 03.2003, p. 94-103.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Regional and temporal expression patterns of interleukin-10, interleukin-10 receptor and adhesion molecules in the rat spinal cord during chronic relapsing EAE

AU - Ledeboer, Annemarie

AU - Wierinckx, Anne

AU - Bol, John G. J. M.

AU - Floris, Sarah

AU - Renardel de Lavalette, Chantal

AU - de Vries, Helga E.

AU - van den Berg, Timo K.

AU - Dijkstra, Christine D.

AU - Tilders, Fred J. H.

AU - van Dam, Anne-Marie

PY - 2003/3

Y1 - 2003/3

N2 - Adhesion molecules intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) mediate leukocyte infiltration into the CNS, in experimental autoimmune encephalomyelitis (EAE) and multiple sclerosis (MS). Because exogenous interleukin-10 (IL-10) inhibits ICAM-1 and VCAM-1 expression and clinical EAE, we hypothesize that endogenous IL-10 signaling may suppress expression of adhesion molecules. In a rat model of chronic relapsing EAE, expression levels of IL-10 and its receptor (IL-10R1), ICAM-1 and VCAM-1 mRNA in the spinal cord are markedly increased, whereas levels of IL-10 mRNA remain relatively low. The temporal pattern of mRNA and protein expression showed marked differences between spinal cord levels. During relapse, IL-10, IL-10R1, ICAM-1, VCAM-1 mRNA levels and neurological scores show positive correlations. We conclude that endogenous IL-10 is not a crucial factor inhibiting adhesion molecule expression in this model

AB - Adhesion molecules intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) mediate leukocyte infiltration into the CNS, in experimental autoimmune encephalomyelitis (EAE) and multiple sclerosis (MS). Because exogenous interleukin-10 (IL-10) inhibits ICAM-1 and VCAM-1 expression and clinical EAE, we hypothesize that endogenous IL-10 signaling may suppress expression of adhesion molecules. In a rat model of chronic relapsing EAE, expression levels of IL-10 and its receptor (IL-10R1), ICAM-1 and VCAM-1 mRNA in the spinal cord are markedly increased, whereas levels of IL-10 mRNA remain relatively low. The temporal pattern of mRNA and protein expression showed marked differences between spinal cord levels. During relapse, IL-10, IL-10R1, ICAM-1, VCAM-1 mRNA levels and neurological scores show positive correlations. We conclude that endogenous IL-10 is not a crucial factor inhibiting adhesion molecule expression in this model

KW - Animals

KW - Cell Adhesion Molecules

KW - Chemotaxis, Leukocyte

KW - Chronic Disease

KW - Disease Models, Animal

KW - Encephalomyelitis, Autoimmune, Experimental

KW - Gene Expression Regulation

KW - Immunohistochemistry

KW - Intercellular Adhesion Molecule-1

KW - Interleukin-10

KW - Male

KW - Multiple Sclerosis, Relapsing-Remitting

KW - RNA, Messenger

KW - Rats

KW - Rats, Inbred Strains

KW - Reaction Time

KW - Receptors, Interleukin

KW - Receptors, Interleukin-10

KW - Spinal Cord

KW - Time Factors

KW - Vascular Cell Adhesion Molecule-1

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1016/S0165-5728(03)00031-6

DO - 10.1016/S0165-5728(03)00031-6

M3 - Article

C2 - 12620647

SN - 0165-5728

VL - 136

SP - 94

EP - 103

JO - Journal of neuroimmunology

JF - Journal of neuroimmunology

IS - 1-2

ER -