Prognostic value of cell proliferation (Ki-67 antigen) and nuclear DNA content in clinically resectable, distal bile duct carcinoma

A. M. Rijken; A. Umezawa; T. M. van Gulik; A. Bosma; M. M. Polak; G. J. Offerhaus; H. Obertop; D. J. Gouma

doi:10.1007/BF02303480

Prognostic value of cell proliferation (Ki-67 antigen) and nuclear DNA content in clinically resectable, distal bile duct carcinoma

A. M. Rijken
, A. Umezawa
, T. M. van Gulik
, A. Bosma
, M. M. Polak
, G. J. Offerhaus
, H. Obertop
, D. J. Gouma

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

BACKGROUND: The aim of this study was to investigate the prognostic value of cell proliferation (Ki-67 antigen) and DNA content in patients resected for distal bile duct carcinoma (DBDC). METHODS: Formalin-fixed tumor specimens of 35 patients with resected DBDC and a long-term clinical follow-up were analyzed. MIB-1 antibody was used for Ki-67 antigen detection to determine the proportion of proliferating cells. DNA content was measured using flow cytometry. RESULTS: A significant correlation was found between a low MIB-1 index ( <20%) and survival (P <.05). Of the 35 tumor specimens, 34 specimens were evaluable by flow cytometry: 22 carcinomas were diploid (65%), and 12 were aneuploid (35%). The median DNA index of aneuploid tumors was 1.36 (range, 1.09 to 1.76). No correlation of DNA-ploidy with survival time was found. CONCLUSION: In contrast to DNA-ploidy pattern, Ki-67 antigen expression showed prognostic significance in resectable DBDC. A Ki-67 positive ratio of > or =20% was associated with decreased survival time

Original language	English
Pages (from-to)	699-705
Journal	Annals of surgical oncology
Volume	5
Issue number	8
DOIs	https://doi.org/10.1007/BF02303480
Publication status	Published - 1998

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Access to Document

10.1007/BF02303480

Cite this

@article{e009306298534c8fa0fab52ae9a70d8f,

title = "Prognostic value of cell proliferation (Ki-67 antigen) and nuclear DNA content in clinically resectable, distal bile duct carcinoma",

abstract = "BACKGROUND: The aim of this study was to investigate the prognostic value of cell proliferation (Ki-67 antigen) and DNA content in patients resected for distal bile duct carcinoma (DBDC). METHODS: Formalin-fixed tumor specimens of 35 patients with resected DBDC and a long-term clinical follow-up were analyzed. MIB-1 antibody was used for Ki-67 antigen detection to determine the proportion of proliferating cells. DNA content was measured using flow cytometry. RESULTS: A significant correlation was found between a low MIB-1 index ( <20\%) and survival (P <.05). Of the 35 tumor specimens, 34 specimens were evaluable by flow cytometry: 22 carcinomas were diploid (65\%), and 12 were aneuploid (35\%). The median DNA index of aneuploid tumors was 1.36 (range, 1.09 to 1.76). No correlation of DNA-ploidy with survival time was found. CONCLUSION: In contrast to DNA-ploidy pattern, Ki-67 antigen expression showed prognostic significance in resectable DBDC. A Ki-67 positive ratio of > or =20\% was associated with decreased survival time",

author = "Rijken, \{A. M.\} and A. Umezawa and \{van Gulik\}, \{T. M.\} and A. Bosma and Polak, \{M. M.\} and Offerhaus, \{G. J.\} and H. Obertop and Gouma, \{D. J.\}",

year = "1998",

doi = "10.1007/BF02303480",

language = "English",

volume = "5",

pages = "699--705",

journal = "Annals of surgical oncology",

issn = "1068-9265",

publisher = "Springer New York",

number = "8",

}

TY - JOUR

T1 - Prognostic value of cell proliferation (Ki-67 antigen) and nuclear DNA content in clinically resectable, distal bile duct carcinoma

AU - Rijken, A. M.

AU - Umezawa, A.

AU - van Gulik, T. M.

AU - Bosma, A.

AU - Polak, M. M.

AU - Offerhaus, G. J.

AU - Obertop, H.

AU - Gouma, D. J.

PY - 1998

Y1 - 1998

N2 - BACKGROUND: The aim of this study was to investigate the prognostic value of cell proliferation (Ki-67 antigen) and DNA content in patients resected for distal bile duct carcinoma (DBDC). METHODS: Formalin-fixed tumor specimens of 35 patients with resected DBDC and a long-term clinical follow-up were analyzed. MIB-1 antibody was used for Ki-67 antigen detection to determine the proportion of proliferating cells. DNA content was measured using flow cytometry. RESULTS: A significant correlation was found between a low MIB-1 index ( <20%) and survival (P <.05). Of the 35 tumor specimens, 34 specimens were evaluable by flow cytometry: 22 carcinomas were diploid (65%), and 12 were aneuploid (35%). The median DNA index of aneuploid tumors was 1.36 (range, 1.09 to 1.76). No correlation of DNA-ploidy with survival time was found. CONCLUSION: In contrast to DNA-ploidy pattern, Ki-67 antigen expression showed prognostic significance in resectable DBDC. A Ki-67 positive ratio of > or =20% was associated with decreased survival time

AB - BACKGROUND: The aim of this study was to investigate the prognostic value of cell proliferation (Ki-67 antigen) and DNA content in patients resected for distal bile duct carcinoma (DBDC). METHODS: Formalin-fixed tumor specimens of 35 patients with resected DBDC and a long-term clinical follow-up were analyzed. MIB-1 antibody was used for Ki-67 antigen detection to determine the proportion of proliferating cells. DNA content was measured using flow cytometry. RESULTS: A significant correlation was found between a low MIB-1 index ( <20%) and survival (P <.05). Of the 35 tumor specimens, 34 specimens were evaluable by flow cytometry: 22 carcinomas were diploid (65%), and 12 were aneuploid (35%). The median DNA index of aneuploid tumors was 1.36 (range, 1.09 to 1.76). No correlation of DNA-ploidy with survival time was found. CONCLUSION: In contrast to DNA-ploidy pattern, Ki-67 antigen expression showed prognostic significance in resectable DBDC. A Ki-67 positive ratio of > or =20% was associated with decreased survival time

U2 - 10.1007/BF02303480

DO - 10.1007/BF02303480

M3 - Article

C2 - 9869516

SN - 1068-9265

VL - 5

SP - 699

EP - 705

JO - Annals of surgical oncology

JF - Annals of surgical oncology

IS - 8

ER -

Prognostic value of cell proliferation (Ki-67 antigen) and nuclear DNA content in clinically resectable, distal bile duct carcinoma

Abstract

UN SDGs

Access to Document

Fingerprint

Cite this