Preparation of chromosome suspensions from cells of a solid experimental tumour for measurement by flow cytometry

M. W. Kooi; J. A. Aten; J. Stap; J. B. Kipp; G. W. Barendsen

doi:10.1002/cyto.990050519

Preparation of chromosome suspensions from cells of a solid experimental tumour for measurement by flow cytometry

M. W. Kooi
, J. A. Aten
, J. Stap
, J. B. Kipp
, G. W. Barendsen

Research output: Contribution to journal › Comment/Letter to the editor › Academic

Abstract

A method is described by which metaphase chromosomes are isolated from cells of a transplantable rat sarcoma. The chromosomes are derived from cells in a suspension prepared by trypsinisation of tumours from rats that have been treated with vindesine 24 h before excision in order to accumulate cells in mitosis. Histograms obtained for the chromosomes of the solid tumour are compared with flow karyotypes of cells cultured for 20 h or for several generations in vitro

Original language	English
Pages (from-to)	547-549
Journal	Cytometry
Volume	5
Issue number	5
DOIs	https://doi.org/10.1002/cyto.990050519
Publication status	Published - 1984

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10.1002/cyto.990050519

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title = "Preparation of chromosome suspensions from cells of a solid experimental tumour for measurement by flow cytometry",

abstract = "A method is described by which metaphase chromosomes are isolated from cells of a transplantable rat sarcoma. The chromosomes are derived from cells in a suspension prepared by trypsinisation of tumours from rats that have been treated with vindesine 24 h before excision in order to accumulate cells in mitosis. Histograms obtained for the chromosomes of the solid tumour are compared with flow karyotypes of cells cultured for 20 h or for several generations in vitro",

author = "Kooi, \{M. W.\} and Aten, \{J. A.\} and J. Stap and Kipp, \{J. B.\} and Barendsen, \{G. W.\}",

year = "1984",

doi = "10.1002/cyto.990050519",

language = "English",

volume = "5",

pages = "547--549",

journal = "Cytometry",

issn = "0196-4763",

publisher = "John Wiley and Sons Inc.",

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TY - JOUR

T1 - Preparation of chromosome suspensions from cells of a solid experimental tumour for measurement by flow cytometry

AU - Kooi, M. W.

AU - Aten, J. A.

AU - Stap, J.

AU - Kipp, J. B.

AU - Barendsen, G. W.

PY - 1984

Y1 - 1984

N2 - A method is described by which metaphase chromosomes are isolated from cells of a transplantable rat sarcoma. The chromosomes are derived from cells in a suspension prepared by trypsinisation of tumours from rats that have been treated with vindesine 24 h before excision in order to accumulate cells in mitosis. Histograms obtained for the chromosomes of the solid tumour are compared with flow karyotypes of cells cultured for 20 h or for several generations in vitro

AB - A method is described by which metaphase chromosomes are isolated from cells of a transplantable rat sarcoma. The chromosomes are derived from cells in a suspension prepared by trypsinisation of tumours from rats that have been treated with vindesine 24 h before excision in order to accumulate cells in mitosis. Histograms obtained for the chromosomes of the solid tumour are compared with flow karyotypes of cells cultured for 20 h or for several generations in vitro

U2 - 10.1002/cyto.990050519

DO - 10.1002/cyto.990050519

M3 - Comment/Letter to the editor

C2 - 6489067

SN - 0196-4763

VL - 5

SP - 547

EP - 549

JO - Cytometry

JF - Cytometry

IS - 5

ER -

Preparation of chromosome suspensions from cells of a solid experimental tumour for measurement by flow cytometry

Abstract

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