Phytanic acid alpha-oxidation: identification of 2-hydroxyphytanoyl-CoA lyase in rat liver and its localisation in peroxisomes

G. A. Jansen; N. M. Verhoeven; S. Denis; G. Romeijn; C. Jakobs; H. J. ten Brink; R. J. Wanders

doi:10.1016/S1388-1981(99)00126-2

Phytanic acid alpha-oxidation: identification of 2-hydroxyphytanoyl-CoA lyase in rat liver and its localisation in peroxisomes

G. A. Jansen, N. M. Verhoeven, S. Denis, G. Romeijn, C. Jakobs, H. J. ten Brink, R. J. Wanders

Research output: Contribution to journal › Article › Academic › peer-review

26 Citations (Scopus)

Abstract

Phytanic acid is broken down by alpha-oxidation in three steps finally yielding pristanic acid. The first step occurs in peroxisomes and is catalysed by phytanoyl-CoA hydroxylase. We have studied the second step in the alpha-oxidation pathway, which involves conversion of 2-hydroxyphytanoyl-CoA to pristanal catalysed by 2-hydroxyphytanoyl-CoA lyase. To this end, we have developed a stable isotope dilution gas chromatography-mass spectrometry assay allowing activity measurements in rat liver homogenates. Cell fractionation studies demonstrate that in rat liver 2-hydroxyphytanoyl-CoA lyase is localised in peroxisomes. This finding may have important implications for inherited diseases in man characterised by impaired phytanic acid alpha-oxidation

Original language	English
Pages (from-to)	176-182
Journal	BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
Volume	1440
Issue number	2-3
DOIs	https://doi.org/10.1016/S1388-1981(99)00126-2
Publication status	Published - 1999

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10.1016/S1388-1981(99)00126-2

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Jansen, G. A., Verhoeven, N. M., Denis, S., Romeijn, G., Jakobs, C., ten Brink, H. J., & Wanders, R. J. (1999). Phytanic acid alpha-oxidation: identification of 2-hydroxyphytanoyl-CoA lyase in rat liver and its localisation in peroxisomes. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, 1440(2-3), 176-182. https://doi.org/10.1016/S1388-1981(99)00126-2

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title = "Phytanic acid alpha-oxidation: identification of 2-hydroxyphytanoyl-CoA lyase in rat liver and its localisation in peroxisomes",

abstract = "Phytanic acid is broken down by alpha-oxidation in three steps finally yielding pristanic acid. The first step occurs in peroxisomes and is catalysed by phytanoyl-CoA hydroxylase. We have studied the second step in the alpha-oxidation pathway, which involves conversion of 2-hydroxyphytanoyl-CoA to pristanal catalysed by 2-hydroxyphytanoyl-CoA lyase. To this end, we have developed a stable isotope dilution gas chromatography-mass spectrometry assay allowing activity measurements in rat liver homogenates. Cell fractionation studies demonstrate that in rat liver 2-hydroxyphytanoyl-CoA lyase is localised in peroxisomes. This finding may have important implications for inherited diseases in man characterised by impaired phytanic acid alpha-oxidation",

author = "Jansen, {G. A.} and Verhoeven, {N. M.} and S. Denis and G. Romeijn and C. Jakobs and {ten Brink}, {H. J.} and Wanders, {R. J.}",

year = "1999",

doi = "10.1016/S1388-1981(99)00126-2",

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pages = "176--182",

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Jansen, GA, Verhoeven, NM, Denis, S, Romeijn, G, Jakobs, C, ten Brink, HJ & Wanders, RJ 1999, 'Phytanic acid alpha-oxidation: identification of 2-hydroxyphytanoyl-CoA lyase in rat liver and its localisation in peroxisomes', BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, vol. 1440, no. 2-3, pp. 176-182. https://doi.org/10.1016/S1388-1981(99)00126-2

Phytanic acid alpha-oxidation: identification of 2-hydroxyphytanoyl-CoA lyase in rat liver and its localisation in peroxisomes. / Jansen, G. A.; Verhoeven, N. M.; Denis, S. et al.
In: BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, Vol. 1440, No. 2-3, 1999, p. 176-182.

Research output: Contribution to journal › Article › Academic › peer-review

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T1 - Phytanic acid alpha-oxidation: identification of 2-hydroxyphytanoyl-CoA lyase in rat liver and its localisation in peroxisomes

AU - Jansen, G. A.

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AU - ten Brink, H. J.

AU - Wanders, R. J.

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AB - Phytanic acid is broken down by alpha-oxidation in three steps finally yielding pristanic acid. The first step occurs in peroxisomes and is catalysed by phytanoyl-CoA hydroxylase. We have studied the second step in the alpha-oxidation pathway, which involves conversion of 2-hydroxyphytanoyl-CoA to pristanal catalysed by 2-hydroxyphytanoyl-CoA lyase. To this end, we have developed a stable isotope dilution gas chromatography-mass spectrometry assay allowing activity measurements in rat liver homogenates. Cell fractionation studies demonstrate that in rat liver 2-hydroxyphytanoyl-CoA lyase is localised in peroxisomes. This finding may have important implications for inherited diseases in man characterised by impaired phytanic acid alpha-oxidation

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