Online monitoring of myocardial bioprosthesis for cardiac repair

Cristina Prat-Vidal; Carolina Gálvez-Montón; Verónica Puig-Sanvicens; Benjamin Sanchez; Idoia Díaz-Güemes; Paco Bogónez-Franco; Isaac Perea-Gil; Anna Casas-Solà; Santiago Roura; Aida Llucià-Valldeperas; Carolina Soler-Botija; Francisco M Sánchez-Margallo; Carlos E Semino; Ramon Bragos; Antoni Bayes-Genis

doi:10.1016/j.ijcard.2014.04.181

Online monitoring of myocardial bioprosthesis for cardiac repair

Cristina Prat-Vidal
, Carolina Gálvez-Montón
, Verónica Puig-Sanvicens
, Benjamin Sanchez
, Idoia Díaz-Güemes
, Paco Bogónez-Franco
, Isaac Perea-Gil
, Anna Casas-Solà
, Santiago Roura
, Aida Llucià-Valldeperas
, Carolina Soler-Botija
, Francisco M Sánchez-Margallo
, Carlos E Semino
, Ramon Bragos
, Antoni Bayes-Genis

ICREC (Heart Failure and Cardiac Regeneration) Research Lab, Health Sciences Research Institute Germans Trias i Pujol (IGTP), Cardiology Service, Hospital Universitari Germans Trias i Pujol, Badalona, Barcelona, Spain. Electronic address: [email protected].
ICREC (Heart Failure and Cardiac Regeneration) Research Lab, Health Sciences Research Institute Germans Trias i Pujol (IGTP), Cardiology Service, Hospital Universitari Germans Trias i Pujol, Badalona, Barcelona, Spain.
Department of Bioengineering, Institut Químic de Sarrià (IQS), Universitat Ramon Llull, Barcelona, Spain.
Electronic and Biomedical Instrumentation Group, Departament d'Enginyeria Electrònica, Universitat Politècnica de Catalunya (UPC), Barcelona, Spain.
Jesús Usón Minimally Invasive Surgery Centre, JUMISC, Cáceres, Spain.
ICREC (Heart Failure and Cardiac Regeneration) Research Lab, Health Sciences Research Institute Germans Trias i Pujol (IGTP), Cardiology Service, Hospital Universitari Germans Trias i Pujol, Badalona, Barcelona, Spain; Department of Medicine, University Autonomous of Barcelona (UAB), Barcelona, Spain. Electronic address: [email protected].

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

BACKGROUND/OBJECTIVES: The aim of this study was to develop a myocardial bioprosthesis for cardiac repair with an integrated online monitoring system. Myocardial infarction (MI) causes irreversible myocyte loss and scar formation. Tissue engineering to reduce myocardial scar size has been tested with variable success, yet scar formation and modulation by an engineered graft is incompletely characterized.

METHODS: Decellularized human pericardium was embedded using self-assembling peptide RAD16-I with or without GFP-labeled mediastinal adipose tissue-derived progenitor cells (MATPCs). Resulting bioprostheses were implanted over the ischemic myocardium in the swine model of MI (n=8 treated and n=5 control animals). For in vivo electrical impedance spectroscopy (EIS) monitoring, two electrodes were anchored to construct edges, covered by NanoGold particles and connected to an impedance-based implantable device. Histological evaluation was performed to identify and characterize GFP cells on post mortem myocardial sections.

RESULTS: Pluripotency, cardiomyogenic and endothelial potential and migratory capacity of porcine-derived MATPCs were demonstrated in vitro. Decellularization protocol efficiency, biodegradability, as well as in vitro biocompatibility after recellularization were also verified. One month after myocardial bioprosthesis implantation, morphometry revealed a 36% reduction in infarct area, Ki67(+)-GFP(+)-MATPCs were found at infarct core and border zones, and bioprosthesis vascularization was confirmed by presence of Griffonia simplicifolia lectin I (GSLI) B4 isolectin(+)-GFP(+)-MATPCs. Electrical impedance measurement at low and high frequencies (10 kHz-100 kHz) allowed online monitoring of scar maturation.

CONCLUSIONS: With clinical translation as ultimate goal, this myocardial bioprosthesis holds promise to be a viable candidate for human cardiac repair.

Original language	English
Pages (from-to)	654-61
Number of pages	8
Journal	International journal of cardiology
Volume	174
Issue number	3
DOIs	https://doi.org/10.1016/j.ijcard.2014.04.181
Publication status	Published - 1 Jul 2014

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Adipose Tissue/cytology
Aged
Animals
Bioprosthesis
Cardiac Surgical Procedures/methods
Cells, Cultured
Female
Humans
Male
Middle Aged
Monitoring, Ambulatory/methods
Myocardial Ischemia/pathology
Online Systems
Pericardium/cytology
Prosthesis Implantation/methods
Swine

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10.1016/j.ijcard.2014.04.181

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Prat-Vidal, C., Gálvez-Montón, C., Puig-Sanvicens, V., Sanchez, B., Díaz-Güemes, I., Bogónez-Franco, P., Perea-Gil, I., Casas-Solà, A., Roura, S., Llucià-Valldeperas, A., Soler-Botija, C., Sánchez-Margallo, F. M., Semino, C. E., Bragos, R., & Bayes-Genis, A. (2014). Online monitoring of myocardial bioprosthesis for cardiac repair. International journal of cardiology, 174(3), 654-61. https://doi.org/10.1016/j.ijcard.2014.04.181

@article{bfcc4707e3e043b59857c2be82f2f4b2,

title = "Online monitoring of myocardial bioprosthesis for cardiac repair",

abstract = "BACKGROUND/OBJECTIVES: The aim of this study was to develop a myocardial bioprosthesis for cardiac repair with an integrated online monitoring system. Myocardial infarction (MI) causes irreversible myocyte loss and scar formation. Tissue engineering to reduce myocardial scar size has been tested with variable success, yet scar formation and modulation by an engineered graft is incompletely characterized.METHODS: Decellularized human pericardium was embedded using self-assembling peptide RAD16-I with or without GFP-labeled mediastinal adipose tissue-derived progenitor cells (MATPCs). Resulting bioprostheses were implanted over the ischemic myocardium in the swine model of MI (n=8 treated and n=5 control animals). For in vivo electrical impedance spectroscopy (EIS) monitoring, two electrodes were anchored to construct edges, covered by NanoGold particles and connected to an impedance-based implantable device. Histological evaluation was performed to identify and characterize GFP cells on post mortem myocardial sections.RESULTS: Pluripotency, cardiomyogenic and endothelial potential and migratory capacity of porcine-derived MATPCs were demonstrated in vitro. Decellularization protocol efficiency, biodegradability, as well as in vitro biocompatibility after recellularization were also verified. One month after myocardial bioprosthesis implantation, morphometry revealed a 36\% reduction in infarct area, Ki67(+)-GFP(+)-MATPCs were found at infarct core and border zones, and bioprosthesis vascularization was confirmed by presence of Griffonia simplicifolia lectin I (GSLI) B4 isolectin(+)-GFP(+)-MATPCs. Electrical impedance measurement at low and high frequencies (10 kHz-100 kHz) allowed online monitoring of scar maturation.CONCLUSIONS: With clinical translation as ultimate goal, this myocardial bioprosthesis holds promise to be a viable candidate for human cardiac repair.",

keywords = "Adipose Tissue/cytology, Aged, Animals, Bioprosthesis, Cardiac Surgical Procedures/methods, Cells, Cultured, Female, Humans, Male, Middle Aged, Monitoring, Ambulatory/methods, Myocardial Ischemia/pathology, Online Systems, Pericardium/cytology, Prosthesis Implantation/methods, Swine",

author = "Cristina Prat-Vidal and Carolina G{\'a}lvez-Mont{\'o}n and Ver{\'o}nica Puig-Sanvicens and Benjamin Sanchez and Idoia D{\'i}az-G{\"u}emes and Paco Bog{\'o}nez-Franco and Isaac Perea-Gil and Anna Casas-Sol{\`a} and Santiago Roura and Aida Lluci{\`a}-Valldeperas and Carolina Soler-Botija and S{\'a}nchez-Margallo, \{Francisco M\} and Semino, \{Carlos E\} and Ramon Bragos and Antoni Bayes-Genis",

year = "2014",

month = jul,

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doi = "10.1016/j.ijcard.2014.04.181",

language = "English",

volume = "174",

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journal = "International journal of cardiology",

issn = "0167-5273",

publisher = "Elsevier Ireland Ltd",

number = "3",

}

Prat-Vidal, C, Gálvez-Montón, C, Puig-Sanvicens, V, Sanchez, B, Díaz-Güemes, I, Bogónez-Franco, P, Perea-Gil, I, Casas-Solà, A, Roura, S, Llucià-Valldeperas, A, Soler-Botija, C, Sánchez-Margallo, FM, Semino, CE, Bragos, R & Bayes-Genis, A 2014, 'Online monitoring of myocardial bioprosthesis for cardiac repair', International journal of cardiology, vol. 174, no. 3, pp. 654-61. https://doi.org/10.1016/j.ijcard.2014.04.181

TY - JOUR

T1 - Online monitoring of myocardial bioprosthesis for cardiac repair

AU - Prat-Vidal, Cristina

AU - Gálvez-Montón, Carolina

AU - Puig-Sanvicens, Verónica

AU - Sanchez, Benjamin

AU - Díaz-Güemes, Idoia

AU - Bogónez-Franco, Paco

AU - Perea-Gil, Isaac

AU - Casas-Solà, Anna

AU - Roura, Santiago

AU - Llucià-Valldeperas, Aida

AU - Soler-Botija, Carolina

AU - Sánchez-Margallo, Francisco M

AU - Semino, Carlos E

AU - Bragos, Ramon

AU - Bayes-Genis, Antoni

PY - 2014/7/1

Y1 - 2014/7/1

N2 - BACKGROUND/OBJECTIVES: The aim of this study was to develop a myocardial bioprosthesis for cardiac repair with an integrated online monitoring system. Myocardial infarction (MI) causes irreversible myocyte loss and scar formation. Tissue engineering to reduce myocardial scar size has been tested with variable success, yet scar formation and modulation by an engineered graft is incompletely characterized.METHODS: Decellularized human pericardium was embedded using self-assembling peptide RAD16-I with or without GFP-labeled mediastinal adipose tissue-derived progenitor cells (MATPCs). Resulting bioprostheses were implanted over the ischemic myocardium in the swine model of MI (n=8 treated and n=5 control animals). For in vivo electrical impedance spectroscopy (EIS) monitoring, two electrodes were anchored to construct edges, covered by NanoGold particles and connected to an impedance-based implantable device. Histological evaluation was performed to identify and characterize GFP cells on post mortem myocardial sections.RESULTS: Pluripotency, cardiomyogenic and endothelial potential and migratory capacity of porcine-derived MATPCs were demonstrated in vitro. Decellularization protocol efficiency, biodegradability, as well as in vitro biocompatibility after recellularization were also verified. One month after myocardial bioprosthesis implantation, morphometry revealed a 36% reduction in infarct area, Ki67(+)-GFP(+)-MATPCs were found at infarct core and border zones, and bioprosthesis vascularization was confirmed by presence of Griffonia simplicifolia lectin I (GSLI) B4 isolectin(+)-GFP(+)-MATPCs. Electrical impedance measurement at low and high frequencies (10 kHz-100 kHz) allowed online monitoring of scar maturation.CONCLUSIONS: With clinical translation as ultimate goal, this myocardial bioprosthesis holds promise to be a viable candidate for human cardiac repair.

AB - BACKGROUND/OBJECTIVES: The aim of this study was to develop a myocardial bioprosthesis for cardiac repair with an integrated online monitoring system. Myocardial infarction (MI) causes irreversible myocyte loss and scar formation. Tissue engineering to reduce myocardial scar size has been tested with variable success, yet scar formation and modulation by an engineered graft is incompletely characterized.METHODS: Decellularized human pericardium was embedded using self-assembling peptide RAD16-I with or without GFP-labeled mediastinal adipose tissue-derived progenitor cells (MATPCs). Resulting bioprostheses were implanted over the ischemic myocardium in the swine model of MI (n=8 treated and n=5 control animals). For in vivo electrical impedance spectroscopy (EIS) monitoring, two electrodes were anchored to construct edges, covered by NanoGold particles and connected to an impedance-based implantable device. Histological evaluation was performed to identify and characterize GFP cells on post mortem myocardial sections.RESULTS: Pluripotency, cardiomyogenic and endothelial potential and migratory capacity of porcine-derived MATPCs were demonstrated in vitro. Decellularization protocol efficiency, biodegradability, as well as in vitro biocompatibility after recellularization were also verified. One month after myocardial bioprosthesis implantation, morphometry revealed a 36% reduction in infarct area, Ki67(+)-GFP(+)-MATPCs were found at infarct core and border zones, and bioprosthesis vascularization was confirmed by presence of Griffonia simplicifolia lectin I (GSLI) B4 isolectin(+)-GFP(+)-MATPCs. Electrical impedance measurement at low and high frequencies (10 kHz-100 kHz) allowed online monitoring of scar maturation.CONCLUSIONS: With clinical translation as ultimate goal, this myocardial bioprosthesis holds promise to be a viable candidate for human cardiac repair.

KW - Adipose Tissue/cytology

KW - Aged

KW - Animals

KW - Bioprosthesis

KW - Cardiac Surgical Procedures/methods

KW - Cells, Cultured

KW - Female

KW - Humans

KW - Male

KW - Middle Aged

KW - Monitoring, Ambulatory/methods

KW - Myocardial Ischemia/pathology

KW - Online Systems

KW - Pericardium/cytology

KW - Prosthesis Implantation/methods

KW - Swine

U2 - 10.1016/j.ijcard.2014.04.181

DO - 10.1016/j.ijcard.2014.04.181

M3 - Article

SN - 0167-5273

VL - 174

SP - 654

EP - 661

JO - International journal of cardiology

JF - International journal of cardiology

IS - 3

ER -

Online monitoring of myocardial bioprosthesis for cardiac repair

Abstract

UN SDGs

Keywords

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