Neuron-Glia Interactions Increase Neuronal Phenotypes in Tuberous Sclerosis Complex Patient iPSC-Derived Models

Aishwarya G. Nadadhur; Mouhamed Alsaqati; Lisa Gasparotto; Paulien Cornelissen-Steijger; Eline van Hugte; Stephanie Dooves; Adrian J. Harwood; Vivi M. Heine

doi:10.1016/j.stemcr.2018.11.019

Neuron-Glia Interactions Increase Neuronal Phenotypes in Tuberous Sclerosis Complex Patient iPSC-Derived Models

Aishwarya G. Nadadhur
, Mouhamed Alsaqati
, Lisa Gasparotto
, Paulien Cornelissen-Steijger
, Eline van Hugte
, Stephanie Dooves
, Adrian J. Harwood
, Vivi M. Heine^*

^*Corresponding author for this work

Vrije Universiteit Amsterdam
Cardiff University
Department of Functional Genomics, 1081 Amsterdam, Netherlands
Neuroscience and Mental Health Research Institute, CF24 4HQ Cardiff, United Kingdom
Pediatric Neurology, 1081 Amsterdam, Netherlands
Department of Complex Trait Genetics, 1081 Amsterdam, Netherlands

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Nadadhur et al. generated TSC disease models using patient iPSCs. While neuron mono-cultures showed an increase in network activity and dendritic branching, only when co-cultured with oligodendrocytes (OLs), hypertrophy and axonal abnormalities were observed. Neuron-OL interactions, modulated by mTOR regulators, support use of mixed cultures for TSC disease modeling and drug development.

Original language	English
Pages (from-to)	42-56
Journal	Stem cell reports
Volume	12
Issue number	1
DOIs	https://doi.org/10.1016/j.stemcr.2018.11.019
Publication status	Published - 8 Jan 2019

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10.1016/j.stemcr.2018.11.019

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abstract = "Nadadhur et al. generated TSC disease models using patient iPSCs. While neuron mono-cultures showed an increase in network activity and dendritic branching, only when co-cultured with oligodendrocytes (OLs), hypertrophy and axonal abnormalities were observed. Neuron-OL interactions, modulated by mTOR regulators, support use of mixed cultures for TSC disease modeling and drug development.",

author = "\{G. Nadadhur\}, Aishwarya and Mouhamed Alsaqati and Lisa Gasparotto and Paulien Cornelissen-Steijger and \{van Hugte\}, Eline and Stephanie Dooves and Harwood, \{Adrian J.\} and Heine, \{Vivi M.\}",

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doi = "10.1016/j.stemcr.2018.11.019",

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AU - G. Nadadhur, Aishwarya

AU - Alsaqati, Mouhamed

AU - Gasparotto, Lisa

AU - Cornelissen-Steijger, Paulien

AU - van Hugte, Eline

AU - Dooves, Stephanie

AU - Harwood, Adrian J.

AU - Heine, Vivi M.

PY - 2019/1/8

Y1 - 2019/1/8

N2 - Nadadhur et al. generated TSC disease models using patient iPSCs. While neuron mono-cultures showed an increase in network activity and dendritic branching, only when co-cultured with oligodendrocytes (OLs), hypertrophy and axonal abnormalities were observed. Neuron-OL interactions, modulated by mTOR regulators, support use of mixed cultures for TSC disease modeling and drug development.

AB - Nadadhur et al. generated TSC disease models using patient iPSCs. While neuron mono-cultures showed an increase in network activity and dendritic branching, only when co-cultured with oligodendrocytes (OLs), hypertrophy and axonal abnormalities were observed. Neuron-OL interactions, modulated by mTOR regulators, support use of mixed cultures for TSC disease modeling and drug development.

UR - https://www.scopus.com/pages/publications/85059554537

UR - https://www.ncbi.nlm.nih.gov/pubmed/30581017

U2 - 10.1016/j.stemcr.2018.11.019

DO - 10.1016/j.stemcr.2018.11.019

M3 - Article

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SN - 2213-6711

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EP - 56

JO - Stem cell reports

JF - Stem cell reports

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ER -

Neuron-Glia Interactions Increase Neuronal Phenotypes in Tuberous Sclerosis Complex Patient iPSC-Derived Models

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