Menadione partially restores NADH-oxidation and ATP-synthesis in complex I deficient fibroblasts

F. A. Wijburg; N. Feller; C. J. de Groot; R. J. Wanders

Menadione partially restores NADH-oxidation and ATP-synthesis in complex I deficient fibroblasts

F. A. Wijburg
, N. Feller
, C. J. de Groot
, R. J. Wanders

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

In this paper we report our studies on the effects of menadione in cultured fibroblasts treated with rotenone to block complex I. A normalization of the lactate to pyruvate ratio after incubation with glucose, an increased production of 14CO2 from [6-14C]glucose and an increased intra-cellular concentration of ATP was observed in the presence of micromolar concentrations of menadione. These results not only demonstrate the potential value of menadione in complex I deficient patients but also suggest that this system can be used advantageously for the in vitro assessment of therapeutic agents for disorders of the mitochondrial respiratory chain

Original language	English
Pages (from-to)	303-309
Journal	Biochemistry international
Volume	22
Issue number	2
Publication status	Published - 1990

Cite this

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title = "Menadione partially restores NADH-oxidation and ATP-synthesis in complex I deficient fibroblasts",

abstract = "In this paper we report our studies on the effects of menadione in cultured fibroblasts treated with rotenone to block complex I. A normalization of the lactate to pyruvate ratio after incubation with glucose, an increased production of 14CO2 from [6-14C]glucose and an increased intra-cellular concentration of ATP was observed in the presence of micromolar concentrations of menadione. These results not only demonstrate the potential value of menadione in complex I deficient patients but also suggest that this system can be used advantageously for the in vitro assessment of therapeutic agents for disorders of the mitochondrial respiratory chain",

author = "Wijburg, \{F. A.\} and N. Feller and \{de Groot\}, \{C. J.\} and Wanders, \{R. J.\}",

year = "1990",

language = "English",

volume = "22",

pages = "303--309",

journal = "Biochemistry international",

issn = "0158-5231",

publisher = "Academic Press Inc.",

number = "2",

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TY - JOUR

T1 - Menadione partially restores NADH-oxidation and ATP-synthesis in complex I deficient fibroblasts

AU - Wijburg, F. A.

AU - Feller, N.

AU - de Groot, C. J.

AU - Wanders, R. J.

PY - 1990

Y1 - 1990

N2 - In this paper we report our studies on the effects of menadione in cultured fibroblasts treated with rotenone to block complex I. A normalization of the lactate to pyruvate ratio after incubation with glucose, an increased production of 14CO2 from [6-14C]glucose and an increased intra-cellular concentration of ATP was observed in the presence of micromolar concentrations of menadione. These results not only demonstrate the potential value of menadione in complex I deficient patients but also suggest that this system can be used advantageously for the in vitro assessment of therapeutic agents for disorders of the mitochondrial respiratory chain

AB - In this paper we report our studies on the effects of menadione in cultured fibroblasts treated with rotenone to block complex I. A normalization of the lactate to pyruvate ratio after incubation with glucose, an increased production of 14CO2 from [6-14C]glucose and an increased intra-cellular concentration of ATP was observed in the presence of micromolar concentrations of menadione. These results not only demonstrate the potential value of menadione in complex I deficient patients but also suggest that this system can be used advantageously for the in vitro assessment of therapeutic agents for disorders of the mitochondrial respiratory chain

M3 - Article

C2 - 2128595

SN - 0158-5231

VL - 22

SP - 303

EP - 309

JO - Biochemistry international

JF - Biochemistry international

IS - 2

ER -

Menadione partially restores NADH-oxidation and ATP-synthesis in complex I deficient fibroblasts

Abstract

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