Mapping the human DC lineage through the integration of high-dimensional techniques

Peter See; Charles-Antoine Dutertre; Jinmiao Chen; Patrick Günther; Naomi McGovern; Sergio Erdal Irac; Merry Gunawan; Marc Beyer; Kristian Händler; Kaibo Duan; Hermi Rizal Bin Sumatoh; Nicolas Ruffin; Mabel Jouve; Ester Gea-Mallorquí; Raoul C. M. Hennekam; Tony Lim; Chan Chung Yip; Ming Wen; Benoit Malleret; Ivy Low; Nurhidaya Binte Shadan; Charlene Foong Shu Fen; Alicia Tay; Josephine Lum; Francesca Zolezzi; Anis Larbi; Michael Poidinger; Jerry K. Y. Chan; Qingfeng Chen; Laurent Rénia; Muzlifah Haniffa; Philippe Benaroch; Andreas Schlitzer; Joachim L. Schultze; Evan W. Newell; Florent Ginhoux

doi:10.1126/science.aag3009

Mapping the human DC lineage through the integration of high-dimensional techniques

Peter See
, Charles-Antoine Dutertre
, Jinmiao Chen
, Patrick Günther
, Naomi McGovern
, Sergio Erdal Irac
, Merry Gunawan
, Marc Beyer
, Kristian Händler
, Kaibo Duan
, Hermi Rizal Bin Sumatoh
, Nicolas Ruffin
, Mabel Jouve
, Ester Gea-Mallorquí
, Raoul C. M. Hennekam
, Tony Lim
, Chan Chung Yip
, Ming Wen
, Benoit Malleret
, Ivy Low

Nurhidaya Binte Shadan, Charlene Foong Shu Fen, Alicia Tay, Josephine Lum, Francesca Zolezzi, Anis Larbi, Michael Poidinger, Jerry K. Y. Chan, Qingfeng Chen, Laurent Rénia, Muzlifah Haniffa, Philippe Benaroch, Andreas Schlitzer, Joachim L. Schultze, Evan W. Newell, Florent Ginhoux

Research output: Contribution to journal › Article › Academic › peer-review

97 Downloads (Pure)

Abstract

Dendritic cells (DC) are professional antigen-presenting cells that orchestrate immune responses. The human DC population comprises two main functionally specialized lineages, whose origins and differentiation pathways remain incompletely defined. Here, we combine two high-dimensional technologies-single-cell messenger RNA sequencing (scmRNAseq) and cytometry by time-of-flight (CyTOF)-to identify human blood CD123+CD33+CD45RA+ DC precursors (pre-DC). Pre-DC share surface markers with plasmacytoid DC (pDC) but have distinct functional properties that were previously attributed to pDC. Tracing the differentiation of DC from the bone marrow to the peripheral blood revealed that the pre-DC compartment contains distinct lineage-committed subpopulations, including one early uncommitted CD123high pre-DC subset and two CD45RA+CD123low lineage-committed subsets exhibiting functional differences. The discovery of multiple committed pre-DC populations opens promising new avenues for the therapeutic exploitation of DC subset-specific targeting

Original language	English
Pages (from-to)	1044-+
Journal	Science
Volume	356
Issue number	6342
DOIs	https://doi.org/10.1126/science.aag3009
Publication status	Published - 2017

Access to Document

10.1126/science.aag3009

Mapping-the-human-dc-lineage-through-the-integration-of-high-dimensional-techniques.pdfFinal published version, 8.53 MBLicence: Taverne

Cite this

See, P., Dutertre, C.-A., Chen, J., Günther, P., McGovern, N., Irac, S. E., Gunawan, M., Beyer, M., Händler, K., Duan, K., Sumatoh, H. R. B., Ruffin, N., Jouve, M., Gea-Mallorquí, E., Hennekam, R. C. M., Lim, T., Yip, C. C., Wen, M., Malleret, B., ... Ginhoux, F. (2017). Mapping the human DC lineage through the integration of high-dimensional techniques. Science, 356(6342), 1044-+. https://doi.org/10.1126/science.aag3009

@article{4075e434646240f6afc48f4a287a37ae,

title = "Mapping the human DC lineage through the integration of high-dimensional techniques",

abstract = "Dendritic cells (DC) are professional antigen-presenting cells that orchestrate immune responses. The human DC population comprises two main functionally specialized lineages, whose origins and differentiation pathways remain incompletely defined. Here, we combine two high-dimensional technologies-single-cell messenger RNA sequencing (scmRNAseq) and cytometry by time-of-flight (CyTOF)-to identify human blood CD123+CD33+CD45RA+ DC precursors (pre-DC). Pre-DC share surface markers with plasmacytoid DC (pDC) but have distinct functional properties that were previously attributed to pDC. Tracing the differentiation of DC from the bone marrow to the peripheral blood revealed that the pre-DC compartment contains distinct lineage-committed subpopulations, including one early uncommitted CD123high pre-DC subset and two CD45RA+CD123low lineage-committed subsets exhibiting functional differences. The discovery of multiple committed pre-DC populations opens promising new avenues for the therapeutic exploitation of DC subset-specific targeting",

author = "Peter See and Charles-Antoine Dutertre and Jinmiao Chen and Patrick G{\"u}nther and Naomi McGovern and Irac, \{Sergio Erdal\} and Merry Gunawan and Marc Beyer and Kristian H{\"a}ndler and Kaibo Duan and Sumatoh, \{Hermi Rizal Bin\} and Nicolas Ruffin and Mabel Jouve and Ester Gea-Mallorqu{\'i} and Hennekam, \{Raoul C. M.\} and Tony Lim and Yip, \{Chan Chung\} and Ming Wen and Benoit Malleret and Ivy Low and Shadan, \{Nurhidaya Binte\} and Fen, \{Charlene Foong Shu\} and Alicia Tay and Josephine Lum and Francesca Zolezzi and Anis Larbi and Michael Poidinger and Chan, \{Jerry K. Y.\} and Qingfeng Chen and Laurent R{\'e}nia and Muzlifah Haniffa and Philippe Benaroch and Andreas Schlitzer and Schultze, \{Joachim L.\} and Newell, \{Evan W.\} and Florent Ginhoux",

year = "2017",

doi = "10.1126/science.aag3009",

language = "English",

volume = "356",

pages = "1044--+",

journal = "Science",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

number = "6342",

}

See, P, Dutertre, C-A, Chen, J, Günther, P, McGovern, N, Irac, SE, Gunawan, M, Beyer, M, Händler, K, Duan, K, Sumatoh, HRB, Ruffin, N, Jouve, M, Gea-Mallorquí, E, Hennekam, RCM, Lim, T, Yip, CC, Wen, M, Malleret, B, Low, I, Shadan, NB, Fen, CFS, Tay, A, Lum, J, Zolezzi, F, Larbi, A, Poidinger, M, Chan, JKY, Chen, Q, Rénia, L, Haniffa, M, Benaroch, P, Schlitzer, A, Schultze, JL, Newell, EW & Ginhoux, F 2017, 'Mapping the human DC lineage through the integration of high-dimensional techniques', Science, vol. 356, no. 6342, pp. 1044-+. https://doi.org/10.1126/science.aag3009

TY - JOUR

T1 - Mapping the human DC lineage through the integration of high-dimensional techniques

AU - See, Peter

AU - Dutertre, Charles-Antoine

AU - Chen, Jinmiao

AU - Günther, Patrick

AU - McGovern, Naomi

AU - Irac, Sergio Erdal

AU - Gunawan, Merry

AU - Beyer, Marc

AU - Händler, Kristian

AU - Duan, Kaibo

AU - Sumatoh, Hermi Rizal Bin

AU - Ruffin, Nicolas

AU - Jouve, Mabel

AU - Gea-Mallorquí, Ester

AU - Hennekam, Raoul C. M.

AU - Lim, Tony

AU - Yip, Chan Chung

AU - Wen, Ming

AU - Malleret, Benoit

AU - Low, Ivy

AU - Shadan, Nurhidaya Binte

AU - Fen, Charlene Foong Shu

AU - Tay, Alicia

AU - Lum, Josephine

AU - Zolezzi, Francesca

AU - Larbi, Anis

AU - Poidinger, Michael

AU - Chan, Jerry K. Y.

AU - Chen, Qingfeng

AU - Rénia, Laurent

AU - Haniffa, Muzlifah

AU - Benaroch, Philippe

AU - Schlitzer, Andreas

AU - Schultze, Joachim L.

AU - Newell, Evan W.

AU - Ginhoux, Florent

PY - 2017

Y1 - 2017

N2 - Dendritic cells (DC) are professional antigen-presenting cells that orchestrate immune responses. The human DC population comprises two main functionally specialized lineages, whose origins and differentiation pathways remain incompletely defined. Here, we combine two high-dimensional technologies-single-cell messenger RNA sequencing (scmRNAseq) and cytometry by time-of-flight (CyTOF)-to identify human blood CD123+CD33+CD45RA+ DC precursors (pre-DC). Pre-DC share surface markers with plasmacytoid DC (pDC) but have distinct functional properties that were previously attributed to pDC. Tracing the differentiation of DC from the bone marrow to the peripheral blood revealed that the pre-DC compartment contains distinct lineage-committed subpopulations, including one early uncommitted CD123high pre-DC subset and two CD45RA+CD123low lineage-committed subsets exhibiting functional differences. The discovery of multiple committed pre-DC populations opens promising new avenues for the therapeutic exploitation of DC subset-specific targeting

AB - Dendritic cells (DC) are professional antigen-presenting cells that orchestrate immune responses. The human DC population comprises two main functionally specialized lineages, whose origins and differentiation pathways remain incompletely defined. Here, we combine two high-dimensional technologies-single-cell messenger RNA sequencing (scmRNAseq) and cytometry by time-of-flight (CyTOF)-to identify human blood CD123+CD33+CD45RA+ DC precursors (pre-DC). Pre-DC share surface markers with plasmacytoid DC (pDC) but have distinct functional properties that were previously attributed to pDC. Tracing the differentiation of DC from the bone marrow to the peripheral blood revealed that the pre-DC compartment contains distinct lineage-committed subpopulations, including one early uncommitted CD123high pre-DC subset and two CD45RA+CD123low lineage-committed subsets exhibiting functional differences. The discovery of multiple committed pre-DC populations opens promising new avenues for the therapeutic exploitation of DC subset-specific targeting

U2 - 10.1126/science.aag3009

DO - 10.1126/science.aag3009

M3 - Article

C2 - 28473638

SN - 0036-8075

VL - 356

SP - 1044-+

JO - Science

JF - Science

IS - 6342

ER -

Mapping the human DC lineage through the integration of high-dimensional techniques

Abstract

Access to Document

Fingerprint

Cite this