Local irradiation of patient-derived tumors in immunodeficient mice

Amber P. van der Zalm; Sanne Bootsma; Hans M. Rodermond; Arlene L. Oei; Maarten F. Bijlsma

doi:10.1016/j.xpro.2023.102098

Local irradiation of patient-derived tumors in immunodeficient mice

Amber P. van der Zalm
, Sanne Bootsma
, Hans M. Rodermond
, Arlene L. Oei^*
, Maarten F. Bijlsma

^*Corresponding author for this work

Oncode Institute, Amsterdam, the Netherlands
Amsterdam UMC
University of Amsterdam

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Severe combined immunodeficient mice are typically used for xenografting experiments and show reliable tumor engraftment; however, their Prkd^scid mutation renders them highly sensitive to irradiation. Here, we describe a protocol that allows safe local irradiation of tumor xenografts in immunodeficient mice. We detail the steps for the establishment and handling of patient-derived cancer cultures, subcutaneous injection of cancer cells on the mouse hind limb, localized irradiation in mice, tumor monitoring, and tumor characterization via histological and immunohistochemical assessment. For complete details on the use and execution of this protocol, please refer to Dings et al. (2022).¹

Original language	English
Article number	102098
Journal	STAR Protocols
Volume	4
Issue number	1
DOIs	https://doi.org/10.1016/j.xpro.2023.102098
Publication status	Published - 17 Mar 2023

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Cancer
Cell Culture
Health Sciences
Microscopy
Model Organisms

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10.1016/j.xpro.2023.102098

Local-irradiation-of-patient-derived-tumors-in-immunodeficient-mice.pdfFinal published version, 3.96 MBLicence: CC BY

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title = "Local irradiation of patient-derived tumors in immunodeficient mice",

abstract = "Severe combined immunodeficient mice are typically used for xenografting experiments and show reliable tumor engraftment; however, their Prkdscid mutation renders them highly sensitive to irradiation. Here, we describe a protocol that allows safe local irradiation of tumor xenografts in immunodeficient mice. We detail the steps for the establishment and handling of patient-derived cancer cultures, subcutaneous injection of cancer cells on the mouse hind limb, localized irradiation in mice, tumor monitoring, and tumor characterization via histological and immunohistochemical assessment. For complete details on the use and execution of this protocol, please refer to Dings et al. (2022).1",

keywords = "Cancer, Cell Culture, Health Sciences, Microscopy, Model Organisms",

author = "\{van der Zalm\}, \{Amber P.\} and Sanne Bootsma and Rodermond, \{Hans M.\} and Oei, \{Arlene L.\} and Bijlsma, \{Maarten F.\}",

note = "Funding Information: M.F.B. has received research funding from Celgene, Frame Therapeutics, and Lead Pharma and has acted as a consultant to Servier. Funding Information: This work was supported by KWF Dutch Cancer Society to M.F.B. (project number 10992 ). We would like to thank Cynthia Waasdorp, Marieke ten Brink, and the staff of the animal facility for technical assistance. We would like to acknowledge Eric van der Zalm and Gerbrand de Jong for creating the technical drawing and providing the files of the mice radiation setup. Publisher Copyright: {\textcopyright} 2023 The Author(s)",

year = "2023",

month = mar,

day = "17",

doi = "10.1016/j.xpro.2023.102098",

language = "English",

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TY - JOUR

T1 - Local irradiation of patient-derived tumors in immunodeficient mice

AU - van der Zalm, Amber P.

AU - Bootsma, Sanne

AU - Rodermond, Hans M.

AU - Oei, Arlene L.

AU - Bijlsma, Maarten F.

N1 - Funding Information: M.F.B. has received research funding from Celgene, Frame Therapeutics, and Lead Pharma and has acted as a consultant to Servier. Funding Information: This work was supported by KWF Dutch Cancer Society to M.F.B. (project number 10992 ). We would like to thank Cynthia Waasdorp, Marieke ten Brink, and the staff of the animal facility for technical assistance. We would like to acknowledge Eric van der Zalm and Gerbrand de Jong for creating the technical drawing and providing the files of the mice radiation setup. Publisher Copyright: © 2023 The Author(s)

PY - 2023/3/17

Y1 - 2023/3/17

N2 - Severe combined immunodeficient mice are typically used for xenografting experiments and show reliable tumor engraftment; however, their Prkdscid mutation renders them highly sensitive to irradiation. Here, we describe a protocol that allows safe local irradiation of tumor xenografts in immunodeficient mice. We detail the steps for the establishment and handling of patient-derived cancer cultures, subcutaneous injection of cancer cells on the mouse hind limb, localized irradiation in mice, tumor monitoring, and tumor characterization via histological and immunohistochemical assessment. For complete details on the use and execution of this protocol, please refer to Dings et al. (2022).1

AB - Severe combined immunodeficient mice are typically used for xenografting experiments and show reliable tumor engraftment; however, their Prkdscid mutation renders them highly sensitive to irradiation. Here, we describe a protocol that allows safe local irradiation of tumor xenografts in immunodeficient mice. We detail the steps for the establishment and handling of patient-derived cancer cultures, subcutaneous injection of cancer cells on the mouse hind limb, localized irradiation in mice, tumor monitoring, and tumor characterization via histological and immunohistochemical assessment. For complete details on the use and execution of this protocol, please refer to Dings et al. (2022).1

KW - Cancer

KW - Cell Culture

KW - Health Sciences

KW - Microscopy

KW - Model Organisms

UR - https://www.scopus.com/pages/publications/85147594713

U2 - 10.1016/j.xpro.2023.102098

DO - 10.1016/j.xpro.2023.102098

M3 - Article

C2 - 36825807

SN - 2666-1667

VL - 4

JO - STAR Protocols

JF - STAR Protocols

IS - 1

M1 - 102098

ER -

Local irradiation of patient-derived tumors in immunodeficient mice

Abstract

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Keywords

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