Lipid droplets: a new player in colorectal cancer stem cells unveiled by spectroscopic imaging

Luca Tirinato; Carlo Liberale; Simone Di Franco; Patrizio Candeloro; Antonina Benfante; Rosanna La Rocca; Lisette Potze; Roberto Marotta; Roberta Ruffilli; Vijayakumar P. Rajamanickam; Mario Malerba; Francesco de Angelis; Andrea Falqui; Ennio Carbone; Matilde Todaro; Jan Paul Medema; Giorgio Stassi; Enzo Di Fabrizio

doi:10.1002/stem.1837

Lipid droplets: a new player in colorectal cancer stem cells unveiled by spectroscopic imaging

Luca Tirinato, Carlo Liberale, Simone Di Franco, Patrizio Candeloro, Antonina Benfante, Rosanna La Rocca, Lisette Potze, Roberto Marotta, Roberta Ruffilli, Vijayakumar P. Rajamanickam, Mario Malerba, Francesco de Angelis, Andrea Falqui, Ennio Carbone, Matilde Todaro, Jan Paul Medema, Giorgio Stassi, Enzo Di Fabrizio

Research output: Contribution to journal › Article › Academic › peer-review

189 Citations (Scopus)

Abstract

The cancer stem cell (CSC) model is describing tumors as a hierarchical organized system and CSCs are suggested to be responsible for cancer recurrence after therapy. The identification of specific markers of CSCs is therefore of paramount importance. Here, we show that high levels of lipid droplets (LDs) are a distinctive mark of CSCs in colorectal (CR) cancer. This increased lipid content was clearly revealed by label-free Raman spectroscopy and it directly correlates with well-accepted CR-CSC markers as CD133 and Wnt pathway activity. By xenotransplantation experiments, we have finally demonstrated that CR-CSCs overexpressing LDs retain most tumorigenic potential. A relevant conceptual advance in this work is the demonstration that a cellular organelle, the LD, is a signature of CSCs, in addition to molecular markers. A further functional characterization of LDs could lead soon to design new target therapies against CR-CSCs

Original language	English
Pages (from-to)	35-44
Journal	Stem cells (Dayton, Ohio)
Volume	33
Issue number	1
DOIs	https://doi.org/10.1002/stem.1837
Publication status	Published - 2015

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10.1002/stem.1837

Cite this

Tirinato, L., Liberale, C., Di Franco, S., Candeloro, P., Benfante, A., La Rocca, R., Potze, L., Marotta, R., Ruffilli, R., Rajamanickam, V. P., Malerba, M., de Angelis, F., Falqui, A., Carbone, E., Todaro, M., Medema, J. P., Stassi, G., & Di Fabrizio, E. (2015). Lipid droplets: a new player in colorectal cancer stem cells unveiled by spectroscopic imaging. Stem cells (Dayton, Ohio), 33(1), 35-44. https://doi.org/10.1002/stem.1837

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title = "Lipid droplets: a new player in colorectal cancer stem cells unveiled by spectroscopic imaging",

abstract = "The cancer stem cell (CSC) model is describing tumors as a hierarchical organized system and CSCs are suggested to be responsible for cancer recurrence after therapy. The identification of specific markers of CSCs is therefore of paramount importance. Here, we show that high levels of lipid droplets (LDs) are a distinctive mark of CSCs in colorectal (CR) cancer. This increased lipid content was clearly revealed by label-free Raman spectroscopy and it directly correlates with well-accepted CR-CSC markers as CD133 and Wnt pathway activity. By xenotransplantation experiments, we have finally demonstrated that CR-CSCs overexpressing LDs retain most tumorigenic potential. A relevant conceptual advance in this work is the demonstration that a cellular organelle, the LD, is a signature of CSCs, in addition to molecular markers. A further functional characterization of LDs could lead soon to design new target therapies against CR-CSCs",

author = "Luca Tirinato and Carlo Liberale and {Di Franco}, Simone and Patrizio Candeloro and Antonina Benfante and {La Rocca}, Rosanna and Lisette Potze and Roberto Marotta and Roberta Ruffilli and Rajamanickam, {Vijayakumar P.} and Mario Malerba and {de Angelis}, Francesco and Andrea Falqui and Ennio Carbone and Matilde Todaro and Medema, {Jan Paul} and Giorgio Stassi and {Di Fabrizio}, Enzo",

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Tirinato, L, Liberale, C, Di Franco, S, Candeloro, P, Benfante, A, La Rocca, R, Potze, L, Marotta, R, Ruffilli, R, Rajamanickam, VP, Malerba, M, de Angelis, F, Falqui, A, Carbone, E, Todaro, M, Medema, JP, Stassi, G & Di Fabrizio, E 2015, 'Lipid droplets: a new player in colorectal cancer stem cells unveiled by spectroscopic imaging', Stem cells (Dayton, Ohio), vol. 33, no. 1, pp. 35-44. https://doi.org/10.1002/stem.1837

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T1 - Lipid droplets: a new player in colorectal cancer stem cells unveiled by spectroscopic imaging

AU - Tirinato, Luca

AU - Liberale, Carlo

AU - Di Franco, Simone

AU - Candeloro, Patrizio

AU - Benfante, Antonina

AU - La Rocca, Rosanna

AU - Potze, Lisette

AU - Marotta, Roberto

AU - Ruffilli, Roberta

AU - Rajamanickam, Vijayakumar P.

AU - Malerba, Mario

AU - de Angelis, Francesco

AU - Falqui, Andrea

AU - Carbone, Ennio

AU - Todaro, Matilde

AU - Medema, Jan Paul

AU - Stassi, Giorgio

AU - Di Fabrizio, Enzo

PY - 2015

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N2 - The cancer stem cell (CSC) model is describing tumors as a hierarchical organized system and CSCs are suggested to be responsible for cancer recurrence after therapy. The identification of specific markers of CSCs is therefore of paramount importance. Here, we show that high levels of lipid droplets (LDs) are a distinctive mark of CSCs in colorectal (CR) cancer. This increased lipid content was clearly revealed by label-free Raman spectroscopy and it directly correlates with well-accepted CR-CSC markers as CD133 and Wnt pathway activity. By xenotransplantation experiments, we have finally demonstrated that CR-CSCs overexpressing LDs retain most tumorigenic potential. A relevant conceptual advance in this work is the demonstration that a cellular organelle, the LD, is a signature of CSCs, in addition to molecular markers. A further functional characterization of LDs could lead soon to design new target therapies against CR-CSCs

AB - The cancer stem cell (CSC) model is describing tumors as a hierarchical organized system and CSCs are suggested to be responsible for cancer recurrence after therapy. The identification of specific markers of CSCs is therefore of paramount importance. Here, we show that high levels of lipid droplets (LDs) are a distinctive mark of CSCs in colorectal (CR) cancer. This increased lipid content was clearly revealed by label-free Raman spectroscopy and it directly correlates with well-accepted CR-CSC markers as CD133 and Wnt pathway activity. By xenotransplantation experiments, we have finally demonstrated that CR-CSCs overexpressing LDs retain most tumorigenic potential. A relevant conceptual advance in this work is the demonstration that a cellular organelle, the LD, is a signature of CSCs, in addition to molecular markers. A further functional characterization of LDs could lead soon to design new target therapies against CR-CSCs

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