Laser microdissection for spatial proteomics of postmortem brain tissue

David C. Hondius; Jeroen J.M. Hoozemans; Annemieke J.M. Rozemuller; August B. Smit

doi:10.1007/978-1-4939-9662-9_4

Laser microdissection for spatial proteomics of postmortem brain tissue

David C. Hondius^*
, Jeroen J.M. Hoozemans
, Annemieke J.M. Rozemuller
, August B. Smit

^*Corresponding author for this work

Vrije Universiteit Amsterdam

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic › peer-review

Abstract

Quantitative data of the proteome is highly valuable for providing unbiased information on protein expression changes related to disease or identifying related biomarkers. In brain diseases the affected area can be small and pathogenic events can be related to a specific cell type in an otherwise heterogeneous tissue type. An emerging approach dedicated to analysing this type of samples is laser microdissection (LMD) combined with LC-MS/MS into a single workflow. In this chapter, we describe two LMD methods for isolating tissue at the level of a small area and individual cells suitable for subsequent LC-MS/MS analysis.

Original language	English
Title of host publication	Neuromethods
Publisher	Humana Press Inc.
Pages	43-53
Number of pages	11
DOIs	https://doi.org/10.1007/978-1-4939-9662-9_4
Publication status	Published - 1 Jan 2019

Publication series

Name	Neuromethods
Volume	146
ISSN (Print)	0893-2336
ISSN (Electronic)	1940-6045

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Human brain
Immunohistochemistry
Laser capture
Laser microdissection
Mass spectrometry
Postmortem tissue
Proteomics
Single cell

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10.1007/978-1-4939-9662-9_4

Cite this

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abstract = "Quantitative data of the proteome is highly valuable for providing unbiased information on protein expression changes related to disease or identifying related biomarkers. In brain diseases the affected area can be small and pathogenic events can be related to a specific cell type in an otherwise heterogeneous tissue type. An emerging approach dedicated to analysing this type of samples is laser microdissection (LMD) combined with LC-MS/MS into a single workflow. In this chapter, we describe two LMD methods for isolating tissue at the level of a small area and individual cells suitable for subsequent LC-MS/MS analysis.",

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AU - Hoozemans, Jeroen J.M.

AU - Rozemuller, Annemieke J.M.

AU - Smit, August B.

PY - 2019/1/1

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AB - Quantitative data of the proteome is highly valuable for providing unbiased information on protein expression changes related to disease or identifying related biomarkers. In brain diseases the affected area can be small and pathogenic events can be related to a specific cell type in an otherwise heterogeneous tissue type. An emerging approach dedicated to analysing this type of samples is laser microdissection (LMD) combined with LC-MS/MS into a single workflow. In this chapter, we describe two LMD methods for isolating tissue at the level of a small area and individual cells suitable for subsequent LC-MS/MS analysis.

KW - Human brain

KW - Immunohistochemistry

KW - Laser capture

KW - Laser microdissection

KW - Mass spectrometry

KW - Postmortem tissue

KW - Proteomics

KW - Single cell

UR - https://www.scopus.com/pages/publications/85070536637

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DO - 10.1007/978-1-4939-9662-9_4

M3 - Chapter

T3 - Neuromethods

SP - 43

EP - 53

BT - Neuromethods

PB - Humana Press Inc.

ER -

Laser microdissection for spatial proteomics of postmortem brain tissue

Abstract

Publication series

UN SDGs

Keywords

Access to Document

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