Isolation, propagation, and titration of human immunodeficiency virus type 1 from peripheral blood of infected individuals

Hanneke Schuitemaker; Neeltje A. Kootstra

doi:10.1385/1-59259-907-9:017

Isolation, propagation, and titration of human immunodeficiency virus type 1 from peripheral blood of infected individuals

Hanneke Schuitemaker
, Neeltje A. Kootstra

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

HIV-1 can be isolated from peripheral blood mononuclear cells and is easily propagated on primary cells in vitro. Here we describe the method for bulk isolation of the HIV-1 quasispecies and a limiting dilution virus isolation protocol by which single coexisting clones can be obtained. In addition, methods for propagation and titration of HIV-1 are provided

Original language	English
Pages (from-to)	17-24
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	304
DOIs	https://doi.org/10.1385/1-59259-907-9:017
Publication status	Published - 2005

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SDG 3 Good Health and Well-being

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10.1385/1-59259-907-9:017

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title = "Isolation, propagation, and titration of human immunodeficiency virus type 1 from peripheral blood of infected individuals",

abstract = "HIV-1 can be isolated from peripheral blood mononuclear cells and is easily propagated on primary cells in vitro. Here we describe the method for bulk isolation of the HIV-1 quasispecies and a limiting dilution virus isolation protocol by which single coexisting clones can be obtained. In addition, methods for propagation and titration of HIV-1 are provided",

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AU - Kootstra, Neeltje A.

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AB - HIV-1 can be isolated from peripheral blood mononuclear cells and is easily propagated on primary cells in vitro. Here we describe the method for bulk isolation of the HIV-1 quasispecies and a limiting dilution virus isolation protocol by which single coexisting clones can be obtained. In addition, methods for propagation and titration of HIV-1 are provided

U2 - 10.1385/1-59259-907-9:017

DO - 10.1385/1-59259-907-9:017

M3 - Article

C2 - 16061963

SN - 1064-3745

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JO - Methods in molecular biology (Clifton, N.J.)

JF - Methods in molecular biology (Clifton, N.J.)

ER -

Isolation, propagation, and titration of human immunodeficiency virus type 1 from peripheral blood of infected individuals

Abstract

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