Isolation and propagation of colon cancer stem cells

Pramudita R. Prasetyanti; Cheryl Zimberlin; Felipe de Sousa E Melo; Jan Paul Medema

doi:10.1007/978-1-62703-508-8_21

Isolation and propagation of colon cancer stem cells

Pramudita R. Prasetyanti, Cheryl Zimberlin, Felipe de Sousa E Melo, Jan Paul Medema

Research output: Contribution to journal › Article › Academic › peer-review

17 Citations (Scopus)

Abstract

The design of tissue culture conditions that faithfully reproduce the characteristics of cells in their native environment remains one of the main challenges of cancer stem cell (CSC) biology. Here we describe a detailed methodology for the isolation and expansion of both human colon CSCs and mouse intestinal adenoma together with a brief differentiation and coculture method that proved to be valuable to study the concept of CSCs plasticity

Original language	English
Pages (from-to)	247-259
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	1035
DOIs	https://doi.org/10.1007/978-1-62703-508-8_21
Publication status	Published - 2013

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10.1007/978-1-62703-508-8_21

Cite this

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title = "Isolation and propagation of colon cancer stem cells",

abstract = "The design of tissue culture conditions that faithfully reproduce the characteristics of cells in their native environment remains one of the main challenges of cancer stem cell (CSC) biology. Here we describe a detailed methodology for the isolation and expansion of both human colon CSCs and mouse intestinal adenoma together with a brief differentiation and coculture method that proved to be valuable to study the concept of CSCs plasticity",

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AU - Zimberlin, Cheryl

AU - de Sousa E Melo, Felipe

AU - Medema, Jan Paul

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AB - The design of tissue culture conditions that faithfully reproduce the characteristics of cells in their native environment remains one of the main challenges of cancer stem cell (CSC) biology. Here we describe a detailed methodology for the isolation and expansion of both human colon CSCs and mouse intestinal adenoma together with a brief differentiation and coculture method that proved to be valuable to study the concept of CSCs plasticity

U2 - 10.1007/978-1-62703-508-8_21

DO - 10.1007/978-1-62703-508-8_21

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JO - Methods in molecular biology (Clifton, N.J.)

JF - Methods in molecular biology (Clifton, N.J.)

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