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In Vivo Visualization and Quantification of Mitochondrial Morphology in C. elegans

  • R. de Boer
  • , R. L. Smith
  • , W. H. de Vos
  • , E. M. M. Manders
  • , H. van der Spek*
  • *Corresponding author for this work
  • University of Amsterdam
  • Amsterdam UMC - University of Amsterdam
  • University of Antwerp
  • Ghent University

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

Abstract

Caenorhabditis elegans is a highly versatile model system, intensively used for functional, genetic, cytometric, and integrative studies. Due to its simplicity and large muscle cell number, C. elegans has frequently been used to study mitochondrial deficiencies caused by disease or drug toxicity. Here we describe a robust and efficient method to visualize and quantify mitochondrial morphology in vivo. This method has many practical and technical advantages above traditional (manual) methods and provides a comprehensive analysis of mitochondrial morphology.
Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages397-407
Number of pages11
Volume2276
DOIs
Publication statusPublished - 2021
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume2276
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Caenorhabditis elegans
  • Confocal microscopy
  • Cytometry
  • GFP
  • Image analysis
  • Mitochondria
  • Morphology

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