Identification of a new human coronavirus

Lia van der Hoek; Krzysztof Pyrc; Maarten F. Jebbink; Wilma Vermeulen-Oost; Ron J. M. Berkhout; Katja C. Wolthers; Pauline M. E. Wertheim-van Dillen; Jos Kaandorp; Joke Spaargaren; Ben Berkhout

doi:10.1038/nm1024

Identification of a new human coronavirus

Lia van der Hoek
, Krzysztof Pyrc
, Maarten F. Jebbink
, Wilma Vermeulen-Oost
, Ron J. M. Berkhout
, Katja C. Wolthers
, Pauline M. E. Wertheim-van Dillen
, Jos Kaandorp
, Joke Spaargaren
, Ben Berkhout

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Three human coronaviruses are known to exist: human coronavirus 229E (HCoV-229E), HCoV-OC43 and severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV). Here we report the identification of a fourth human coronavirus, HCoV-NL63, using a new method of virus discovery. The virus was isolated from a 7-month-old child suffering from bronchiolitis and conjunctivitis. The complete genome sequence indicates that this virus is not a recombinant, but rather a new group 1 coronavirus. The in vitro host cell range of HCoV-NL63 is notable because it replicates on tertiary monkey kidney cells and the monkey kidney LLC-MK2 cell line. The viral genome contains distinctive features, including a unique N-terminal fragment within the spike protein. Screening of clinical specimens from individuals suffering from respiratory illness identified seven additional HCoV-NL63-infected individuals, indicating that the virus was widely spread within the human population

Original language	English
Pages (from-to)	368-373
Journal	Nature medicine
Volume	10
Issue number	4
DOIs	https://doi.org/10.1038/nm1024
Publication status	Published - 2004

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This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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10.1038/nm1024

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title = "Identification of a new human coronavirus",

abstract = "Three human coronaviruses are known to exist: human coronavirus 229E (HCoV-229E), HCoV-OC43 and severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV). Here we report the identification of a fourth human coronavirus, HCoV-NL63, using a new method of virus discovery. The virus was isolated from a 7-month-old child suffering from bronchiolitis and conjunctivitis. The complete genome sequence indicates that this virus is not a recombinant, but rather a new group 1 coronavirus. The in vitro host cell range of HCoV-NL63 is notable because it replicates on tertiary monkey kidney cells and the monkey kidney LLC-MK2 cell line. The viral genome contains distinctive features, including a unique N-terminal fragment within the spike protein. Screening of clinical specimens from individuals suffering from respiratory illness identified seven additional HCoV-NL63-infected individuals, indicating that the virus was widely spread within the human population",

author = "\{van der Hoek\}, Lia and Krzysztof Pyrc and Jebbink, \{Maarten F.\} and Wilma Vermeulen-Oost and Berkhout, \{Ron J. M.\} and Wolthers, \{Katja C.\} and \{Wertheim-van Dillen\}, \{Pauline M. E.\} and Jos Kaandorp and Joke Spaargaren and Ben Berkhout",

year = "2004",

doi = "10.1038/nm1024",

language = "English",

volume = "10",

pages = "368--373",

journal = "Nature medicine",

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T1 - Identification of a new human coronavirus

AU - van der Hoek, Lia

AU - Pyrc, Krzysztof

AU - Jebbink, Maarten F.

AU - Vermeulen-Oost, Wilma

AU - Berkhout, Ron J. M.

AU - Wolthers, Katja C.

AU - Wertheim-van Dillen, Pauline M. E.

AU - Kaandorp, Jos

AU - Spaargaren, Joke

AU - Berkhout, Ben

PY - 2004

Y1 - 2004

N2 - Three human coronaviruses are known to exist: human coronavirus 229E (HCoV-229E), HCoV-OC43 and severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV). Here we report the identification of a fourth human coronavirus, HCoV-NL63, using a new method of virus discovery. The virus was isolated from a 7-month-old child suffering from bronchiolitis and conjunctivitis. The complete genome sequence indicates that this virus is not a recombinant, but rather a new group 1 coronavirus. The in vitro host cell range of HCoV-NL63 is notable because it replicates on tertiary monkey kidney cells and the monkey kidney LLC-MK2 cell line. The viral genome contains distinctive features, including a unique N-terminal fragment within the spike protein. Screening of clinical specimens from individuals suffering from respiratory illness identified seven additional HCoV-NL63-infected individuals, indicating that the virus was widely spread within the human population

AB - Three human coronaviruses are known to exist: human coronavirus 229E (HCoV-229E), HCoV-OC43 and severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV). Here we report the identification of a fourth human coronavirus, HCoV-NL63, using a new method of virus discovery. The virus was isolated from a 7-month-old child suffering from bronchiolitis and conjunctivitis. The complete genome sequence indicates that this virus is not a recombinant, but rather a new group 1 coronavirus. The in vitro host cell range of HCoV-NL63 is notable because it replicates on tertiary monkey kidney cells and the monkey kidney LLC-MK2 cell line. The viral genome contains distinctive features, including a unique N-terminal fragment within the spike protein. Screening of clinical specimens from individuals suffering from respiratory illness identified seven additional HCoV-NL63-infected individuals, indicating that the virus was widely spread within the human population

U2 - 10.1038/nm1024

DO - 10.1038/nm1024

M3 - Article

C2 - 15034574

SN - 1078-8956

VL - 10

SP - 368

EP - 373

JO - Nature medicine

JF - Nature medicine

IS - 4

ER -

Identification of a new human coronavirus

Abstract

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