TY - JOUR
T1 - Human whole-blood NAD+ levels do not vary with age or lifestyle interventions
AU - Trętowicz, Maria M.
AU - Scantlebery, Angelique M. L.
AU - Schomakers, Bauke V.
AU - Eroğlu, Kaan D.
AU - van Weeghel, Michel
AU - Spek, Vera
AU - Vinten, Kasper T.
AU - Legon, Luc
AU - Coskun, Evrim
AU - Millan-Domingo, Fernando
AU - Olaso-Gonzalez, Gloria
AU - Gomez-Cabrera, Maria Carmen
AU - Montoro-García, Silvia
AU - Noguera-Navarro, Clara
AU - van Kuilenburg, André B. P.
AU - Moco, Sofia
AU - van Hattum, Juliette C.
AU - Jørstad, Harald T.
AU - Benali, Mohammed
AU - van der Helder, Jantine
AU - Biersteker, Esmee J. M.
AU - Muniandy, Maheswary
AU - Pietiläinen, Kirsi H.
AU - Pirinen, Eija
AU - Slagboom, P. Eline
AU - Beekman, Marian
AU - Deelen, Joris
AU - Zapata-Pérez, Rubén
AU - Weijs, Peter J. M.
AU - Tieland, Michael
AU - Janssens, Georges E.
AU - Houtkooper, Riekelt H.
N1 - Publisher Copyright:
© The Author(s), under exclusive licence to Springer Nature Limited 2026.
PY - 2026/6
Y1 - 2026/6
N2 - Nicotinamide adenine dinucleotide (NAD+) levels in blood and tissues are widely proposed to decline with age, yet evidence in human blood is inconsistent. Using a rigorously validated ultra-high-performance liquid chromatography coupled with high-resolution mass spectrometry system that accounts for real-world analytical variability, we quantify NAD+ across seven independent human cohorts. We find that whole-blood NAD+ levels remain remarkably stable with age and across lifestyle interventions, but change in response to nicotinamide riboside supplementation, as expected. Our results challenge the utility of blood NAD+ levels as a biomarker of ageing or lifestyle factors.
AB - Nicotinamide adenine dinucleotide (NAD+) levels in blood and tissues are widely proposed to decline with age, yet evidence in human blood is inconsistent. Using a rigorously validated ultra-high-performance liquid chromatography coupled with high-resolution mass spectrometry system that accounts for real-world analytical variability, we quantify NAD+ across seven independent human cohorts. We find that whole-blood NAD+ levels remain remarkably stable with age and across lifestyle interventions, but change in response to nicotinamide riboside supplementation, as expected. Our results challenge the utility of blood NAD+ levels as a biomarker of ageing or lifestyle factors.
UR - https://www.scopus.com/pages/publications/105039284607
U2 - 10.1038/s42255-026-01537-5
DO - 10.1038/s42255-026-01537-5
M3 - Article
C2 - 42135539
SN - 2522-5812
VL - 8
SP - 1282
EP - 1290
JO - Nature Metabolism
JF - Nature Metabolism
IS - 6
ER -