Human IgE does not bind to human FcRn

Maximilian Brinkhaus; Elvera J van der Kooi; Arthur E H Bentlage; Pleuni Ooijevaar-de Heer; Ninotska I L Derksen; Theo Rispens; Gestur Vidarsson

doi:10.1038/s41598-021-03852-1

Human IgE does not bind to human FcRn

Maximilian Brinkhaus
, Elvera J van der Kooi
, Arthur E H Bentlage
, Pleuni Ooijevaar-de Heer
, Ninotska I L Derksen
, Theo Rispens
, Gestur Vidarsson

Sanquin Research, Department of Experimental Immunohematology, Amsterdam and Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam, Amsterdam, the Netherlands
Department of Immunopathology, Sanquin Blood Supply, Division Research and Landsteiner Laboratory of the Academic Medical Center, University of Amsterdam,, Amsterdam, The Netherlands

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

The neonatal Fc receptor (FcRn) is known to mediate placental transfer of IgG from mother to unborn. IgE is widely known for triggering immune responses to environmental antigens. Recent evidence suggests FcRn-mediated transplacental passage of IgE during pregnancy. However, direct interaction of FcRn and IgE was not investigated. Here, we compared binding of human IgE and IgG variants to recombinant soluble human FcRn with β2-microglobulin (sFcRn) in surface plasmon resonance (SPR) at pH 7.4 and pH 6.0. No interaction was found between human IgE and human sFcRn. These results imply that FcRn can only transport IgE indirectly, and thereby possibly transfer allergenic sensitivity from mother to fetus.

Original language	English
Article number	62
Pages (from-to)	62
Journal	Scientific reports
Volume	12
Issue number	1
DOIs	https://doi.org/10.1038/s41598-021-03852-1
Publication status	Published - Dec 2022

Keywords

Biological Transport
Female
Histocompatibility Antigens Class I/metabolism
Humans
Hydrogen-Ion Concentration
Immunoglobulin E/metabolism
Immunoglobulin G/metabolism
Maternal-Fetal Exchange
Placenta/metabolism
Pregnancy
Protein Binding
Receptors, Fc/metabolism
Surface Plasmon Resonance
beta 2-Microglobulin/metabolism

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title = "Human IgE does not bind to human FcRn",

abstract = "The neonatal Fc receptor (FcRn) is known to mediate placental transfer of IgG from mother to unborn. IgE is widely known for triggering immune responses to environmental antigens. Recent evidence suggests FcRn-mediated transplacental passage of IgE during pregnancy. However, direct interaction of FcRn and IgE was not investigated. Here, we compared binding of human IgE and IgG variants to recombinant soluble human FcRn with β2-microglobulin (sFcRn) in surface plasmon resonance (SPR) at pH 7.4 and pH 6.0. No interaction was found between human IgE and human sFcRn. These results imply that FcRn can only transport IgE indirectly, and thereby possibly transfer allergenic sensitivity from mother to fetus.",

keywords = "Biological Transport, Female, Histocompatibility Antigens Class I/metabolism, Humans, Hydrogen-Ion Concentration, Immunoglobulin E/metabolism, Immunoglobulin G/metabolism, Maternal-Fetal Exchange, Placenta/metabolism, Pregnancy, Protein Binding, Receptors, Fc/metabolism, Surface Plasmon Resonance, beta 2-Microglobulin/metabolism",

author = "Maximilian Brinkhaus and \{van der Kooi\}, \{Elvera J\} and Bentlage, \{Arthur E H\} and \{Ooijevaar-de Heer\}, Pleuni and Derksen, \{Ninotska I L\} and Theo Rispens and Gestur Vidarsson",

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year = "2022",

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doi = "10.1038/s41598-021-03852-1",

language = "English",

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T1 - Human IgE does not bind to human FcRn

AU - Brinkhaus, Maximilian

AU - van der Kooi, Elvera J

AU - Bentlage, Arthur E H

AU - Ooijevaar-de Heer, Pleuni

AU - Derksen, Ninotska I L

AU - Rispens, Theo

AU - Vidarsson, Gestur

PY - 2022/12

Y1 - 2022/12

N2 - The neonatal Fc receptor (FcRn) is known to mediate placental transfer of IgG from mother to unborn. IgE is widely known for triggering immune responses to environmental antigens. Recent evidence suggests FcRn-mediated transplacental passage of IgE during pregnancy. However, direct interaction of FcRn and IgE was not investigated. Here, we compared binding of human IgE and IgG variants to recombinant soluble human FcRn with β2-microglobulin (sFcRn) in surface plasmon resonance (SPR) at pH 7.4 and pH 6.0. No interaction was found between human IgE and human sFcRn. These results imply that FcRn can only transport IgE indirectly, and thereby possibly transfer allergenic sensitivity from mother to fetus.

AB - The neonatal Fc receptor (FcRn) is known to mediate placental transfer of IgG from mother to unborn. IgE is widely known for triggering immune responses to environmental antigens. Recent evidence suggests FcRn-mediated transplacental passage of IgE during pregnancy. However, direct interaction of FcRn and IgE was not investigated. Here, we compared binding of human IgE and IgG variants to recombinant soluble human FcRn with β2-microglobulin (sFcRn) in surface plasmon resonance (SPR) at pH 7.4 and pH 6.0. No interaction was found between human IgE and human sFcRn. These results imply that FcRn can only transport IgE indirectly, and thereby possibly transfer allergenic sensitivity from mother to fetus.

KW - Biological Transport

KW - Female

KW - Histocompatibility Antigens Class I/metabolism

KW - Humans

KW - Hydrogen-Ion Concentration

KW - Immunoglobulin E/metabolism

KW - Immunoglobulin G/metabolism

KW - Maternal-Fetal Exchange

KW - Placenta/metabolism

KW - Pregnancy

KW - Protein Binding

KW - Receptors, Fc/metabolism

KW - Surface Plasmon Resonance

KW - beta 2-Microglobulin/metabolism

UR - https://www.scopus.com/pages/publications/85122541965

U2 - 10.1038/s41598-021-03852-1

DO - 10.1038/s41598-021-03852-1

M3 - Article

SN - 2045-2322

VL - 12

SP - 62

JO - Scientific reports

JF - Scientific reports

IS - 1

M1 - 62

ER -

Human IgE does not bind to human FcRn

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