TY - JOUR
T1 - Gene expression kinetics in Sepsis After Cardiac Surgery (SACS)
T2 - a multicentric prospective observational study
AU - Radice, Rosa Paola
AU - Martelli, Giuseppe
AU - D’Amora, Mauro
AU - Dambruoso, Pierpaolo
AU - Paparella, Domenico
AU - Mandarano, Raffaele
AU - Olivo, Giuseppe
AU - Scolaro, Massimo
AU - Sarubbi, Domenico
AU - Strumia, Alessandro
AU - Calabrese, Maria
AU - Scapigliati, Andrea
AU - Greco, Francesco
AU - Nardi, Mary
AU - Beccaria, Stefano
AU - Costamagna, Andrea
AU - Brazzi, Luca
AU - Rizzo, Stefano
AU - Pierani, Tommaso
AU - Antoniucci, Maria Enrica
AU - Vignale, Rosaria
AU - Savino, Antonio Ferdinando
AU - Bevilacqua, Sergio
AU - Rotunno, Ezia
AU - Abelardo, Domenico
AU - SIAARTI Study Group
AU - Raimondo, Pasquale
AU - Paternoster, Gianluca
N1 - Publisher Copyright:
© The Author(s) 2025.
PY - 2025/12/1
Y1 - 2025/12/1
N2 - Objectives: Surviving Sepsis Campaign (SSC) defined Sepsis as “life-threatening organ dysfunction caused by a dysregulated host response to infection” (De Backer D et al, Crit Care Med, n.d.). Sepsis remains one of the leading causes of morbidity and mortality (17–65% (De Oliveira DC, Arq Bras Cardiol Sociedade Brasileira de Cardiologia - SBC 94:352–6, 2010)) worldwide and it still remains a challenge to be defined and for which an appropriate treatment is desired (Chiu and Legrand, Curr Opin Anaesthesiol 34:71–6, 2021). Different studies have been conducted on genes coding for inflammatory cytokines whose could predispose to the development of sepsis [e.g., IL-10 PD1 and WT1] (Gupta DL et al, Infectious Process Sepsis, 202). Design: This multicentric observational prospective study aims to evaluate blinding the genetic expression kinetics of different molecules involved in the inflammatory process, IL10, PD1 and WT1, to search for a possible molecular predictive marker of sepsis. Setting: Nine University teaching Hospitals in Italy take part in this study in collaboration with the Department of Applied Science (DISBA) of the University of Basilicata. Participants: One hundred sixty-two patients, under elective cardiac and on pump surgery were enrolled in the study. Interventions: From each patient 4 blood samples were collected during and at the end of the surgery, following the study design. Measurements and main results: We observed, 30 min after the start of the surgery, lower gene expression levels of IL10 and PD1 in septic patients compared to non-septic (p < 0.05), but considering all the timepoint there are differences in gene expression modulation between the groups. Conclusion: These results confirmed the dysregulated immune response in septic patients compared to non-septic, highlight how a measurement of the gene expression could help to optimize procedures and pay attention to more susceptible patients.
AB - Objectives: Surviving Sepsis Campaign (SSC) defined Sepsis as “life-threatening organ dysfunction caused by a dysregulated host response to infection” (De Backer D et al, Crit Care Med, n.d.). Sepsis remains one of the leading causes of morbidity and mortality (17–65% (De Oliveira DC, Arq Bras Cardiol Sociedade Brasileira de Cardiologia - SBC 94:352–6, 2010)) worldwide and it still remains a challenge to be defined and for which an appropriate treatment is desired (Chiu and Legrand, Curr Opin Anaesthesiol 34:71–6, 2021). Different studies have been conducted on genes coding for inflammatory cytokines whose could predispose to the development of sepsis [e.g., IL-10 PD1 and WT1] (Gupta DL et al, Infectious Process Sepsis, 202). Design: This multicentric observational prospective study aims to evaluate blinding the genetic expression kinetics of different molecules involved in the inflammatory process, IL10, PD1 and WT1, to search for a possible molecular predictive marker of sepsis. Setting: Nine University teaching Hospitals in Italy take part in this study in collaboration with the Department of Applied Science (DISBA) of the University of Basilicata. Participants: One hundred sixty-two patients, under elective cardiac and on pump surgery were enrolled in the study. Interventions: From each patient 4 blood samples were collected during and at the end of the surgery, following the study design. Measurements and main results: We observed, 30 min after the start of the surgery, lower gene expression levels of IL10 and PD1 in septic patients compared to non-septic (p < 0.05), but considering all the timepoint there are differences in gene expression modulation between the groups. Conclusion: These results confirmed the dysregulated immune response in septic patients compared to non-septic, highlight how a measurement of the gene expression could help to optimize procedures and pay attention to more susceptible patients.
KW - Cardiac Surgery
KW - Cardiopulmonary bypass
KW - Gene expression
KW - Interleukins
KW - Septic shock
UR - https://www.scopus.com/pages/publications/105018469034
U2 - 10.1186/s44158-025-00277-4
DO - 10.1186/s44158-025-00277-4
M3 - Article
C2 - 41013722
SN - 2731-3786
VL - 5
JO - Journal of Anesthesia, Analgesia and Critical Care
JF - Journal of Anesthesia, Analgesia and Critical Care
IS - 1
M1 - 57
ER -