FACS-based protocol to assess cytotoxicity and clonogenic potential of colorectal cancer stem cells using a Wnt/β-catenin signaling pathway reporter

Simone di Franco; Le Zhang; Miriam Gaggianesi; Melania Lo Iacono; Jan Paul Medema; Giorgio Stassi

doi:10.1016/j.xpro.2021.100880

FACS-based protocol to assess cytotoxicity and clonogenic potential of colorectal cancer stem cells using a Wnt/β-catenin signaling pathway reporter

Simone di Franco
, Le Zhang
, Miriam Gaggianesi
, Melania Lo Iacono
, Jan Paul Medema
, Giorgio Stassi^*

^*Corresponding author for this work

University of Palermo

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Cancer stem cells (CSCs) play a key role in tumor initiation and progression. A real-time tool to evaluate the activation of CSC-specific signaling pathways is crucial for the study of this cancer cell subset. Here, we present a protocol to monitor, in vitro, the activation of Wnt/β-catenin signaling pathway, which is considered a functional biomarker for colorectal CSCs (CR-CSCs). This flow-cytometry-based protocol allows it to isolate CR-CSCs and to evaluate their cytotoxicity upon anti-tumor treatments. For complete details on the use and execution of this protocol, please refer to Di Franco et al. (2021).

Original language	English
Article number	100880
Journal	STAR Protocols
Volume	2
Issue number	4
DOIs	https://doi.org/10.1016/j.xpro.2021.100880
Publication status	Published - 17 Dec 2021

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SDG 3 Good Health and Well-being

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10.1016/j.xpro.2021.100880

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title = "FACS-based protocol to assess cytotoxicity and clonogenic potential of colorectal cancer stem cells using a Wnt/β-catenin signaling pathway reporter",

abstract = "Cancer stem cells (CSCs) play a key role in tumor initiation and progression. A real-time tool to evaluate the activation of CSC-specific signaling pathways is crucial for the study of this cancer cell subset. Here, we present a protocol to monitor, in vitro, the activation of Wnt/β-catenin signaling pathway, which is considered a functional biomarker for colorectal CSCs (CR-CSCs). This flow-cytometry-based protocol allows it to isolate CR-CSCs and to evaluate their cytotoxicity upon anti-tumor treatments. For complete details on the use and execution of this protocol, please refer to Di Franco et al. (2021).",

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year = "2021",

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doi = "10.1016/j.xpro.2021.100880",

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T1 - FACS-based protocol to assess cytotoxicity and clonogenic potential of colorectal cancer stem cells using a Wnt/β-catenin signaling pathway reporter

AU - di Franco, Simone

AU - Zhang, Le

AU - Gaggianesi, Miriam

AU - Lo Iacono, Melania

AU - Medema, Jan Paul

AU - Stassi, Giorgio

PY - 2021/12/17

Y1 - 2021/12/17

N2 - Cancer stem cells (CSCs) play a key role in tumor initiation and progression. A real-time tool to evaluate the activation of CSC-specific signaling pathways is crucial for the study of this cancer cell subset. Here, we present a protocol to monitor, in vitro, the activation of Wnt/β-catenin signaling pathway, which is considered a functional biomarker for colorectal CSCs (CR-CSCs). This flow-cytometry-based protocol allows it to isolate CR-CSCs and to evaluate their cytotoxicity upon anti-tumor treatments. For complete details on the use and execution of this protocol, please refer to Di Franco et al. (2021).

AB - Cancer stem cells (CSCs) play a key role in tumor initiation and progression. A real-time tool to evaluate the activation of CSC-specific signaling pathways is crucial for the study of this cancer cell subset. Here, we present a protocol to monitor, in vitro, the activation of Wnt/β-catenin signaling pathway, which is considered a functional biomarker for colorectal CSCs (CR-CSCs). This flow-cytometry-based protocol allows it to isolate CR-CSCs and to evaluate their cytotoxicity upon anti-tumor treatments. For complete details on the use and execution of this protocol, please refer to Di Franco et al. (2021).

UR - https://www.scopus.com/pages/publications/85122779431

UR - https://www.ncbi.nlm.nih.gov/pubmed/34712995

U2 - 10.1016/j.xpro.2021.100880

DO - 10.1016/j.xpro.2021.100880

M3 - Article

C2 - 34712995

SN - 2666-1667

VL - 2

JO - STAR Protocols

JF - STAR Protocols

IS - 4

M1 - 100880

ER -

FACS-based protocol to assess cytotoxicity and clonogenic potential of colorectal cancer stem cells using a Wnt/β-catenin signaling pathway reporter

Abstract

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