Expression of Epstein-Barr-virus-related nuclear antigens and B-cell markers in lymphomas of SIV-immunosuppressed monkeys

S L Li; H Feichtinger; E Kaaya; P Migliorini; P Putkonen; G Biberfeld; J M Middeldorp; P Biberfeld; I Ernberg

Expression of Epstein-Barr-virus-related nuclear antigens and B-cell markers in lymphomas of SIV-immunosuppressed monkeys

S L Li
, H Feichtinger
, E Kaaya
, P Migliorini
, P Putkonen
, G Biberfeld
, J M Middeldorp
, P Biberfeld
, I Ernberg

Immunopathology Laboratory, Karolinska Institute, Stockholm, Sweden.

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Simian-immunodeficiency-virus(SIV)-infected cynomolgus monkeys develop B-cell lymphomas in approximately one third of the cases. We have now studied the expression of cynomolgus-Epstein-Barr-virus(cyno-EBV) nuclear antigens in 13 cyno-EBV-carrying SIVsm-associated monkey lymphomas and established cell lines from 3 of these tumors. Immunoblots of cell lysates were probed with polyspecific and monospecific reagents directed against human EB-virus EBNAI-6, and against the membrane protein LMPI. An EBNA2-cross-reacting protein was demonstrated in 8 lymphoma tissues (8/13) and in the 3 cell lines derived from the tumors. All tumors expressed a polypeptide with 50 to 55 kDa molecular weight, which cross-reacted with some antibodies to EBNAI. Absorption experiments with normal monkey tissue showed that this polypeptide was specific for the cyno-EBV-carrying lymphoma cells. Equivalents of EBNA3-6 and LMPI could not be detected. Immunophenotypical characterization showed that the monkey lymphomas were similar to human HIV-associated B-cell lymphomas. Malignant B-cell lymphomas in experimentally SIVsm-infected cynomolgus monkeys can be a model for EBV-associated lymphomagenesis in immunodeficiency states.

Original language	English
Pages (from-to)	609-15
Number of pages	7
Journal	International Journal of Cancer
Volume	55
Issue number	4
Publication status	Published - 21 Oct 1993

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Animals
Antigens, CD
Antigens, Nuclear
Antigens, Viral
Blotting, Western
Cross Reactions
DNA-Binding Proteins
Epstein-Barr Virus Nuclear Antigens
Herpesvirus 4, Human
Immunohistochemistry
Lymphoma, B-Cell
Macaca fascicularis
Nuclear Proteins
Simian Immunodeficiency Virus
Journal Article
Research Support, Non-U.S. Gov't

Cite this

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title = "Expression of Epstein-Barr-virus-related nuclear antigens and B-cell markers in lymphomas of SIV-immunosuppressed monkeys",

abstract = "Simian-immunodeficiency-virus(SIV)-infected cynomolgus monkeys develop B-cell lymphomas in approximately one third of the cases. We have now studied the expression of cynomolgus-Epstein-Barr-virus(cyno-EBV) nuclear antigens in 13 cyno-EBV-carrying SIVsm-associated monkey lymphomas and established cell lines from 3 of these tumors. Immunoblots of cell lysates were probed with polyspecific and monospecific reagents directed against human EB-virus EBNAI-6, and against the membrane protein LMPI. An EBNA2-cross-reacting protein was demonstrated in 8 lymphoma tissues (8/13) and in the 3 cell lines derived from the tumors. All tumors expressed a polypeptide with 50 to 55 kDa molecular weight, which cross-reacted with some antibodies to EBNAI. Absorption experiments with normal monkey tissue showed that this polypeptide was specific for the cyno-EBV-carrying lymphoma cells. Equivalents of EBNA3-6 and LMPI could not be detected. Immunophenotypical characterization showed that the monkey lymphomas were similar to human HIV-associated B-cell lymphomas. Malignant B-cell lymphomas in experimentally SIVsm-infected cynomolgus monkeys can be a model for EBV-associated lymphomagenesis in immunodeficiency states.",

keywords = "Animals, Antigens, CD, Antigens, Nuclear, Antigens, Viral, Blotting, Western, Cross Reactions, DNA-Binding Proteins, Epstein-Barr Virus Nuclear Antigens, Herpesvirus 4, Human, Immunohistochemistry, Lymphoma, B-Cell, Macaca fascicularis, Nuclear Proteins, Simian Immunodeficiency Virus, Journal Article, Research Support, Non-U.S. Gov't",

author = "Li, \{S L\} and H Feichtinger and E Kaaya and P Migliorini and P Putkonen and G Biberfeld and Middeldorp, \{J M\} and P Biberfeld and I Ernberg",

year = "1993",

month = oct,

day = "21",

language = "English",

volume = "55",

pages = "609--15",

journal = "International Journal of Cancer",

issn = "0020-7136",

publisher = "Wiley-Liss Inc.",

number = "4",

}

TY - JOUR

T1 - Expression of Epstein-Barr-virus-related nuclear antigens and B-cell markers in lymphomas of SIV-immunosuppressed monkeys

AU - Li, S L

AU - Feichtinger, H

AU - Kaaya, E

AU - Migliorini, P

AU - Putkonen, P

AU - Biberfeld, G

AU - Middeldorp, J M

AU - Biberfeld, P

AU - Ernberg, I

PY - 1993/10/21

Y1 - 1993/10/21

N2 - Simian-immunodeficiency-virus(SIV)-infected cynomolgus monkeys develop B-cell lymphomas in approximately one third of the cases. We have now studied the expression of cynomolgus-Epstein-Barr-virus(cyno-EBV) nuclear antigens in 13 cyno-EBV-carrying SIVsm-associated monkey lymphomas and established cell lines from 3 of these tumors. Immunoblots of cell lysates were probed with polyspecific and monospecific reagents directed against human EB-virus EBNAI-6, and against the membrane protein LMPI. An EBNA2-cross-reacting protein was demonstrated in 8 lymphoma tissues (8/13) and in the 3 cell lines derived from the tumors. All tumors expressed a polypeptide with 50 to 55 kDa molecular weight, which cross-reacted with some antibodies to EBNAI. Absorption experiments with normal monkey tissue showed that this polypeptide was specific for the cyno-EBV-carrying lymphoma cells. Equivalents of EBNA3-6 and LMPI could not be detected. Immunophenotypical characterization showed that the monkey lymphomas were similar to human HIV-associated B-cell lymphomas. Malignant B-cell lymphomas in experimentally SIVsm-infected cynomolgus monkeys can be a model for EBV-associated lymphomagenesis in immunodeficiency states.

AB - Simian-immunodeficiency-virus(SIV)-infected cynomolgus monkeys develop B-cell lymphomas in approximately one third of the cases. We have now studied the expression of cynomolgus-Epstein-Barr-virus(cyno-EBV) nuclear antigens in 13 cyno-EBV-carrying SIVsm-associated monkey lymphomas and established cell lines from 3 of these tumors. Immunoblots of cell lysates were probed with polyspecific and monospecific reagents directed against human EB-virus EBNAI-6, and against the membrane protein LMPI. An EBNA2-cross-reacting protein was demonstrated in 8 lymphoma tissues (8/13) and in the 3 cell lines derived from the tumors. All tumors expressed a polypeptide with 50 to 55 kDa molecular weight, which cross-reacted with some antibodies to EBNAI. Absorption experiments with normal monkey tissue showed that this polypeptide was specific for the cyno-EBV-carrying lymphoma cells. Equivalents of EBNA3-6 and LMPI could not be detected. Immunophenotypical characterization showed that the monkey lymphomas were similar to human HIV-associated B-cell lymphomas. Malignant B-cell lymphomas in experimentally SIVsm-infected cynomolgus monkeys can be a model for EBV-associated lymphomagenesis in immunodeficiency states.

KW - Animals

KW - Antigens, CD

KW - Antigens, Nuclear

KW - Antigens, Viral

KW - Blotting, Western

KW - Cross Reactions

KW - DNA-Binding Proteins

KW - Epstein-Barr Virus Nuclear Antigens

KW - Herpesvirus 4, Human

KW - Immunohistochemistry

KW - Lymphoma, B-Cell

KW - Macaca fascicularis

KW - Nuclear Proteins

KW - Simian Immunodeficiency Virus

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

M3 - Article

SN - 0020-7136

VL - 55

SP - 609

EP - 615

JO - International Journal of Cancer

JF - International Journal of Cancer

IS - 4

ER -

Expression of Epstein-Barr-virus-related nuclear antigens and B-cell markers in lymphomas of SIV-immunosuppressed monkeys

Abstract

UN SDGs

Keywords

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