Estradiol reference intervals in women during the menstrual cycle, postmenopausal women and men using an LC-MS/MS method

Sara J E Verdonk; Hubert W Vesper; Frans Martens; Patrick M Sluss; Jacquelien J Hillebrand; Annemieke C Heijboer

doi:10.1016/j.cca.2019.04.062

Estradiol reference intervals in women during the menstrual cycle, postmenopausal women and men using an LC-MS/MS method

Sara J E Verdonk
, Hubert W Vesper
, Frans Martens
, Patrick M Sluss
, Jacquelien J Hillebrand
, Annemieke C Heijboer

Amsterdam UMC, University of Amsterdam, Department of Medical Informatics, Center for Human Factors Engineering of Health Information Technology (HIT-Lab), the Netherlands; Amsterdam UMC, Department of Medical Informatics, Amsterdam Public Health Research Institute, Meibergdreef 9, Amsterdam, the Netherlands.
Centers for Disease Control and Prevention
Department of Sciences, Amsterdam University College, VU Amsterdam/University of Amsterdam, 1012 WX, Amsterdam, The Netherlands
Department of Neurosurgery, Massachusetts General Hospital, Boston, Massachusetts
Amsterdam UMC, Amsterdam, Netherlands
Centers of Disease Control and Prevention, 30341-3724 Atlanta, Georgia
Clinical Pathology Core Laboratory, Boston, United States

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

BACKGROUND: For optimal medical decision-making, harmonized reference intervals for estradiol for different ages and both sexes are needed. Our aim was to establish reference intervals using a highly accurate and traceable LC-MS/MS method and to compare these with reference intervals in literature.

METHODS: Estradiol was measured in serum obtained daily during the menstrual cycle of 30 healthy premenopausal women and in serum of 64 men and 33 postmenopausal women. The accuracy of our LC-MS/MS method was demonstrated by a method comparison with the CDC reference method.

RESULTS: Our LC-MS/MS method was traceable to the reference method. Estradiol reference interval during the early follicular phase (days -15 to -6) was 31-771 pmol/L; during the late follicular phase (days -5 to -1) 104-1742 pmol/L; during the LH peak (day 0) 275-2864 pmol/L; during the early luteal phase (days +1 to +4) 95-1188 pmol/L; during mid luteal phase (days +5 to +9) 151-1941 pmol/L; during late luteal phase (days +10 to +14) 39-1769 pmol/L. The reference interval for men was 12-136 pmol/L and for postmenopausal women <26 pmol/L.

CONCLUSIONS: The established estradiol reference intervals can be used for all traceable LC-MS/MS methods for medical-decision making.

Original language	English
Pages (from-to)	198-204
Number of pages	7
Journal	Clinica chimica acta; international journal of clinical chemistry
Volume	495
Early online date	11 Apr 2019
DOIs	https://doi.org/10.1016/j.cca.2019.04.062
Publication status	Published - 1 Aug 2019

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 5 Gender Equality

Keywords

Adolescent
Adult
Blood Chemical Analysis/standards
Chromatography, Liquid
Estradiol/blood
Female
Humans
Male
Menstrual Cycle
Postmenopause/blood
Reference Values
Tandem Mass Spectrometry
Young Adult

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10.1016/j.cca.2019.04.062

2019CCA estradiol method and ref rangesFinal published version, 884 KB

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title = "Estradiol reference intervals in women during the menstrual cycle, postmenopausal women and men using an LC-MS/MS method",

abstract = "BACKGROUND: For optimal medical decision-making, harmonized reference intervals for estradiol for different ages and both sexes are needed. Our aim was to establish reference intervals using a highly accurate and traceable LC-MS/MS method and to compare these with reference intervals in literature.METHODS: Estradiol was measured in serum obtained daily during the menstrual cycle of 30 healthy premenopausal women and in serum of 64 men and 33 postmenopausal women. The accuracy of our LC-MS/MS method was demonstrated by a method comparison with the CDC reference method.RESULTS: Our LC-MS/MS method was traceable to the reference method. Estradiol reference interval during the early follicular phase (days -15 to -6) was 31-771 pmol/L; during the late follicular phase (days -5 to -1) 104-1742 pmol/L; during the LH peak (day 0) 275-2864 pmol/L; during the early luteal phase (days +1 to +4) 95-1188 pmol/L; during mid luteal phase (days +5 to +9) 151-1941 pmol/L; during late luteal phase (days +10 to +14) 39-1769 pmol/L. The reference interval for men was 12-136 pmol/L and for postmenopausal women <26 pmol/L.CONCLUSIONS: The established estradiol reference intervals can be used for all traceable LC-MS/MS methods for medical-decision making.",

keywords = "Adolescent, Adult, Blood Chemical Analysis/standards, Chromatography, Liquid, Estradiol/blood, Female, Humans, Male, Menstrual Cycle, Postmenopause/blood, Reference Values, Tandem Mass Spectrometry, Young Adult",

author = "Verdonk, \{Sara J E\} and Vesper, \{Hubert W\} and Frans Martens and Sluss, \{Patrick M\} and Hillebrand, \{Jacquelien J\} and Heijboer, \{Annemieke C\}",

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doi = "10.1016/j.cca.2019.04.062",

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volume = "495",

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journal = "Clinica chimica acta; international journal of clinical chemistry",

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T1 - Estradiol reference intervals in women during the menstrual cycle, postmenopausal women and men using an LC-MS/MS method

AU - Verdonk, Sara J E

AU - Vesper, Hubert W

AU - Martens, Frans

AU - Sluss, Patrick M

AU - Hillebrand, Jacquelien J

AU - Heijboer, Annemieke C

PY - 2019/8/1

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N2 - BACKGROUND: For optimal medical decision-making, harmonized reference intervals for estradiol for different ages and both sexes are needed. Our aim was to establish reference intervals using a highly accurate and traceable LC-MS/MS method and to compare these with reference intervals in literature.METHODS: Estradiol was measured in serum obtained daily during the menstrual cycle of 30 healthy premenopausal women and in serum of 64 men and 33 postmenopausal women. The accuracy of our LC-MS/MS method was demonstrated by a method comparison with the CDC reference method.RESULTS: Our LC-MS/MS method was traceable to the reference method. Estradiol reference interval during the early follicular phase (days -15 to -6) was 31-771 pmol/L; during the late follicular phase (days -5 to -1) 104-1742 pmol/L; during the LH peak (day 0) 275-2864 pmol/L; during the early luteal phase (days +1 to +4) 95-1188 pmol/L; during mid luteal phase (days +5 to +9) 151-1941 pmol/L; during late luteal phase (days +10 to +14) 39-1769 pmol/L. The reference interval for men was 12-136 pmol/L and for postmenopausal women <26 pmol/L.CONCLUSIONS: The established estradiol reference intervals can be used for all traceable LC-MS/MS methods for medical-decision making.

AB - BACKGROUND: For optimal medical decision-making, harmonized reference intervals for estradiol for different ages and both sexes are needed. Our aim was to establish reference intervals using a highly accurate and traceable LC-MS/MS method and to compare these with reference intervals in literature.METHODS: Estradiol was measured in serum obtained daily during the menstrual cycle of 30 healthy premenopausal women and in serum of 64 men and 33 postmenopausal women. The accuracy of our LC-MS/MS method was demonstrated by a method comparison with the CDC reference method.RESULTS: Our LC-MS/MS method was traceable to the reference method. Estradiol reference interval during the early follicular phase (days -15 to -6) was 31-771 pmol/L; during the late follicular phase (days -5 to -1) 104-1742 pmol/L; during the LH peak (day 0) 275-2864 pmol/L; during the early luteal phase (days +1 to +4) 95-1188 pmol/L; during mid luteal phase (days +5 to +9) 151-1941 pmol/L; during late luteal phase (days +10 to +14) 39-1769 pmol/L. The reference interval for men was 12-136 pmol/L and for postmenopausal women <26 pmol/L.CONCLUSIONS: The established estradiol reference intervals can be used for all traceable LC-MS/MS methods for medical-decision making.

KW - Adolescent

KW - Adult

KW - Blood Chemical Analysis/standards

KW - Chromatography, Liquid

KW - Estradiol/blood

KW - Female

KW - Humans

KW - Male

KW - Menstrual Cycle

KW - Postmenopause/blood

KW - Reference Values

KW - Tandem Mass Spectrometry

KW - Young Adult

UR - https://www.scopus.com/pages/publications/85064324168

UR - https://www.ncbi.nlm.nih.gov/pubmed/30981845

U2 - 10.1016/j.cca.2019.04.062

DO - 10.1016/j.cca.2019.04.062

M3 - Article

C2 - 30981845

SN - 0009-8981

VL - 495

SP - 198

EP - 204

JO - Clinica chimica acta; international journal of clinical chemistry

JF - Clinica chimica acta; international journal of clinical chemistry

ER -

Estradiol reference intervals in women during the menstrual cycle, postmenopausal women and men using an LC-MS/MS method

Abstract

UN SDGs

Keywords

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