Establishment of an integration-free induced pluripotent stem cell line (MUSIi005-A) from exfoliated renal epithelial cells

Bootsakorn Boonkaew; Weeradee Thummavichit; Ratchapong Netsrithong; Chinnavuth Vatanashevanopakorn; Kovit Pattanapanyasat; Methichit Wattanapanitch

doi:10.1016/j.scr.2018.05.002

Establishment of an integration-free induced pluripotent stem cell line (MUSIi005-A) from exfoliated renal epithelial cells

Bootsakorn Boonkaew
, Weeradee Thummavichit
, Ratchapong Netsrithong
, Chinnavuth Vatanashevanopakorn
, Kovit Pattanapanyasat
, Methichit Wattanapanitch^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Human induced pluripotent stem cells (iPSCs) were generated from exfoliated renal epithelial cells isolated from a urine sample of a 31-year-old healthy woman. Epithelial cells were characterized for the expression of E-cadherin and reprogrammed using non-integrating Sendai viral vectors. The urine-derived iPSC line (designated as MUSIi005-A) was karyotypically normal, expressed pluripotent markers, differentiated into cells of three embryonic germ layers, and showed no viral and transgene expressions at passage 29. Our protocol offers a non-invasive and efficient approach for iPSC generation from patients with genetic or acquired disorders.

Original language	English
Pages (from-to)	34-37
Number of pages	4
Journal	Stem cell research
Volume	30
DOIs	https://doi.org/10.1016/j.scr.2018.05.002
Publication status	Published - Jul 2018

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This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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10.1016/j.scr.2018.05.002

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@article{54e3fb8186c44abaa7cc3e7aee31c28e,

title = "Establishment of an integration-free induced pluripotent stem cell line (MUSIi005-A) from exfoliated renal epithelial cells",

abstract = "Human induced pluripotent stem cells (iPSCs) were generated from exfoliated renal epithelial cells isolated from a urine sample of a 31-year-old healthy woman. Epithelial cells were characterized for the expression of E-cadherin and reprogrammed using non-integrating Sendai viral vectors. The urine-derived iPSC line (designated as MUSIi005-A) was karyotypically normal, expressed pluripotent markers, differentiated into cells of three embryonic germ layers, and showed no viral and transgene expressions at passage 29. Our protocol offers a non-invasive and efficient approach for iPSC generation from patients with genetic or acquired disorders.",

author = "Bootsakorn Boonkaew and Weeradee Thummavichit and Ratchapong Netsrithong and Chinnavuth Vatanashevanopakorn and Kovit Pattanapanyasat and Methichit Wattanapanitch",

note = "Funding Information: This research project was supported by the Thailand Research Fund (TRF) – Distinguished Research Professor Grant, contract no. DPG5980001 and IRG 5980006 , Siriraj Research Fund, Faculty of Medicine Siriraj Hospital, Mahidol University , Grant number (IO) R016033017 . MW and CV are supported by Chalermphrakiat Grant, Faculty of Medicine Siriraj Hospital, Mahidol University. Publisher Copyright: {\textcopyright} 2018 The Authors",

year = "2018",

month = jul,

doi = "10.1016/j.scr.2018.05.002",

language = "English",

volume = "30",

pages = "34--37",

journal = "Stem cell research",

issn = "1873-5061",

publisher = "Elsevier",

}

TY - JOUR

T1 - Establishment of an integration-free induced pluripotent stem cell line (MUSIi005-A) from exfoliated renal epithelial cells

AU - Boonkaew, Bootsakorn

AU - Thummavichit, Weeradee

AU - Netsrithong, Ratchapong

AU - Vatanashevanopakorn, Chinnavuth

AU - Pattanapanyasat, Kovit

AU - Wattanapanitch, Methichit

N1 - Funding Information: This research project was supported by the Thailand Research Fund (TRF) – Distinguished Research Professor Grant, contract no. DPG5980001 and IRG 5980006 , Siriraj Research Fund, Faculty of Medicine Siriraj Hospital, Mahidol University , Grant number (IO) R016033017 . MW and CV are supported by Chalermphrakiat Grant, Faculty of Medicine Siriraj Hospital, Mahidol University. Publisher Copyright: © 2018 The Authors

PY - 2018/7

Y1 - 2018/7

N2 - Human induced pluripotent stem cells (iPSCs) were generated from exfoliated renal epithelial cells isolated from a urine sample of a 31-year-old healthy woman. Epithelial cells were characterized for the expression of E-cadherin and reprogrammed using non-integrating Sendai viral vectors. The urine-derived iPSC line (designated as MUSIi005-A) was karyotypically normal, expressed pluripotent markers, differentiated into cells of three embryonic germ layers, and showed no viral and transgene expressions at passage 29. Our protocol offers a non-invasive and efficient approach for iPSC generation from patients with genetic or acquired disorders.

AB - Human induced pluripotent stem cells (iPSCs) were generated from exfoliated renal epithelial cells isolated from a urine sample of a 31-year-old healthy woman. Epithelial cells were characterized for the expression of E-cadherin and reprogrammed using non-integrating Sendai viral vectors. The urine-derived iPSC line (designated as MUSIi005-A) was karyotypically normal, expressed pluripotent markers, differentiated into cells of three embryonic germ layers, and showed no viral and transgene expressions at passage 29. Our protocol offers a non-invasive and efficient approach for iPSC generation from patients with genetic or acquired disorders.

UR - https://www.scopus.com/pages/publications/85047008508

U2 - 10.1016/j.scr.2018.05.002

DO - 10.1016/j.scr.2018.05.002

M3 - Article

C2 - 29778975

SN - 1873-5061

VL - 30

SP - 34

EP - 37

JO - Stem cell research

JF - Stem cell research

ER -

Establishment of an integration-free induced pluripotent stem cell line (MUSIi005-A) from exfoliated renal epithelial cells

Abstract

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