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Effects of cryogenic grinding on soft tissue optical properties

  • Eric Chan
  • , Thomas Menovsky
  • , Ashley J. Welch
  • University of Texas at Austin
  • Amsterdam UMC - University of Amsterdam

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

Abstract

Optical properties of frozen-ground and intact soft tissues obtained from spectrophotometer measurements of reflectance and transmittance were compared. The tissues used in these experiments were: Calf aorta, rat jejunum, and rabbit sciatic nerve. Each tissue spedmen was frozen in liquid nitrogen and then ground with pestle and mortar into a fine powder. A tissue paste formed once the powder returned to room temperature. The tissue paste was then sandwiched between glass slides for spectrophotometric measurements. For comparison, the optical properties of the intact tissues were also measured. Total transmission and diffuse reflection were obtained on a Varian Cary 5 Spectrophotometer (400 run-850 rim). Absorption and reduced scattering coefficients of the tissues were determined with the Inverse Adding Doubling Method. Our results suggested that optical properties of soft tissue can be estimated from that of the ground tissue in the visible range.
Original languageEnglish
Title of host publicationAdvances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases II
PublisherSPIE
Pages132-143
Volume2387
DOIs
Publication statusPublished - 12 Apr 1995
Externally publishedYes
EventAdvances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases II 1995 -
Duration: 12 Apr 1995 → …

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X

Conference

ConferenceAdvances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases II 1995
Period12/04/1995 → …

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