Effect of single dose irradiation on human glioblastoma spheroids in vitro

Fabian Fehlauer; Lukas J. A. Stalpers; John Panayiotides; Patricia Kaaijk; Dionisio González González; Sieger Leenstra; Paul van der Valk; Peter Sminia

Effect of single dose irradiation on human glioblastoma spheroids in vitro

Fabian Fehlauer
, Lukas J. A. Stalpers
, John Panayiotides
, Patricia Kaaijk
, Dionisio González González
, Sieger Leenstra
, Paul van der Valk
, Peter Sminia

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Investigation of the predictive value of a radiosurgery-relevant treatment of glioblastoma spheroids. Organotypic multicellular spheroids were cultured and irradiated (20 Gy). Morphology, apoptosis and immunohistochemical expression of p53, p21, MIB-1, TGF-beta and VEGF were examined 4 h, 24 h, 7 days, and 14 days following treatment. Cell proliferation decreased, while apoptosis was increased. No morphological damage was observed. p53 expression was significantly increased after 4 h. TGF-beta and VEGF expression were only slightly altered. Particularly early changes in proliferation and apoptosis can be observed in spheroids. Individual response differences suggest spheroids of human gliomas to be useful for monitoring radiosurgery effects

Original language	English
Pages (from-to)	477-485
Journal	Oncology reports
Volume	11
Issue number	2
Publication status	Published - 2004

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This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Cite this

@article{191159bea1894656910cfa33ce99beaa,

title = "Effect of single dose irradiation on human glioblastoma spheroids in vitro",

abstract = "Investigation of the predictive value of a radiosurgery-relevant treatment of glioblastoma spheroids. Organotypic multicellular spheroids were cultured and irradiated (20 Gy). Morphology, apoptosis and immunohistochemical expression of p53, p21, MIB-1, TGF-beta and VEGF were examined 4 h, 24 h, 7 days, and 14 days following treatment. Cell proliferation decreased, while apoptosis was increased. No morphological damage was observed. p53 expression was significantly increased after 4 h. TGF-beta and VEGF expression were only slightly altered. Particularly early changes in proliferation and apoptosis can be observed in spheroids. Individual response differences suggest spheroids of human gliomas to be useful for monitoring radiosurgery effects",

author = "Fabian Fehlauer and Stalpers, \{Lukas J. A.\} and John Panayiotides and Patricia Kaaijk and \{Gonz{\'a}lez Gonz{\'a}lez\}, Dionisio and Sieger Leenstra and \{van der Valk\}, Paul and Peter Sminia",

year = "2004",

language = "English",

volume = "11",

pages = "477--485",

journal = "Oncology reports",

issn = "1021-335X",

publisher = "Spandidos Publications",

number = "2",

}

TY - JOUR

T1 - Effect of single dose irradiation on human glioblastoma spheroids in vitro

AU - Fehlauer, Fabian

AU - Stalpers, Lukas J. A.

AU - Panayiotides, John

AU - Kaaijk, Patricia

AU - González González, Dionisio

AU - Leenstra, Sieger

AU - van der Valk, Paul

AU - Sminia, Peter

PY - 2004

Y1 - 2004

N2 - Investigation of the predictive value of a radiosurgery-relevant treatment of glioblastoma spheroids. Organotypic multicellular spheroids were cultured and irradiated (20 Gy). Morphology, apoptosis and immunohistochemical expression of p53, p21, MIB-1, TGF-beta and VEGF were examined 4 h, 24 h, 7 days, and 14 days following treatment. Cell proliferation decreased, while apoptosis was increased. No morphological damage was observed. p53 expression was significantly increased after 4 h. TGF-beta and VEGF expression were only slightly altered. Particularly early changes in proliferation and apoptosis can be observed in spheroids. Individual response differences suggest spheroids of human gliomas to be useful for monitoring radiosurgery effects

AB - Investigation of the predictive value of a radiosurgery-relevant treatment of glioblastoma spheroids. Organotypic multicellular spheroids were cultured and irradiated (20 Gy). Morphology, apoptosis and immunohistochemical expression of p53, p21, MIB-1, TGF-beta and VEGF were examined 4 h, 24 h, 7 days, and 14 days following treatment. Cell proliferation decreased, while apoptosis was increased. No morphological damage was observed. p53 expression was significantly increased after 4 h. TGF-beta and VEGF expression were only slightly altered. Particularly early changes in proliferation and apoptosis can be observed in spheroids. Individual response differences suggest spheroids of human gliomas to be useful for monitoring radiosurgery effects

M3 - Article

C2 - 14719087

SN - 1021-335X

VL - 11

SP - 477

EP - 485

JO - Oncology reports

JF - Oncology reports

IS - 2

ER -

Effect of single dose irradiation on human glioblastoma spheroids in vitro

Abstract

UN SDGs

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