Diet drives quick changes in the metabolic activity and composition of human gut microbiota in a validated in vitro gut model

Marisol Aguirre; Anat Eck; Marjorie E Koenen; Paul H M Savelkoul; Andries E Budding; Koen Venema

doi:10.1016/j.resmic.2015.09.006

Diet drives quick changes in the metabolic activity and composition of human gut microbiota in a validated in vitro gut model

Marisol Aguirre
, Anat Eck
, Marjorie E Koenen
, Paul H M Savelkoul
, Andries E Budding
, Koen Venema

Top Institute of Food & Nutrition, PO Box 557, 6700 AA Wageningen, The Netherlands; Maastricht University, School for Nutrition and Translational Research in Metabolism (NUTRIM), Faculty of Health, Medicine and Life Sciences, Department of Human Biology, PO Box 616, 6200 MD Maastricht, The Netherlands; The Netherlands Organization for Applied Scientific Research...
The Netherlands Organization for Applied Scientific Research, TNO, PO Box 360, 3700 AJ Zeist, The Netherlands.
Top Institute of Food & Nutrition, PO Box 557, 6700 AA Wageningen, The Netherlands; The Netherlands Organization for Applied Scientific Research, TNO, PO Box 360, 3700 AJ Zeist, The Netherlands; Beneficial Microbes Consultancy, Johan Karschstraat 3, 6709 TN Wageningen, The Netherlands. Electronic address: [email protected].

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The aim of this study was to screen how rapidly the human gut microbiota responds to diet in an in vitro model of the proximal colon (TIM-2 system). Two experimental diets were provided to the gut bacteria: a high carbohydrate and a high protein diet. The metabolic response and the composition of the microbiota were compared to a control diet simulating an average western meal. Short-chain and branched-chain fatty acids (SCFA and BCFA, respectively) production, in addition to changes in the community composition (profiling), were measured. The activity of the microbiota reflected differences between diets, exhibiting a trade-off between saccharolytic and proteolytic fermentation when compared to the control. Diversity analysis revealed a phylum-specific response depending on the diet tested. Most changes in the microbiome composition occurred during the first 24 h of the experiment. The outcome of this study elucidates the fact that human gut bacteria quickly respond to changes in diet. In addition, it confirms that variations in the concentration of carbohydrates and proteins modify the activity and composition of the microbiota, and these changes can potentially have an impact on the health of the host.

Original language	English
Pages (from-to)	114-25
Number of pages	12
Journal	Research in microbiology
Volume	167
Issue number	2
DOIs	https://doi.org/10.1016/j.resmic.2015.09.006
Publication status	Published - 27 Oct 2015

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Biota
Colon
Diet
Fatty Acids
Fermentation
Humans
Models, Theoretical
Time Factors
Journal Article
Research Support, Non-U.S. Gov't

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10.1016/j.resmic.2015.09.006

Cite this

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title = "Diet drives quick changes in the metabolic activity and composition of human gut microbiota in a validated in vitro gut model",

abstract = "The aim of this study was to screen how rapidly the human gut microbiota responds to diet in an in vitro model of the proximal colon (TIM-2 system). Two experimental diets were provided to the gut bacteria: a high carbohydrate and a high protein diet. The metabolic response and the composition of the microbiota were compared to a control diet simulating an average western meal. Short-chain and branched-chain fatty acids (SCFA and BCFA, respectively) production, in addition to changes in the community composition (profiling), were measured. The activity of the microbiota reflected differences between diets, exhibiting a trade-off between saccharolytic and proteolytic fermentation when compared to the control. Diversity analysis revealed a phylum-specific response depending on the diet tested. Most changes in the microbiome composition occurred during the first 24 h of the experiment. The outcome of this study elucidates the fact that human gut bacteria quickly respond to changes in diet. In addition, it confirms that variations in the concentration of carbohydrates and proteins modify the activity and composition of the microbiota, and these changes can potentially have an impact on the health of the host.",

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author = "Marisol Aguirre and Anat Eck and Koenen, \{Marjorie E\} and Savelkoul, \{Paul H M\} and Budding, \{Andries E\} and Koen Venema",

year = "2015",

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doi = "10.1016/j.resmic.2015.09.006",

language = "English",

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AU - Eck, Anat

AU - Koenen, Marjorie E

AU - Savelkoul, Paul H M

AU - Budding, Andries E

AU - Venema, Koen

PY - 2015/10/27

Y1 - 2015/10/27

N2 - The aim of this study was to screen how rapidly the human gut microbiota responds to diet in an in vitro model of the proximal colon (TIM-2 system). Two experimental diets were provided to the gut bacteria: a high carbohydrate and a high protein diet. The metabolic response and the composition of the microbiota were compared to a control diet simulating an average western meal. Short-chain and branched-chain fatty acids (SCFA and BCFA, respectively) production, in addition to changes in the community composition (profiling), were measured. The activity of the microbiota reflected differences between diets, exhibiting a trade-off between saccharolytic and proteolytic fermentation when compared to the control. Diversity analysis revealed a phylum-specific response depending on the diet tested. Most changes in the microbiome composition occurred during the first 24 h of the experiment. The outcome of this study elucidates the fact that human gut bacteria quickly respond to changes in diet. In addition, it confirms that variations in the concentration of carbohydrates and proteins modify the activity and composition of the microbiota, and these changes can potentially have an impact on the health of the host.

AB - The aim of this study was to screen how rapidly the human gut microbiota responds to diet in an in vitro model of the proximal colon (TIM-2 system). Two experimental diets were provided to the gut bacteria: a high carbohydrate and a high protein diet. The metabolic response and the composition of the microbiota were compared to a control diet simulating an average western meal. Short-chain and branched-chain fatty acids (SCFA and BCFA, respectively) production, in addition to changes in the community composition (profiling), were measured. The activity of the microbiota reflected differences between diets, exhibiting a trade-off between saccharolytic and proteolytic fermentation when compared to the control. Diversity analysis revealed a phylum-specific response depending on the diet tested. Most changes in the microbiome composition occurred during the first 24 h of the experiment. The outcome of this study elucidates the fact that human gut bacteria quickly respond to changes in diet. In addition, it confirms that variations in the concentration of carbohydrates and proteins modify the activity and composition of the microbiota, and these changes can potentially have an impact on the health of the host.

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KW - Colon

KW - Diet

KW - Fatty Acids

KW - Fermentation

KW - Humans

KW - Models, Theoretical

KW - Time Factors

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

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DO - 10.1016/j.resmic.2015.09.006

M3 - Article

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JO - Research in microbiology

JF - Research in microbiology

IS - 2

ER -

Diet drives quick changes in the metabolic activity and composition of human gut microbiota in a validated in vitro gut model

Abstract

UN SDGs

Keywords

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