Dehydroepiandrosterone sulfate (DHEAS): identification of a carrier protein in human liver and brain

G. A. Kullak-Ublick; T. Fisch; M. Oswald; B. Hagenbuch; P. J. Meier; U. Beuers; G. Paumgartner

doi:10.1016/S0014-5793(98)00168-9

Dehydroepiandrosterone sulfate (DHEAS): identification of a carrier protein in human liver and brain

G. A. Kullak-Ublick
, T. Fisch
, M. Oswald
, B. Hagenbuch
, P. J. Meier
, U. Beuers
, G. Paumgartner

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Dehydroepiandrosterone sulfate (DHEAS) is the major circulating steroid in man. Pharmacologically, it exerts marked neuropsychiatric effects. Since no target receptor has been identified, we investigated whether the organic anion transporting polypeptide (OATP), a multispecific steroid carrier, transports DHEAS. Expression of the human liver OATP in Xenopus laevis oocytes resulted in high-affinity, partially Na+-dependent uptake of [3H]DHEAS (Km: 6.6 micromol/l). DHEAS transport was inhibited by bromosulfophthalein, bile acids, sulfated estrogens and dexamethasone. Northern blot analysis showed widespread expression of OATP in human brain. These data identify OATP as the first known target protein of DHEAS in human liver and brain

Original language	English
Pages (from-to)	173-176
Journal	FEBS letters
Volume	424
Issue number	3
DOIs	https://doi.org/10.1016/S0014-5793(98)00168-9
Publication status	Published - 1998

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title = "Dehydroepiandrosterone sulfate (DHEAS): identification of a carrier protein in human liver and brain",

abstract = "Dehydroepiandrosterone sulfate (DHEAS) is the major circulating steroid in man. Pharmacologically, it exerts marked neuropsychiatric effects. Since no target receptor has been identified, we investigated whether the organic anion transporting polypeptide (OATP), a multispecific steroid carrier, transports DHEAS. Expression of the human liver OATP in Xenopus laevis oocytes resulted in high-affinity, partially Na+-dependent uptake of [3H]DHEAS (Km: 6.6 micromol/l). DHEAS transport was inhibited by bromosulfophthalein, bile acids, sulfated estrogens and dexamethasone. Northern blot analysis showed widespread expression of OATP in human brain. These data identify OATP as the first known target protein of DHEAS in human liver and brain",

author = "Kullak-Ublick, \{G. A.\} and T. Fisch and M. Oswald and B. Hagenbuch and Meier, \{P. J.\} and U. Beuers and G. Paumgartner",

year = "1998",

doi = "10.1016/S0014-5793(98)00168-9",

language = "English",

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pages = "173--176",

journal = "FEBS letters",

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T1 - Dehydroepiandrosterone sulfate (DHEAS): identification of a carrier protein in human liver and brain

AU - Kullak-Ublick, G. A.

AU - Fisch, T.

AU - Oswald, M.

AU - Hagenbuch, B.

AU - Meier, P. J.

AU - Beuers, U.

AU - Paumgartner, G.

PY - 1998

Y1 - 1998

N2 - Dehydroepiandrosterone sulfate (DHEAS) is the major circulating steroid in man. Pharmacologically, it exerts marked neuropsychiatric effects. Since no target receptor has been identified, we investigated whether the organic anion transporting polypeptide (OATP), a multispecific steroid carrier, transports DHEAS. Expression of the human liver OATP in Xenopus laevis oocytes resulted in high-affinity, partially Na+-dependent uptake of [3H]DHEAS (Km: 6.6 micromol/l). DHEAS transport was inhibited by bromosulfophthalein, bile acids, sulfated estrogens and dexamethasone. Northern blot analysis showed widespread expression of OATP in human brain. These data identify OATP as the first known target protein of DHEAS in human liver and brain

AB - Dehydroepiandrosterone sulfate (DHEAS) is the major circulating steroid in man. Pharmacologically, it exerts marked neuropsychiatric effects. Since no target receptor has been identified, we investigated whether the organic anion transporting polypeptide (OATP), a multispecific steroid carrier, transports DHEAS. Expression of the human liver OATP in Xenopus laevis oocytes resulted in high-affinity, partially Na+-dependent uptake of [3H]DHEAS (Km: 6.6 micromol/l). DHEAS transport was inhibited by bromosulfophthalein, bile acids, sulfated estrogens and dexamethasone. Northern blot analysis showed widespread expression of OATP in human brain. These data identify OATP as the first known target protein of DHEAS in human liver and brain

U2 - 10.1016/S0014-5793(98)00168-9

DO - 10.1016/S0014-5793(98)00168-9

M3 - Article

C2 - 9539145

SN - 0014-5793

VL - 424

SP - 173

EP - 176

JO - FEBS letters

JF - FEBS letters

IS - 3

ER -

Dehydroepiandrosterone sulfate (DHEAS): identification of a carrier protein in human liver and brain

Abstract

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