Cysteine proteinase activity in the development of arthritis in an adjuvant model of the rat

M. H. Meijers; J. Koopdonk-Kool; S. C. Meacock; C. J. van Noorden; R. A. Bunning; M. E. Billingham

doi:10.1007/BF01972771

Cysteine proteinase activity in the development of arthritis in an adjuvant model of the rat

M. H. Meijers
, J. Koopdonk-Kool
, S. C. Meacock
, C. J. van Noorden
, R. A. Bunning
, M. E. Billingham

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Cathepsin B and L activity was studied histochemically in arthritic rat ankle joints using specific synthetic substrates in a post coupling method on unfixed and undecalcified cryostat sections of rat ankle joints. Activity was strongly increased in chondrocytes and cells of the inflamed synovium with the development of arthritis induced by the synthetic adjuvant CP20961. Activity reached a maximum 20 days after induction of arthritis and decreased as the rats entered natural remission. Cathepsin B and L were at their highest level when macrophages were present in the joint space, as shown by using monoclonal antibody markers for rat macrophages (ED1 and ED2) in a biotin-avidin immunoperoxidase assay. This suggests that the macrophage infiltrate may have stimulated proteinase production in chondrocytes through cytokine release. The profile of appearance of cysteine proteinases suggests their involvement in the breakdown of cartilage and bone in the arthritic joint

Original language	English
Pages (from-to)	C219-C221
Journal	Agents and actions
Volume	39
Issue number	Spec No
DOIs	https://doi.org/10.1007/BF01972771
Publication status	Published - 1993

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title = "Cysteine proteinase activity in the development of arthritis in an adjuvant model of the rat",

abstract = "Cathepsin B and L activity was studied histochemically in arthritic rat ankle joints using specific synthetic substrates in a post coupling method on unfixed and undecalcified cryostat sections of rat ankle joints. Activity was strongly increased in chondrocytes and cells of the inflamed synovium with the development of arthritis induced by the synthetic adjuvant CP20961. Activity reached a maximum 20 days after induction of arthritis and decreased as the rats entered natural remission. Cathepsin B and L were at their highest level when macrophages were present in the joint space, as shown by using monoclonal antibody markers for rat macrophages (ED1 and ED2) in a biotin-avidin immunoperoxidase assay. This suggests that the macrophage infiltrate may have stimulated proteinase production in chondrocytes through cytokine release. The profile of appearance of cysteine proteinases suggests their involvement in the breakdown of cartilage and bone in the arthritic joint",

author = "Meijers, \{M. H.\} and J. Koopdonk-Kool and Meacock, \{S. C.\} and \{van Noorden\}, \{C. J.\} and Bunning, \{R. A.\} and Billingham, \{M. E.\}",

year = "1993",

doi = "10.1007/BF01972771",

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TY - JOUR

T1 - Cysteine proteinase activity in the development of arthritis in an adjuvant model of the rat

AU - Meijers, M. H.

AU - Koopdonk-Kool, J.

AU - Meacock, S. C.

AU - van Noorden, C. J.

AU - Bunning, R. A.

AU - Billingham, M. E.

PY - 1993

Y1 - 1993

N2 - Cathepsin B and L activity was studied histochemically in arthritic rat ankle joints using specific synthetic substrates in a post coupling method on unfixed and undecalcified cryostat sections of rat ankle joints. Activity was strongly increased in chondrocytes and cells of the inflamed synovium with the development of arthritis induced by the synthetic adjuvant CP20961. Activity reached a maximum 20 days after induction of arthritis and decreased as the rats entered natural remission. Cathepsin B and L were at their highest level when macrophages were present in the joint space, as shown by using monoclonal antibody markers for rat macrophages (ED1 and ED2) in a biotin-avidin immunoperoxidase assay. This suggests that the macrophage infiltrate may have stimulated proteinase production in chondrocytes through cytokine release. The profile of appearance of cysteine proteinases suggests their involvement in the breakdown of cartilage and bone in the arthritic joint

AB - Cathepsin B and L activity was studied histochemically in arthritic rat ankle joints using specific synthetic substrates in a post coupling method on unfixed and undecalcified cryostat sections of rat ankle joints. Activity was strongly increased in chondrocytes and cells of the inflamed synovium with the development of arthritis induced by the synthetic adjuvant CP20961. Activity reached a maximum 20 days after induction of arthritis and decreased as the rats entered natural remission. Cathepsin B and L were at their highest level when macrophages were present in the joint space, as shown by using monoclonal antibody markers for rat macrophages (ED1 and ED2) in a biotin-avidin immunoperoxidase assay. This suggests that the macrophage infiltrate may have stimulated proteinase production in chondrocytes through cytokine release. The profile of appearance of cysteine proteinases suggests their involvement in the breakdown of cartilage and bone in the arthritic joint

U2 - 10.1007/BF01972771

DO - 10.1007/BF01972771

M3 - Article

C2 - 8273574

SN - 0065-4299

VL - 39

SP - C219-C221

JO - Agents and actions

JF - Agents and actions

IS - Spec No

ER -

Cysteine proteinase activity in the development of arthritis in an adjuvant model of the rat

Abstract

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