Cyclopentenyl cytosine and neuroblastoma SK-N-BE(2)-C cell line cells

R. J. Slingerland; A. H. van Gennip; J. M. Bodlaender; P. A. Voûte; A. B. van Kuilenburg

doi:10.1016/0959-8049(95)00071-P

Cyclopentenyl cytosine and neuroblastoma SK-N-BE(2)-C cell line cells

R. J. Slingerland
, A. H. van Gennip
, J. M. Bodlaender
, P. A. Voûte
, A. B. van Kuilenburg

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

We studied the effect of cyclopentenyl cytosine (CPEC) on human neuroblastoma SK-N-BE(2)-C cell line cells. CPEC had an IC50 value of 100 nM for non-synchronised SK-N-BE(2)-C cells. These cells were arrested in G0/G1-phase or early S-phase of the cell cycle upon treatment with CPEC. After treatment of synchronised S-phase cells with 1 microM CPEC, the number of cells present after 3 days was less than 10% of that observed for the untreated cells. S-phase synchronised cells treated with CPEC and deoxycytidine showed an increased viability in comparison with cells treated with CPEC alone. Approximately 15% of the cells treated with CPEC and deoxycytidine traversed through one cell cycle. The amount of CTP declined to undetectable levels within 3 h after addition of 1 microM CPEC. The presence of cytidine prevented, to a large extent, the cytostatic effect of CPEC

Original language	English
Pages (from-to)	627-631
Journal	European journal of cancer (Oxford, England
Volume	31A
Issue number	4
DOIs	https://doi.org/10.1016/0959-8049(95)00071-P
Publication status	Published - 1995

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10.1016/0959-8049(95)00071-P

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@article{c2295efd12fa4147a19675230d35e930,

title = "Cyclopentenyl cytosine and neuroblastoma SK-N-BE(2)-C cell line cells",

abstract = "We studied the effect of cyclopentenyl cytosine (CPEC) on human neuroblastoma SK-N-BE(2)-C cell line cells. CPEC had an IC50 value of 100 nM for non-synchronised SK-N-BE(2)-C cells. These cells were arrested in G0/G1-phase or early S-phase of the cell cycle upon treatment with CPEC. After treatment of synchronised S-phase cells with 1 microM CPEC, the number of cells present after 3 days was less than 10\% of that observed for the untreated cells. S-phase synchronised cells treated with CPEC and deoxycytidine showed an increased viability in comparison with cells treated with CPEC alone. Approximately 15\% of the cells treated with CPEC and deoxycytidine traversed through one cell cycle. The amount of CTP declined to undetectable levels within 3 h after addition of 1 microM CPEC. The presence of cytidine prevented, to a large extent, the cytostatic effect of CPEC",

author = "Slingerland, \{R. J.\} and \{van Gennip\}, \{A. H.\} and Bodlaender, \{J. M.\} and Vo{\^u}te, \{P. A.\} and \{van Kuilenburg\}, \{A. B.\}",

year = "1995",

doi = "10.1016/0959-8049(95)00071-P",

language = "English",

volume = "31A",

pages = "627--631",

journal = "European journal of cancer (Oxford, England",

issn = "0959-8049",

publisher = "Elsevier Limited",

number = "4",

}

TY - JOUR

T1 - Cyclopentenyl cytosine and neuroblastoma SK-N-BE(2)-C cell line cells

AU - Slingerland, R. J.

AU - van Gennip, A. H.

AU - Bodlaender, J. M.

AU - Voûte, P. A.

AU - van Kuilenburg, A. B.

PY - 1995

Y1 - 1995

N2 - We studied the effect of cyclopentenyl cytosine (CPEC) on human neuroblastoma SK-N-BE(2)-C cell line cells. CPEC had an IC50 value of 100 nM for non-synchronised SK-N-BE(2)-C cells. These cells were arrested in G0/G1-phase or early S-phase of the cell cycle upon treatment with CPEC. After treatment of synchronised S-phase cells with 1 microM CPEC, the number of cells present after 3 days was less than 10% of that observed for the untreated cells. S-phase synchronised cells treated with CPEC and deoxycytidine showed an increased viability in comparison with cells treated with CPEC alone. Approximately 15% of the cells treated with CPEC and deoxycytidine traversed through one cell cycle. The amount of CTP declined to undetectable levels within 3 h after addition of 1 microM CPEC. The presence of cytidine prevented, to a large extent, the cytostatic effect of CPEC

AB - We studied the effect of cyclopentenyl cytosine (CPEC) on human neuroblastoma SK-N-BE(2)-C cell line cells. CPEC had an IC50 value of 100 nM for non-synchronised SK-N-BE(2)-C cells. These cells were arrested in G0/G1-phase or early S-phase of the cell cycle upon treatment with CPEC. After treatment of synchronised S-phase cells with 1 microM CPEC, the number of cells present after 3 days was less than 10% of that observed for the untreated cells. S-phase synchronised cells treated with CPEC and deoxycytidine showed an increased viability in comparison with cells treated with CPEC alone. Approximately 15% of the cells treated with CPEC and deoxycytidine traversed through one cell cycle. The amount of CTP declined to undetectable levels within 3 h after addition of 1 microM CPEC. The presence of cytidine prevented, to a large extent, the cytostatic effect of CPEC

U2 - 10.1016/0959-8049(95)00071-P

DO - 10.1016/0959-8049(95)00071-P

M3 - Article

C2 - 7576983

SN - 0959-8049

VL - 31A

SP - 627

EP - 631

JO - European journal of cancer (Oxford, England

JF - European journal of cancer (Oxford, England

IS - 4

ER -

Cyclopentenyl cytosine and neuroblastoma SK-N-BE(2)-C cell line cells

Abstract

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