Comparison of three different PCR methods for quantifying human papillomavirus type 16 DNA in cervical scrape specimens

A. T. Hesselink; A. J.C. Van Den Brule; Z. M.A. Groothuismink; M. Molano; J. Berkhof; C. J.L.M. Meijer; P. J.F. Snijders

doi:10.1128/JCM.43.9.4868-4871.2005

Comparison of three different PCR methods for quantifying human papillomavirus type 16 DNA in cervical scrape specimens

A. T. Hesselink
, A. J.C. Van Den Brule
, Z. M.A. Groothuismink
, M. Molano
, J. Berkhof
, C. J.L.M. Meijer
, P. J.F. Snijders^*

^*Corresponding author for this work

VU Medisch Centrum

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

We compared real-time LightCycler and TaqMan assays and the GP5+/6+ PCR/enzyme immunoassay (EIA) to assess the human papillomavirus type 16 (HPV16) load in cervical scrape specimens. Both real-time PCR assays determined the HPV16 load in scrape specimens similarly. The level of agreement between these assays and the GP5+/6+ PCR/EIA was low (P = 0.004), suggesting that the latter method is not suited for quantifying HPV16 DNA.

Original language	English
Pages (from-to)	4868-4871
Number of pages	4
Journal	Journal of clinical microbiology
Volume	43
Issue number	9
DOIs	https://doi.org/10.1128/JCM.43.9.4868-4871.2005
Publication status	Published - 1 Sept 2005

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title = "Comparison of three different PCR methods for quantifying human papillomavirus type 16 DNA in cervical scrape specimens",

abstract = "We compared real-time LightCycler and TaqMan assays and the GP5+/6+ PCR/enzyme immunoassay (EIA) to assess the human papillomavirus type 16 (HPV16) load in cervical scrape specimens. Both real-time PCR assays determined the HPV16 load in scrape specimens similarly. The level of agreement between these assays and the GP5+/6+ PCR/EIA was low (P = 0.004), suggesting that the latter method is not suited for quantifying HPV16 DNA.",

author = "Hesselink, \{A. T.\} and \{Van Den Brule\}, \{A. J.C.\} and Groothuismink, \{Z. M.A.\} and M. Molano and J. Berkhof and Meijer, \{C. J.L.M.\} and Snijders, \{P. J.F.\}",

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AU - Hesselink, A. T.

AU - Van Den Brule, A. J.C.

AU - Groothuismink, Z. M.A.

AU - Molano, M.

AU - Berkhof, J.

AU - Meijer, C. J.L.M.

AU - Snijders, P. J.F.

PY - 2005/9/1

Y1 - 2005/9/1

N2 - We compared real-time LightCycler and TaqMan assays and the GP5+/6+ PCR/enzyme immunoassay (EIA) to assess the human papillomavirus type 16 (HPV16) load in cervical scrape specimens. Both real-time PCR assays determined the HPV16 load in scrape specimens similarly. The level of agreement between these assays and the GP5+/6+ PCR/EIA was low (P = 0.004), suggesting that the latter method is not suited for quantifying HPV16 DNA.

AB - We compared real-time LightCycler and TaqMan assays and the GP5+/6+ PCR/enzyme immunoassay (EIA) to assess the human papillomavirus type 16 (HPV16) load in cervical scrape specimens. Both real-time PCR assays determined the HPV16 load in scrape specimens similarly. The level of agreement between these assays and the GP5+/6+ PCR/EIA was low (P = 0.004), suggesting that the latter method is not suited for quantifying HPV16 DNA.

UR - https://www.scopus.com/pages/publications/24744462312

U2 - 10.1128/JCM.43.9.4868-4871.2005

DO - 10.1128/JCM.43.9.4868-4871.2005

M3 - Article

C2 - 16145162

SN - 0095-1137

VL - 43

SP - 4868

EP - 4871

JO - Journal of clinical microbiology

JF - Journal of clinical microbiology

IS - 9

ER -

Comparison of three different PCR methods for quantifying human papillomavirus type 16 DNA in cervical scrape specimens

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