Characterization of the Leptospira interrogans S10-spc-α operon

R. L. Zuerner; R. A. Hartskeerl; H. van de Kemp; A. E. Bal

doi:10.1016/S0378-1097(99)00598-4

Characterization of the Leptospira interrogans S10-spc-α operon

R. L. Zuerner^*
, R. A. Hartskeerl
, H. van de Kemp
, A. E. Bal

^*Corresponding author for this work

United States Department of Agriculture
WHO/FAO/OIE and National Collaborating Centre for Reference and Research on Leptospirosis

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

A ribosomal protein gene cluster from the spirochaete Leptospira interrogans was characterized. This locus is homologous to the Escherichia coli S10, spc, and α operons. Analysis of L. interrogans RNA showed that the ribosomal protein genes within this cluster are co-transcribed, thus forming an operon. Two transcription initiation sites were mapped by primer extension, upstream of fus, the first gene in this cluster, and sequences from this region provided promoter activity in E. coli. Transcription terminates near a predicted stem-loop structure following rplQ, the last gene in the cluster. These data suggest that two promoters upstream of fus direct transcription of this 17.5-kb ribosomal protein gene cluster. Comparison of the L. interrogans S10-spc-α cluster to homologous loci from Borrelia burgdorferi and Treponema pallidum provided evidence that this region of the genome underwent several rearrangements during spirochaete evolution. Copyright (C) 2000.

Original language	English
Pages (from-to)	303-308
Journal	FEMS microbiology letters
Volume	182
Issue number	2
DOIs	https://doi.org/10.1016/S0378-1097(99)00598-4
Publication status	Published - 15 Jan 2000
Externally published	Yes

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title = "Characterization of the Leptospira interrogans S10-spc-α operon",

abstract = "A ribosomal protein gene cluster from the spirochaete Leptospira interrogans was characterized. This locus is homologous to the Escherichia coli S10, spc, and α operons. Analysis of L. interrogans RNA showed that the ribosomal protein genes within this cluster are co-transcribed, thus forming an operon. Two transcription initiation sites were mapped by primer extension, upstream of fus, the first gene in this cluster, and sequences from this region provided promoter activity in E. coli. Transcription terminates near a predicted stem-loop structure following rplQ, the last gene in the cluster. These data suggest that two promoters upstream of fus direct transcription of this 17.5-kb ribosomal protein gene cluster. Comparison of the L. interrogans S10-spc-α cluster to homologous loci from Borrelia burgdorferi and Treponema pallidum provided evidence that this region of the genome underwent several rearrangements during spirochaete evolution. Copyright (C) 2000.",

author = "Zuerner, \{R. L.\} and Hartskeerl, \{R. A.\} and \{van de Kemp\}, H. and Bal, \{A. E.\}",

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doi = "10.1016/S0378-1097(99)00598-4",

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T1 - Characterization of the Leptospira interrogans S10-spc-α operon

AU - Zuerner, R. L.

AU - Hartskeerl, R. A.

AU - van de Kemp, H.

AU - Bal, A. E.

PY - 2000/1/15

Y1 - 2000/1/15

N2 - A ribosomal protein gene cluster from the spirochaete Leptospira interrogans was characterized. This locus is homologous to the Escherichia coli S10, spc, and α operons. Analysis of L. interrogans RNA showed that the ribosomal protein genes within this cluster are co-transcribed, thus forming an operon. Two transcription initiation sites were mapped by primer extension, upstream of fus, the first gene in this cluster, and sequences from this region provided promoter activity in E. coli. Transcription terminates near a predicted stem-loop structure following rplQ, the last gene in the cluster. These data suggest that two promoters upstream of fus direct transcription of this 17.5-kb ribosomal protein gene cluster. Comparison of the L. interrogans S10-spc-α cluster to homologous loci from Borrelia burgdorferi and Treponema pallidum provided evidence that this region of the genome underwent several rearrangements during spirochaete evolution. Copyright (C) 2000.

AB - A ribosomal protein gene cluster from the spirochaete Leptospira interrogans was characterized. This locus is homologous to the Escherichia coli S10, spc, and α operons. Analysis of L. interrogans RNA showed that the ribosomal protein genes within this cluster are co-transcribed, thus forming an operon. Two transcription initiation sites were mapped by primer extension, upstream of fus, the first gene in this cluster, and sequences from this region provided promoter activity in E. coli. Transcription terminates near a predicted stem-loop structure following rplQ, the last gene in the cluster. These data suggest that two promoters upstream of fus direct transcription of this 17.5-kb ribosomal protein gene cluster. Comparison of the L. interrogans S10-spc-α cluster to homologous loci from Borrelia burgdorferi and Treponema pallidum provided evidence that this region of the genome underwent several rearrangements during spirochaete evolution. Copyright (C) 2000.

UR - https://www.scopus.com/pages/publications/0033988197

UR - https://www.ncbi.nlm.nih.gov/pubmed/10620683

U2 - 10.1016/S0378-1097(99)00598-4

DO - 10.1016/S0378-1097(99)00598-4

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VL - 182

SP - 303

EP - 308

JO - FEMS microbiology letters

JF - FEMS microbiology letters

IS - 2

ER -

Characterization of the Leptospira interrogans S10-spc-α operon

Abstract

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