Belimumab concentration measurements using a homologous anti-idiotype immunoassay

Floris C. Loeff; Ioannis Parodis; Tomas Walhelm; Andreas Jönsen; Dionysis Nikolopoulos; Christopher Sjöwall; Anders A. Bengtsson; Dorien Kos; Astrid van Leeuwen; Bryan van den Broek; Lisanne Dijk; Jorn Jeremiasse; Birgit S. Blomjous; Alexandre E. Voskuyl; Gerrit Jan Wolbink; Irene E. M. Bultink; Theo Rispens

doi:10.1016/j.jim.2024.113717

Belimumab concentration measurements using a homologous anti-idiotype immunoassay

Floris C. Loeff
, Ioannis Parodis
, Tomas Walhelm
, Andreas Jönsen
, Dionysis Nikolopoulos
, Christopher Sjöwall
, Anders A. Bengtsson
, Dorien Kos
, Astrid van Leeuwen
, Bryan van den Broek
, Lisanne Dijk
, Jorn Jeremiasse
, Birgit S. Blomjous
, Alexandre E. Voskuyl
, Gerrit Jan Wolbink
, Irene E. M. Bultink
, Theo Rispens^*

^*Corresponding author for this work

Sanquin Blood Supply Foundation
Karolinska Institutet
Örebro University
Linköping University
Lund University
Amsterdam UMC - University of Amsterdam
Amsterdam UMC
Amsterdam Rheumatology and Immunology Center and Reade, Amsterdam, The Netherlands
Karolinska University Hospital
Amsterdam University Medical Centers
Amsterdam Institute for Infection and Immunity
Amsterdam Rheumatology & Immunology Center
University of Amsterdam

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Monitoring belimumab concentrations in patients can be a valuable tool for assessing treatment response and for personalizing drug doses. Various assay formats may be used to measure concentrations of therapeutic monoclonal antibodies. A particularly useful format involves the use of anti-idiotype monoclonal antibodies, selected to be highly specific to the antibody of interest. Here, we describe the development of a specific, high-affinity anti-idiotype antibody to belimumab, and the application of this antibody in a homologous sandwich ELISA to measure belimumab concentrations.

Original language	English
Article number	113717
Journal	Journal of immunological methods
Volume	532
DOIs	https://doi.org/10.1016/j.jim.2024.113717
Publication status	Published - 1 Sept 2024

Keywords

Anti-idiotype antibodies
Belimumab
PK/ADA assays
Rabbit monoclonal antibodies

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Belimumab-concentration-measurements-using-a-homologous-anti-idiotype-immunoassay.pdfFinal published version, 405 KBLicence: Taverne

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Loeff, F. C., Parodis, I., Walhelm, T., Jönsen, A., Nikolopoulos, D., Sjöwall, C., Bengtsson, A. A., Kos, D., van Leeuwen, A., van den Broek, B., Dijk, L., Jeremiasse, J., Blomjous, B. S., Voskuyl, A. E., Wolbink, G. J., Bultink, I. E. M., & Rispens, T. (2024). Belimumab concentration measurements using a homologous anti-idiotype immunoassay. Journal of immunological methods, 532, Article 113717. https://doi.org/10.1016/j.jim.2024.113717

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title = "Belimumab concentration measurements using a homologous anti-idiotype immunoassay",

abstract = "Monitoring belimumab concentrations in patients can be a valuable tool for assessing treatment response and for personalizing drug doses. Various assay formats may be used to measure concentrations of therapeutic monoclonal antibodies. A particularly useful format involves the use of anti-idiotype monoclonal antibodies, selected to be highly specific to the antibody of interest. Here, we describe the development of a specific, high-affinity anti-idiotype antibody to belimumab, and the application of this antibody in a homologous sandwich ELISA to measure belimumab concentrations.",

keywords = "Anti-idiotype antibodies, Belimumab, PK/ADA assays, Rabbit monoclonal antibodies",

author = "Loeff, \{Floris C.\} and Ioannis Parodis and Tomas Walhelm and Andreas J{\"o}nsen and Dionysis Nikolopoulos and Christopher Sj{\"o}wall and Bengtsson, \{Anders A.\} and Dorien Kos and \{van Leeuwen\}, Astrid and \{van den Broek\}, Bryan and Lisanne Dijk and Jorn Jeremiasse and Blomjous, \{Birgit S.\} and Voskuyl, \{Alexandre E.\} and Wolbink, \{Gerrit Jan\} and Bultink, \{Irene E. M.\} and Theo Rispens",

note = "Publisher Copyright: {\textcopyright} 2024 Elsevier B.V.",

year = "2024",

month = sep,

day = "1",

doi = "10.1016/j.jim.2024.113717",

language = "English",

volume = "532",

journal = "Journal of immunological methods",

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Loeff, FC, Parodis, I, Walhelm, T, Jönsen, A, Nikolopoulos, D, Sjöwall, C, Bengtsson, AA, Kos, D, van Leeuwen, A, van den Broek, B, Dijk, L, Jeremiasse, J, Blomjous, BS , Voskuyl, AE , Wolbink, GJ , Bultink, IEM & Rispens, T 2024, 'Belimumab concentration measurements using a homologous anti-idiotype immunoassay', Journal of immunological methods, vol. 532, 113717. https://doi.org/10.1016/j.jim.2024.113717

TY - JOUR

T1 - Belimumab concentration measurements using a homologous anti-idiotype immunoassay

AU - Loeff, Floris C.

AU - Parodis, Ioannis

AU - Walhelm, Tomas

AU - Jönsen, Andreas

AU - Nikolopoulos, Dionysis

AU - Sjöwall, Christopher

AU - Bengtsson, Anders A.

AU - Kos, Dorien

AU - van Leeuwen, Astrid

AU - van den Broek, Bryan

AU - Dijk, Lisanne

AU - Jeremiasse, Jorn

AU - Blomjous, Birgit S.

AU - Voskuyl, Alexandre E.

AU - Wolbink, Gerrit Jan

AU - Bultink, Irene E. M.

AU - Rispens, Theo

PY - 2024/9/1

Y1 - 2024/9/1

N2 - Monitoring belimumab concentrations in patients can be a valuable tool for assessing treatment response and for personalizing drug doses. Various assay formats may be used to measure concentrations of therapeutic monoclonal antibodies. A particularly useful format involves the use of anti-idiotype monoclonal antibodies, selected to be highly specific to the antibody of interest. Here, we describe the development of a specific, high-affinity anti-idiotype antibody to belimumab, and the application of this antibody in a homologous sandwich ELISA to measure belimumab concentrations.

AB - Monitoring belimumab concentrations in patients can be a valuable tool for assessing treatment response and for personalizing drug doses. Various assay formats may be used to measure concentrations of therapeutic monoclonal antibodies. A particularly useful format involves the use of anti-idiotype monoclonal antibodies, selected to be highly specific to the antibody of interest. Here, we describe the development of a specific, high-affinity anti-idiotype antibody to belimumab, and the application of this antibody in a homologous sandwich ELISA to measure belimumab concentrations.

KW - Anti-idiotype antibodies

KW - Belimumab

KW - PK/ADA assays

KW - Rabbit monoclonal antibodies

UR - https://www.scopus.com/pages/publications/85197474221

U2 - 10.1016/j.jim.2024.113717

DO - 10.1016/j.jim.2024.113717

M3 - Article

C2 - 38960066

SN - 0022-1759

VL - 532

JO - Journal of immunological methods

JF - Journal of immunological methods

M1 - 113717

ER -

Belimumab concentration measurements using a homologous anti-idiotype immunoassay

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