TY - JOUR
T1 - Balancing Scaffold Degradation and Neo-Tissue Formation in In Situ Tissue Engineered Vascular Grafts
AU - Uiterwijk, Marcelle
AU - Coolen, Bram F.
AU - van Rijswijk, Jan-Willem
AU - Söntjens, Serge H. M.
AU - van Houtem, Michel H. C. J.
AU - Szymczyk, Wojciech
AU - Rijns, Laura
AU - Janssen, Henk M.
AU - van de Wal, Allard
AU - de Mol, Bas A. J. M.
AU - Bouten, Carlijn V. C.
AU - Strijkers, Gustav J.
AU - Dankers, Patricia Y. W.
AU - Kluin, Jolanda
N1 - Publisher Copyright:
© Mary Ann Liebert, Inc.
PY - 2024/8/1
Y1 - 2024/8/1
N2 - An essential aspect of cardiovascular in situ tissue engineering (TE) is to ensure balance between scaffold degradation and neo-tissue formation. We evaluated the rate of degradation and neo-tissue formation of three electrospun supramolecular bisurea-based biodegradable scaffolds that differ in their soft-block backbone compositions only. Scaffolds were implanted as interposition grafts in the abdominal aorta in rats, and evaluated at different time points (t = 1, 6, 12, 24, and 40 weeks) on function, tissue formation, strength, and scaffold degradation. The fully carbonate-based biomaterial showed minor degradation after 40 weeks in vivo, whereas the other two ester-containing biomaterials showed (near) complete degradation within 6-12 weeks. Local dilatation was only observed in these faster degrading scaffolds. All materials showed to some extent mineralization, at early as well as late time points. Histological evaluation showed equal and non-native-like neo-tissue formation after total degradation. The fully carbonate-based scaffolds lagged in neo-tissue formation, presumably as its degradation was (far from) complete at 40 weeks. A significant difference in vessel wall contrast enhancement was observed by magnetic resonance imaging between grafts with total compared with minimal-degraded scaffolds.
AB - An essential aspect of cardiovascular in situ tissue engineering (TE) is to ensure balance between scaffold degradation and neo-tissue formation. We evaluated the rate of degradation and neo-tissue formation of three electrospun supramolecular bisurea-based biodegradable scaffolds that differ in their soft-block backbone compositions only. Scaffolds were implanted as interposition grafts in the abdominal aorta in rats, and evaluated at different time points (t = 1, 6, 12, 24, and 40 weeks) on function, tissue formation, strength, and scaffold degradation. The fully carbonate-based biomaterial showed minor degradation after 40 weeks in vivo, whereas the other two ester-containing biomaterials showed (near) complete degradation within 6-12 weeks. Local dilatation was only observed in these faster degrading scaffolds. All materials showed to some extent mineralization, at early as well as late time points. Histological evaluation showed equal and non-native-like neo-tissue formation after total degradation. The fully carbonate-based scaffolds lagged in neo-tissue formation, presumably as its degradation was (far from) complete at 40 weeks. A significant difference in vessel wall contrast enhancement was observed by magnetic resonance imaging between grafts with total compared with minimal-degraded scaffolds.
KW - MRI
KW - in situ tissue engineering
KW - scaffold degradation
KW - sequence-controlled biomaterials
KW - small vascular grafts
UR - https://www.scopus.com/pages/publications/85189137084
U2 - 10.1089/ten.tea.2023.0019
DO - 10.1089/ten.tea.2023.0019
M3 - Article
C2 - 38420632
SN - 1937-3341
VL - 30
SP - 421
EP - 436
JO - Tissue Engineering - Part A
JF - Tissue Engineering - Part A
IS - 15-16
ER -