Applying CRISPR-Cas9 Genome Editing to Study Genes Involved in Peroxisome Biogenesis or Peroxisomal Functions

Serhii Chornyi; Janet Koster; Hans R. Waterham

doi:10.1007/978-1-0716-3048-8_17

Applying CRISPR-Cas9 Genome Editing to Study Genes Involved in Peroxisome Biogenesis or Peroxisomal Functions

Serhii Chornyi
, Janet Koster
, Hans R. Waterham

Amsterdam UMC - University of Amsterdam
Amsterdam University Medical Centers

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

The development and application of the CRISPR-Cas9 technology for genome editing of mammalian cells have opened up a wealth of possibilities for genetically modifying and manipulating human cells, and use in functional studies or therapeutic approaches. Here we describe the approach that we have been using successfully to generate multiple human cell lines with targeted (partial) gene deletions, i.e., knockout cells, or human cells with modified genomic nucleotide sequences, i.e., knock-in cells, in genes encoding known or putative proteins involved in peroxisome biogenesis or peroxisomal functions.

Original language	English
Pages (from-to)	233-245
Number of pages	13
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	2643
DOIs	https://doi.org/10.1007/978-1-0716-3048-8_17
Publication status	Published - 2023

Keywords

CRISPR-Cas9
Gene deletion
Gene disruption
Gene editing
Gene knockout
PEX genes

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10.1007/978-1-0716-3048-8_17

Peroxisomes 2nd edition Springer Chapter 17Accepted author manuscript, 698 KB

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abstract = "The development and application of the CRISPR-Cas9 technology for genome editing of mammalian cells have opened up a wealth of possibilities for genetically modifying and manipulating human cells, and use in functional studies or therapeutic approaches. Here we describe the approach that we have been using successfully to generate multiple human cell lines with targeted (partial) gene deletions, i.e., knockout cells, or human cells with modified genomic nucleotide sequences, i.e., knock-in cells, in genes encoding known or putative proteins involved in peroxisome biogenesis or peroxisomal functions.",

keywords = "CRISPR-Cas9, Gene deletion, Gene disruption, Gene editing, Gene knockout, PEX genes",

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AU - Waterham, Hans R.

N1 - Funding Information: This project has received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement No 812968 (PERICO). Publisher Copyright: © 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

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AB - The development and application of the CRISPR-Cas9 technology for genome editing of mammalian cells have opened up a wealth of possibilities for genetically modifying and manipulating human cells, and use in functional studies or therapeutic approaches. Here we describe the approach that we have been using successfully to generate multiple human cell lines with targeted (partial) gene deletions, i.e., knockout cells, or human cells with modified genomic nucleotide sequences, i.e., knock-in cells, in genes encoding known or putative proteins involved in peroxisome biogenesis or peroxisomal functions.

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KW - Gene knockout

KW - PEX genes

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Applying CRISPR-Cas9 Genome Editing to Study Genes Involved in Peroxisome Biogenesis or Peroxisomal Functions

Abstract

Keywords

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