Apical gene transfer into quiescent human and canine polarized intestinal epithelial cells by lentivirus vectors

J. Seppen; S. C. Barry; J. H. Klinkspoor; L. J. Katen; S. P. Lee; J. V. Garcia; W. R. Osborne

doi:10.1128/JVI.74.16.7642-7645.2000

Apical gene transfer into quiescent human and canine polarized intestinal epithelial cells by lentivirus vectors

J. Seppen
, S. C. Barry
, J. H. Klinkspoor
, L. J. Katen
, S. P. Lee
, J. V. Garcia
, W. R. Osborne

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Intestinal epithelial cells secrete a protective luminal mucus barrier inhibiting viral gene transfer. Quiescent, polarized monolayers of primary epithelial cells from dog gallbladder and human colon are efficiently transduced through the apical mucus side by lentivirus vectors, suggesting their application to intestinal gene therapy

Original language	English
Pages (from-to)	7642-7645
Journal	Journal of virology
Volume	74
Issue number	16
DOIs	https://doi.org/10.1128/JVI.74.16.7642-7645.2000
Publication status	Published - 2000

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title = "Apical gene transfer into quiescent human and canine polarized intestinal epithelial cells by lentivirus vectors",

abstract = "Intestinal epithelial cells secrete a protective luminal mucus barrier inhibiting viral gene transfer. Quiescent, polarized monolayers of primary epithelial cells from dog gallbladder and human colon are efficiently transduced through the apical mucus side by lentivirus vectors, suggesting their application to intestinal gene therapy",

author = "J. Seppen and Barry, \{S. C.\} and Klinkspoor, \{J. H.\} and Katen, \{L. J.\} and Lee, \{S. P.\} and Garcia, \{J. V.\} and Osborne, \{W. R.\}",

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AU - Seppen, J.

AU - Barry, S. C.

AU - Klinkspoor, J. H.

AU - Katen, L. J.

AU - Lee, S. P.

AU - Garcia, J. V.

AU - Osborne, W. R.

PY - 2000

Y1 - 2000

N2 - Intestinal epithelial cells secrete a protective luminal mucus barrier inhibiting viral gene transfer. Quiescent, polarized monolayers of primary epithelial cells from dog gallbladder and human colon are efficiently transduced through the apical mucus side by lentivirus vectors, suggesting their application to intestinal gene therapy

AB - Intestinal epithelial cells secrete a protective luminal mucus barrier inhibiting viral gene transfer. Quiescent, polarized monolayers of primary epithelial cells from dog gallbladder and human colon are efficiently transduced through the apical mucus side by lentivirus vectors, suggesting their application to intestinal gene therapy

U2 - 10.1128/JVI.74.16.7642-7645.2000

DO - 10.1128/JVI.74.16.7642-7645.2000

M3 - Article

C2 - 10906219

SN - 0022-538X

VL - 74

SP - 7642

EP - 7645

JO - Journal of virology

JF - Journal of virology

IS - 16

ER -

Apical gene transfer into quiescent human and canine polarized intestinal epithelial cells by lentivirus vectors

Abstract

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