Altered Loyalties of Neuronal Markers in Cultured Slices of Resected Human Brain Tissue

Ronald W. H. Verwer; Arja A. Sluiter; Rawien A. Balesar; Johannes C. Baayen; Dave Speijer; Sander Idema; Dick F. Swaab

doi:10.1111/bpa.12330

Altered Loyalties of Neuronal Markers in Cultured Slices of Resected Human Brain Tissue

Ronald W. H. Verwer
, Arja A. Sluiter
, Rawien A. Balesar
, Johannes C. Baayen
, Dave Speijer
, Sander Idema
, Dick F. Swaab

Netherlands Institute for Neuroscience
University of Amsterdam

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Organotypic cultures from normal neocortical tissue obtained at epilepsy surgery show a severe injury response. This response involves both neuronal degeneration and the proliferation of reactive cells. A salient feature of the reactive cells is the co-expression of microglial and astrocytic markers. Surprisingly, the reactive cells also began to express neuronal markers Tubulin βIII and MAP2 adding to the confusion about their origin. Concomitant with their appearance in reactive cells MAP2 and Tubulin βIII expression disappeared from neurons. While NeuN expression decreased significantly, it did not entirely disappear from many neurons. Moreover, it was not observed in reactive cells, showing that NeuN is a reliable marker of neurons

Original language	English
Pages (from-to)	523-532
Number of pages	10
Journal	Brain pathology (Zurich, Switzerland)
Volume	26
Issue number	4
DOIs	https://doi.org/10.1111/bpa.12330
Publication status	Published - 1 Jul 2016

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This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

brain injury
epilepsy
human neocortex
neuronal degeneration
neuronal markers
reactive glial cells
tissue culture

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10.1111/bpa.12330

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Cite this

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title = "Altered Loyalties of Neuronal Markers in Cultured Slices of Resected Human Brain Tissue",

abstract = "Organotypic cultures from normal neocortical tissue obtained at epilepsy surgery show a severe injury response. This response involves both neuronal degeneration and the proliferation of reactive cells. A salient feature of the reactive cells is the co-expression of microglial and astrocytic markers. Surprisingly, the reactive cells also began to express neuronal markers Tubulin βIII and MAP2 adding to the confusion about their origin. Concomitant with their appearance in reactive cells MAP2 and Tubulin βIII expression disappeared from neurons. While NeuN expression decreased significantly, it did not entirely disappear from many neurons. Moreover, it was not observed in reactive cells, showing that NeuN is a reliable marker of neurons",

keywords = "brain injury, epilepsy, human neocortex, neuronal degeneration, neuronal markers, reactive glial cells, tissue culture",

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AU - Verwer, Ronald W. H.

AU - Sluiter, Arja A.

AU - Balesar, Rawien A.

AU - Baayen, Johannes C.

AU - Speijer, Dave

AU - Idema, Sander

AU - Swaab, Dick F.

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N2 - Organotypic cultures from normal neocortical tissue obtained at epilepsy surgery show a severe injury response. This response involves both neuronal degeneration and the proliferation of reactive cells. A salient feature of the reactive cells is the co-expression of microglial and astrocytic markers. Surprisingly, the reactive cells also began to express neuronal markers Tubulin βIII and MAP2 adding to the confusion about their origin. Concomitant with their appearance in reactive cells MAP2 and Tubulin βIII expression disappeared from neurons. While NeuN expression decreased significantly, it did not entirely disappear from many neurons. Moreover, it was not observed in reactive cells, showing that NeuN is a reliable marker of neurons

AB - Organotypic cultures from normal neocortical tissue obtained at epilepsy surgery show a severe injury response. This response involves both neuronal degeneration and the proliferation of reactive cells. A salient feature of the reactive cells is the co-expression of microglial and astrocytic markers. Surprisingly, the reactive cells also began to express neuronal markers Tubulin βIII and MAP2 adding to the confusion about their origin. Concomitant with their appearance in reactive cells MAP2 and Tubulin βIII expression disappeared from neurons. While NeuN expression decreased significantly, it did not entirely disappear from many neurons. Moreover, it was not observed in reactive cells, showing that NeuN is a reliable marker of neurons

KW - brain injury

KW - epilepsy

KW - human neocortex

KW - neuronal degeneration

KW - neuronal markers

KW - reactive glial cells

KW - tissue culture

UR - https://www.scopus.com/pages/publications/84978488787

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DO - 10.1111/bpa.12330

M3 - Article

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Altered Loyalties of Neuronal Markers in Cultured Slices of Resected Human Brain Tissue

Abstract

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Keywords

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