A3 domain region 1803-1818 contributes to the stability of activated factor VIII and includes a binding site for activated factor IX

Esther Bloem; Henriet Meems; Maartje Van Den Biggelaar; Koen Mertens; Alexander B. Meijer

doi:10.1074/jbc.M113.500884

A3 domain region 1803-1818 contributes to the stability of activated factor VIII and includes a binding site for activated factor IX

Esther Bloem
, Henriet Meems
, Maartje Van Den Biggelaar
, Koen Mertens
, Alexander B. Meijer^*

^*Corresponding author for this work

Sanquin Blood Supply Foundation
Utrecht University

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Background: Factor VIII (FVIII) region 1803-1818 may bind activated factor IX (FIXa) or contribute to the stability of activated factor VIII (FVIIIa). Results: Replacing 1803-1810 or 1811-1818 with the homologous regions of factor V decreases FVIIIa stability. FVIII variant F1816A exhibits reduced FIXa binding and FVIIIa stability. Conclusion: FVIII region 1803-1818 is critical for cofactor function. Significance: Novel insight is obtained about FVIII cofactor function.

Original language	English
Pages (from-to)	26105-26111
Number of pages	7
Journal	Journal of biological chemistry
Volume	288
Issue number	36
DOIs	https://doi.org/10.1074/jbc.M113.500884
Publication status	Published - 6 Sept 2013
Externally published	Yes

Access to Document

10.1074/jbc.M113.500884

Cite this

@article{d8d8937df97247a5845faf9ca83648c6,

title = "A3 domain region 1803-1818 contributes to the stability of activated factor VIII and includes a binding site for activated factor IX",

abstract = "Background: Factor VIII (FVIII) region 1803-1818 may bind activated factor IX (FIXa) or contribute to the stability of activated factor VIII (FVIIIa). Results: Replacing 1803-1810 or 1811-1818 with the homologous regions of factor V decreases FVIIIa stability. FVIII variant F1816A exhibits reduced FIXa binding and FVIIIa stability. Conclusion: FVIII region 1803-1818 is critical for cofactor function. Significance: Novel insight is obtained about FVIII cofactor function.",

author = "Esther Bloem and Henriet Meems and \{Van Den Biggelaar\}, Maartje and Koen Mertens and Meijer, \{Alexander B.\}",

year = "2013",

month = sep,

day = "6",

doi = "10.1074/jbc.M113.500884",

language = "English",

volume = "288",

pages = "26105--26111",

journal = "Journal of biological chemistry",

issn = "0021-9258",

publisher = "American Society for Biochemistry and Molecular Biology Inc.",

number = "36",

}

TY - JOUR

T1 - A3 domain region 1803-1818 contributes to the stability of activated factor VIII and includes a binding site for activated factor IX

AU - Bloem, Esther

AU - Meems, Henriet

AU - Van Den Biggelaar, Maartje

AU - Mertens, Koen

AU - Meijer, Alexander B.

PY - 2013/9/6

Y1 - 2013/9/6

N2 - Background: Factor VIII (FVIII) region 1803-1818 may bind activated factor IX (FIXa) or contribute to the stability of activated factor VIII (FVIIIa). Results: Replacing 1803-1810 or 1811-1818 with the homologous regions of factor V decreases FVIIIa stability. FVIII variant F1816A exhibits reduced FIXa binding and FVIIIa stability. Conclusion: FVIII region 1803-1818 is critical for cofactor function. Significance: Novel insight is obtained about FVIII cofactor function.

AB - Background: Factor VIII (FVIII) region 1803-1818 may bind activated factor IX (FIXa) or contribute to the stability of activated factor VIII (FVIIIa). Results: Replacing 1803-1810 or 1811-1818 with the homologous regions of factor V decreases FVIIIa stability. FVIII variant F1816A exhibits reduced FIXa binding and FVIIIa stability. Conclusion: FVIII region 1803-1818 is critical for cofactor function. Significance: Novel insight is obtained about FVIII cofactor function.

UR - https://www.scopus.com/pages/publications/84883723953

U2 - 10.1074/jbc.M113.500884

DO - 10.1074/jbc.M113.500884

M3 - Article

C2 - 23884417

SN - 0021-9258

VL - 288

SP - 26105

EP - 26111

JO - Journal of biological chemistry

JF - Journal of biological chemistry

IS - 36

ER -

A3 domain region 1803-1818 contributes to the stability of activated factor VIII and includes a binding site for activated factor IX

Abstract

Access to Document

Other files and links

Fingerprint

Cite this