A Robust and Clinically Applicable Sample Preparation Protocol for Urinary Extracellular Vesicle Isolation Suitable for Mass Spectrometry-Based Proteomics

Leyla A. Erozenci; Irene V. Bijnsdorp; Sander R. Piersma; Connie R. Jimenez

doi:10.1007/978-1-0716-3457-8_13

A Robust and Clinically Applicable Sample Preparation Protocol for Urinary Extracellular Vesicle Isolation Suitable for Mass Spectrometry-Based Proteomics

Amsterdam UMC
location VUmc

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Urinary extracellular vesicles (uEVs) are a rich source of noninvasive protein biomarkers. However, for translation to clinical applications, an easy-to-use uEV isolation protocol is needed that is compatible with proteomics. Here, we provide a detailed description of a quick and clinical applicable uEV isolation protocol. We focus on the isolation procedure and subsequent in-depth proteome characterization using LC-MS/MS-based proteomics. As an example, we show how differential analyses can be performed using urine samples obtained from prostate cancer patients, compared to urine from controls.

Original language	English
Pages (from-to)	235-251
Number of pages	17
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	2718
DOIs	https://doi.org/10.1007/978-1-0716-3457-8_13
Publication status	Published - 2023

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Clinically applicable
Extracellular vesicles
Isolation of urinary extracellular vesicles
Mass spectrometry
Proteomics
Urine

Access to Document

10.1007/978-1-0716-3457-8_13

Cite this

@article{7d781d7652fd42398fa944f9e8040dad,

title = "A Robust and Clinically Applicable Sample Preparation Protocol for Urinary Extracellular Vesicle Isolation Suitable for Mass Spectrometry-Based Proteomics",

abstract = "Urinary extracellular vesicles (uEVs) are a rich source of noninvasive protein biomarkers. However, for translation to clinical applications, an easy-to-use uEV isolation protocol is needed that is compatible with proteomics. Here, we provide a detailed description of a quick and clinical applicable uEV isolation protocol. We focus on the isolation procedure and subsequent in-depth proteome characterization using LC-MS/MS-based proteomics. As an example, we show how differential analyses can be performed using urine samples obtained from prostate cancer patients, compared to urine from controls.",

keywords = "Clinically applicable, Extracellular vesicles, Isolation of urinary extracellular vesicles, Mass spectrometry, Proteomics, Urine",

author = "Erozenci, \{Leyla A.\} and Bijnsdorp, \{Irene V.\} and Piersma, \{Sander R.\} and Jimenez, \{Connie R.\}",

note = "Publisher Copyright: {\textcopyright} The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023.",

year = "2023",

doi = "10.1007/978-1-0716-3457-8\_13",

language = "English",

volume = "2718",

pages = "235--251",

journal = "Methods in molecular biology (Clifton, N.J.)",

issn = "1064-3745",

publisher = "Humana Press",

}

A Robust and Clinically Applicable Sample Preparation Protocol for Urinary Extracellular Vesicle Isolation Suitable for Mass Spectrometry-Based Proteomics. / Erozenci, Leyla A.; Bijnsdorp, Irene V.; Piersma, Sander R. et al.
In: Methods in molecular biology (Clifton, N.J.), Vol. 2718, 2023, p. 235-251.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - A Robust and Clinically Applicable Sample Preparation Protocol for Urinary Extracellular Vesicle Isolation Suitable for Mass Spectrometry-Based Proteomics

AU - Erozenci, Leyla A.

AU - Bijnsdorp, Irene V.

AU - Piersma, Sander R.

AU - Jimenez, Connie R.

N1 - Publisher Copyright: © The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023.

PY - 2023

Y1 - 2023

N2 - Urinary extracellular vesicles (uEVs) are a rich source of noninvasive protein biomarkers. However, for translation to clinical applications, an easy-to-use uEV isolation protocol is needed that is compatible with proteomics. Here, we provide a detailed description of a quick and clinical applicable uEV isolation protocol. We focus on the isolation procedure and subsequent in-depth proteome characterization using LC-MS/MS-based proteomics. As an example, we show how differential analyses can be performed using urine samples obtained from prostate cancer patients, compared to urine from controls.

AB - Urinary extracellular vesicles (uEVs) are a rich source of noninvasive protein biomarkers. However, for translation to clinical applications, an easy-to-use uEV isolation protocol is needed that is compatible with proteomics. Here, we provide a detailed description of a quick and clinical applicable uEV isolation protocol. We focus on the isolation procedure and subsequent in-depth proteome characterization using LC-MS/MS-based proteomics. As an example, we show how differential analyses can be performed using urine samples obtained from prostate cancer patients, compared to urine from controls.

KW - Clinically applicable

KW - Extracellular vesicles

KW - Isolation of urinary extracellular vesicles

KW - Mass spectrometry

KW - Proteomics

KW - Urine

UR - https://www.scopus.com/pages/publications/85169651818

U2 - 10.1007/978-1-0716-3457-8_13

DO - 10.1007/978-1-0716-3457-8_13

M3 - Article

C2 - 37665463

SN - 1064-3745

VL - 2718

SP - 235

EP - 251

JO - Methods in molecular biology (Clifton, N.J.)

JF - Methods in molecular biology (Clifton, N.J.)

ER -

A Robust and Clinically Applicable Sample Preparation Protocol for Urinary Extracellular Vesicle Isolation Suitable for Mass Spectrometry-Based Proteomics

Abstract

UN SDGs

Keywords

Access to Document

Other files and links

Fingerprint

Cite this