A plasma nucleosome releasing factor (NRF) with serine protease activity is instrumental in removal of nucleosomes from secondary necrotic cells

Sacha Zeerleder; Bas Zwart; Henk te Veldhuis; Rishi Manoe; Ingrid Bulder; Irma Rensink; Lucien A. Aarden

doi:10.1016/j.febslet.2007.10.037

A plasma nucleosome releasing factor (NRF) with serine protease activity is instrumental in removal of nucleosomes from secondary necrotic cells

Sacha Zeerleder
, Bas Zwart
, Henk te Veldhuis
, Rishi Manoe
, Ingrid Bulder
, Irma Rensink
, Lucien A. Aarden

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

We observed that interaction of secondary necrotic (sn) cells with human serum or plasma leads to loss of DNA staining. The decrease turned out to be a result of nucleosome release and was specific for apoptotic cells as necrotic cells did not show this phenomenon. We named this activity in plasma nucleosome releasing factor (NRF). NRF activity was completely inhibited by trypsin inhibitors suggesting that a serine protease is involved. Upon testing a number of plasma candidate serine proteases we found that plasmin did have NRF activity. However, plasminogen-deficient plasma still had NRF activity indicating that NRF is not plasmin. We conclude that a yet unidentified plasma serine protease is involved in removal of nucleosomes from sn cells. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved

Original language	English
Pages (from-to)	5382-5388
Journal	FEBS letters
Volume	581
Issue number	28
DOIs	https://doi.org/10.1016/j.febslet.2007.10.037
Publication status	Published - 2007

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10.1016/j.febslet.2007.10.037

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@article{4346865131d2440d939ecef2aad5e29e,

title = "A plasma nucleosome releasing factor (NRF) with serine protease activity is instrumental in removal of nucleosomes from secondary necrotic cells",

abstract = "We observed that interaction of secondary necrotic (sn) cells with human serum or plasma leads to loss of DNA staining. The decrease turned out to be a result of nucleosome release and was specific for apoptotic cells as necrotic cells did not show this phenomenon. We named this activity in plasma nucleosome releasing factor (NRF). NRF activity was completely inhibited by trypsin inhibitors suggesting that a serine protease is involved. Upon testing a number of plasma candidate serine proteases we found that plasmin did have NRF activity. However, plasminogen-deficient plasma still had NRF activity indicating that NRF is not plasmin. We conclude that a yet unidentified plasma serine protease is involved in removal of nucleosomes from sn cells. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved",

author = "Sacha Zeerleder and Bas Zwart and \{te Veldhuis\}, Henk and Rishi Manoe and Ingrid Bulder and Irma Rensink and Aarden, \{Lucien A.\}",

year = "2007",

doi = "10.1016/j.febslet.2007.10.037",

language = "English",

volume = "581",

pages = "5382--5388",

journal = "FEBS letters",

issn = "0014-5793",

publisher = "Wiley Blackwell",

number = "28",

}

TY - JOUR

T1 - A plasma nucleosome releasing factor (NRF) with serine protease activity is instrumental in removal of nucleosomes from secondary necrotic cells

AU - Zeerleder, Sacha

AU - Zwart, Bas

AU - te Veldhuis, Henk

AU - Manoe, Rishi

AU - Bulder, Ingrid

AU - Rensink, Irma

AU - Aarden, Lucien A.

PY - 2007

Y1 - 2007

N2 - We observed that interaction of secondary necrotic (sn) cells with human serum or plasma leads to loss of DNA staining. The decrease turned out to be a result of nucleosome release and was specific for apoptotic cells as necrotic cells did not show this phenomenon. We named this activity in plasma nucleosome releasing factor (NRF). NRF activity was completely inhibited by trypsin inhibitors suggesting that a serine protease is involved. Upon testing a number of plasma candidate serine proteases we found that plasmin did have NRF activity. However, plasminogen-deficient plasma still had NRF activity indicating that NRF is not plasmin. We conclude that a yet unidentified plasma serine protease is involved in removal of nucleosomes from sn cells. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved

AB - We observed that interaction of secondary necrotic (sn) cells with human serum or plasma leads to loss of DNA staining. The decrease turned out to be a result of nucleosome release and was specific for apoptotic cells as necrotic cells did not show this phenomenon. We named this activity in plasma nucleosome releasing factor (NRF). NRF activity was completely inhibited by trypsin inhibitors suggesting that a serine protease is involved. Upon testing a number of plasma candidate serine proteases we found that plasmin did have NRF activity. However, plasminogen-deficient plasma still had NRF activity indicating that NRF is not plasmin. We conclude that a yet unidentified plasma serine protease is involved in removal of nucleosomes from sn cells. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved

U2 - 10.1016/j.febslet.2007.10.037

DO - 10.1016/j.febslet.2007.10.037

M3 - Article

C2 - 17976375

SN - 0014-5793

VL - 581

SP - 5382

EP - 5388

JO - FEBS letters

JF - FEBS letters

IS - 28

ER -

A plasma nucleosome releasing factor (NRF) with serine protease activity is instrumental in removal of nucleosomes from secondary necrotic cells

Abstract

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